Enzymatic Hydrolysis of Alkaline Pretreated Coconut Coir

Akbarningrum Fatmawati; Rudy Agustriyanto; Yusnita Liasari

doi:10.9767/bcrec.8.1.4048.34-39

Enzymatic Hydrolysis of Alkaline Pretreated Coconut Coir

*Akbarningrum Fatmawati - Department of Chemical Engineering, Surabaya University, Jl. Raya Kalirungkut, Surabaya 60292,, Indonesia

Rudy Agustriyanto - Department of Chemical Engineering, Surabaya University, Jl. Raya Kalirungkut,, Indonesia

Yusnita Liasari - Biotechnology Faculty, Surabaya University, Jl. Raya Kalirungkut, Surabaya 60292,, Indonesia

Received: 2 Oct 2012; Published: 19 Jun 2013.

DOI: https://doi.org/10.9767/bcrec.8.1.4048.34-39 View

BibTex Citation Data :

@article{BCREC4048,
    author = {Akbarningrum Fatmawati and Rudy Agustriyanto and Yusnita Liasari},
    title = {Enzymatic Hydrolysis of Alkaline Pretreated Coconut Coir},
    journal = {Bulletin of Chemical Reaction Engineering & Catalysis},
  volume = {8},
    number = {1},
    year = {2013},
    keywords = {coconut; enzyme; hydrolysis; lignocellulose},
    abstract = {The  purpose of this research is to study the effect of concentration and temperature on the cellulose and lignin content, and the reducing sugars produced in the enzymatic hydrolysis of coconut coir. In this research, the coconut coir is pretreated using 3%, 7%, and 11% NaOH solution at 60oC, 80oC, and 100oC. The pretreated coir were assayed by measuring the amount of cellulose and lignin and then hydrolysed using Celluclast and Novozyme 188 under various temperature (30oC, 40oC, 50oC) and pH (3, 4, 5). The hydrolysis results were assayed for the reducing sugar content. The results showed that the alkaline delignification was effective to reduce lignin and to increase the cellulose content of the coir. The best delignification condition was observed at 11% NaOH solution and 100oC which removed 14,53% of lignin and increased the cellulose content up to 50,23%. The best condition of the enzymatic hydrolysis was obtained at 50oC and pH 4 which produced 7,57 gr/L reducing sugar. © 2013 BCREC UNDIP. All rights reserved  Received: 2nd October 2012; Revised: 31st January 2013; Accepted: 6th February 2013   [ How to Cite : Fatmawati, A., Agustriyanto, R., Liasari, Y. (2013). Enzymatic Hydrolysis of Alkaline Pre-treated Coconut Coir.   Bulletin of Chemical Reaction Engineering & Catalysis  , 8 (1): 34-39 (doi:10.9767/bcrec.8.1.4048.34-39)  [ Permalink/DOI :  http://dx.doi.org/10.9767/bcrec.8.1.4048.34-39 ]      |  View in    |     },
   issn = {1978-2993},   pages = {34--39}  doi = {10.9767/bcrec.8.1.4048.34-39},
    url = {https://ejournal.undip.ac.id/index.php/bcrec/article/view/4048}
}

Citation Format:

Abstract

The purpose of this research is to study the effect of concentration and temperature on the cellulose and lignin content, and the reducing sugars produced in the enzymatic hydrolysis of coconut coir. In this research, the coconut coir is pretreated using 3%, 7%, and 11% NaOH solution at 60oC, 80oC, and 100oC. The pretreated coir were assayed by measuring the amount of cellulose and lignin and then hydrolysed using Celluclast and Novozyme 188 under various temperature (30oC, 40oC, 50oC) and pH (3, 4, 5). The hydrolysis results were assayed for the reducing sugar content. The results showed that the alkaline delignification was effective to reduce lignin and to increase the cellulose content of the coir. The best delignification condition was observed at 11% NaOH solution and 100oC which removed 14,53% of lignin and increased the cellulose content up to 50,23%. The best condition of the enzymatic hydrolysis was obtained at 50oC and pH 4 which produced 7,57 gr/L reducing sugar. © 2013 BCREC UNDIP. All rights reserved

Received: 2nd October 2012; Revised: 31st January 2013; Accepted: 6th February 2013

[How to Cite: Fatmawati, A., Agustriyanto, R., Liasari, Y. (2013). Enzymatic Hydrolysis of Alkaline Pre-treated Coconut Coir. Bulletin of Chemical Reaction Engineering & Catalysis, 8 (1): 34-39 (doi:10.9767/bcrec.8.1.4048.34-39)

[Permalink/DOI: http://dx.doi.org/10.9767/bcrec.8.1.4048.34-39]

| View in |

Keywords: coconut; enzyme; hydrolysis; lignocellulose

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Section: Original Research Articles

Language: EN

In 2013: BCREC Volume 8 Issue 1 Year 2013 (SCOPUS Indexed, June 2013)

Statistics: 2144 2068

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