Ethanol Production from Non-Food Tubers of Iles-iles (Amorphophallus campanulatus) by Using Separated Hydrolysis and Fermentation

*Kusmiyati Kusmiyati  -  (SCOPUS h-index: 2); Renewable Energy Research Centre, Department of Chemical Engineering, Faculty of Engineering, Muhammadiyah Surakarta University, Jl. A. Yani Tromol Pos 1 Pabelan Kartasura 57102 Telp 0271 717417, Surakarta,, Indonesia
Received: 7 Jan 2014; Published: 1 Aug 2014.
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Abstract

The decrease in production and the raise in needs have led to the rise in oil prices. This work investigated the possibility of Iles-iles (Amorphophallus campanulatus) tuber flour, which is rich in carbohydrate con-tent, as a raw material to produce bioethanol. To obtain the maximum ethanol concentration, several parameters had been studied, such as: the concentration of α-amylase and β-amylase in liquefaction and sac-charification processes, respectively, the type of S. cerevisiae enzyme (pure, dry, wet and instant) and weight of Diammonium phosphate (DAP) as a nutrient for S. cerevisiae in fermentation. The result shows that the highest reducing sugar content (12.5%) was achieved when 3.2 ml α-amylase/kg flour and 6.4 ml β-amylase/kg flour were used during liquefaction and saccharification processes. Since the concentration of α- and β-amylase increased, the reducing sugar obtained also increased. The higher sugar content resulted the higher the ethanol concentration in the fermentation broth. Furthermore, the highest concentration of ethanol (9 %v/v) was obtained at 72 h fermentation using the dry S. cerevisiae, at 3.2 ml and 6.4 ml /kg flour of α-amylase and β-amylase enzymes, respectively. From the study of the effect of S. cerevisiae type, it was shown that dry S. cereviseae produced the highest ethanol concentration 10.2% (v/v) at 72 h fermentation. The DAP was used as a nitrogen supply required by S. cerevisiae to growth and as a results can increase the ethanol concentration. The addition of DAP in the fermentation proved that 8.45% (v/v) of ethanol was obtained. This result shows that the proposed tuber flour has the potential a raw material for bioethanol production. © 2014 BCREC UNDIP. All rights reserved

Received: 7th January 2014; Revised: 10th March 2014; Accepted: 18th March 2014

[How to Cite: Kusmiyati, K. (2014). Ethanol Production from Non-Food Tubers of Iles-iles (Amorphophallus campanulatus) by using Separated Hydrolysis and Fermentation. Bulletin of Chemical Reaction Engineering & Catalysis, 9 (2): 93-99. (doi:10.9767/bcrec.9.2.6014.93-99)]

[Permalink/DOI: http://dx.doi.org/10.9767/bcrec.9.2.6014.93-99]


Keywords: Biofuel; Bioethano; iles-iles; S. cerevisiae; α amylase; β amylase
Funding: DIKTI

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Article Info
Section: Original Research Articles
Language: EN
In 2014: BCREC Volume 9 Issue 2 Year 2014 (SCOPUS Indexed, August 2014)
Statistics: 1956 1753
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