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Ethanol Production from Non-Food Tubers of Iles-iles (Amorphophallus campanulatus) by Using Separated Hydrolysis and Fermentation

*Kusmiyati Kusmiyati  -  (SCOPUS h-index: 2); Renewable Energy Research Centre, Department of Chemical Engineering, Faculty of Engineering, Muhammadiyah Surakarta University, Jl. A. Yani Tromol Pos 1 Pabelan Kartasura 57102 Telp 0271 717417, Surakarta,, Indonesia

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Abstract

The decrease in production and the raise in needs have led to the rise in oil prices. This work investigated the possibility of Iles-iles (Amorphophallus campanulatus) tuber flour, which is rich in carbohydrate con-tent, as a raw material to produce bioethanol. To obtain the maximum ethanol concentration, several parameters had been studied, such as: the concentration of α-amylase and β-amylase in liquefaction and sac-charification processes, respectively, the type of S. cerevisiae enzyme (pure, dry, wet and instant) and weight of Diammonium phosphate (DAP) as a nutrient for S. cerevisiae in fermentation. The result shows that the highest reducing sugar content (12.5%) was achieved when 3.2 ml α-amylase/kg flour and 6.4 ml β-amylase/kg flour were used during liquefaction and saccharification processes. Since the concentration of α- and β-amylase increased, the reducing sugar obtained also increased. The higher sugar content resulted the higher the ethanol concentration in the fermentation broth. Furthermore, the highest concentration of ethanol (9 %v/v) was obtained at 72 h fermentation using the dry S. cerevisiae, at 3.2 ml and 6.4 ml /kg flour of α-amylase and β-amylase enzymes, respectively. From the study of the effect of S. cerevisiae type, it was shown that dry S. cereviseae produced the highest ethanol concentration 10.2% (v/v) at 72 h fermentation. The DAP was used as a nitrogen supply required by S. cerevisiae to growth and as a results can increase the ethanol concentration. The addition of DAP in the fermentation proved that 8.45% (v/v) of ethanol was obtained. This result shows that the proposed tuber flour has the potential a raw material for bioethanol production. © 2014 BCREC UNDIP. All rights reserved

Received: 7th January 2014; Revised: 10th March 2014; Accepted: 18th March 2014

[How to Cite: Kusmiyati, K. (2014). Ethanol Production from Non-Food Tubers of Iles-iles (Amorphophallus campanulatus) by using Separated Hydrolysis and Fermentation. Bulletin of Chemical Reaction Engineering & Catalysis, 9 (2): 93-99. (doi:10.9767/bcrec.9.2.6014.93-99)]

[Permalink/DOI: http://dx.doi.org/10.9767/bcrec.9.2.6014.93-99]


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Keywords: Biofuel; Bioethano; iles-iles; S. cerevisiae; α amylase; β amylase
Funding: DIKTI

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  1. Koike, Y., Tang, Y.Q., Syo, T., Osaka, N., Morimura, S., and Kida, K. (2009). Production of Fuel Ethanol and Methane from Garbage by High Efficiency Two Stage Fermentation Process. Journal of Bioscience and Bioengineering. 108: 508–512
  2. Meher, L.C., Sagar, D.V., Naik, S.N. (2006). Technical Aspects of Biodiesel Production Transesterification - A Review. Renewable and Sustainable Energy. 10: 248–68
  3. Li, H., Kim, N.G., Jiang, M., Kan, J.W., Chang, H.N. (2009). Simultaneous Saccharification and Fermentation of Lignocellulosic Residues Pretreated with Phosphoric Acid–Acetone for Bioethanol Production. Bioresource Technology. 100: 3245–3251
  4. Darmawan, E. (2010). Singkong Sambung sebagai Bahan Bakar Alternatif Masa Depan, http://ladangsawah.blogspot.com/2010/04/judul-singkong-sambung-sebagai-bahan.html (in Indonesian)
  5. Schell, D.J., Riley, C.J., Dowe, N., Farmer, J., Ibsen, K.N., Ruth, M.F., Toon, S.T., Lumpkin, R.E. (2004). A Bioethanol Process Development Unit: Initial Operating Experiences and Results with a Corn Fiber Feedstock. Bioresource Technology. 91: 179–188
  6. Yu, Z., Zhang, H. (2003). Ethanol Fermentation of Acid-Hydrolyzed Cellulosic Pyrolysate with Saccharomyces cerevisiae. Bioresource Technology. 90: 95–100
  7. Saha, B.C., Iten, L.B., Cotta, M.A., Wu, Y.V. (2005). Dilute Acid Pretreatment, Enzymatic Saccharification and Fermentation of Wheat Straw to Ethanol. Process Biochemistry. 40: 3693–3700
  8. Koike, Y., Tang, Y.Q., Syo, T., Osaka, N., Morimura, S., Kida, K. (2009). Production of Fuel Ethanol and Methane from Garbage by High Efficiency Two Stage Fermentation Process. Journal of Bioscience and Bioengineering. 108: 508–512
  9. Shavanas, S., Padmaja, G., Moorthy, S.N., Sajeev, M.S., Sheriff, M.S. (2011). Process Opti-mization for Bioethanol Production from Cassava Starch using Novel Eco-Friendly Enzymes. Biomass and Bioenergy. 35: 901-909
  10. Oscar, J.S., Carlos, A.C. (2008). Trends in Biotechnological Production of Fuel Ethanol from Different Feedstocks. Bioresource Technology. 99: 5270-5295
  11. Siqueira, P.F., Karp, G.P., Carvalho, J.C., Sturm, W., José, A., León, R., Tholozan, J.L., Singhania, R., Pandey, A., Soccol, C.R. (2008). Production of Bio-Ethanol from Soybean Molasses By Saccharomyces cerevisiae at Laboratory, Pilot and Industrial Scales. Bioresource Technology. 99: 8156–8163
  12. Whitaker, J.R. (1996). Enymes In: Food Chemistry. O.R. Fennema. M. Dekker, New York
  13. Wu, X., Staggenborg, S., Propheter, J.L., Rooney, W.L., Yu, J., Wang, D. (2009). Features of Sweet Sorghum Juice and Their Performance in Ethanol Fermentation. Industrial Crops and Products. 31: 164–170
  14. Silverstein, R.A., Ye, C., Ratna, R.M., Sharma, S., Boyette, M.D., Jason, O. (2007). A comparison of Chemical Pretreatment Methods for Improving Saccharification of Cotton Stalks. Bioresource Technology. 98: 3000-3011
  15. Balat, M. (2011). Production of Bioethanol from Lignocellulosic Materials via The Bio-chemical Pathway: A review. Energy Conversion and Management. 52: 858–875
  16. Sharma, S.K., Kalra, K.L., Grewal, H.S. (2002). Fermentation of Enzymatically Saccharified Sunflower Stalks for Ethanol Production and Its Scale Up. Bioresource Technology. 85: 31–33
  17. Shavanas, S., Padmaja, G., Moorthy, S.N., Sajeev, M.S., Sheriff, M.S. (2011). Process Optimization for Bioethanol Production from Cassava Starch using Novel Eco-Friendly Enzymes. Biomass and Bioenergy 35: 901-909
  18. Bafrncova, P., Smogrovicova, D., Slavikova, I., Patpova, J., Domeny, Z. (1999). Improvement of Very High Gravity Ethanol Fermentation by Media Supplementation Using Saccharomyces Cerevisiae. Biotechnology Letters 21: 337-441

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