skip to main content

Immobilization of Pepsin onto Chitosan Silica Nanobeads with Glutaraldehyde as Crosslink Agent

*Sari Edi Cahyaningrum  -  Department of chemistry, Faculty of mathematics and natural science State, Surabaya University, Indonesia
Maria Monica Sianita  -  Department of chemistry, Faculty of mathematics and natural science State, Surabaya University, Indonesia

Citation Format:
Cover Image
Abstract

Glutaraldehyde crosslinked chitosan - silica nanobeads have been used as a support in the immobiliza-tion process of pepsin. The effect of pH, pepsin concentration, and temperature on the characteristics of both free and immobilized pepsin had been investigated in this study. The results showed that the im-mobilized pepsin has wider pH range, better capacity, performance and stability to high temperatures compared with those of free pepsin. The immobilized pepsin can be used for 8 to 10 times at which the activity remains 20%, whereas the free pepsin can only be used once. These results suggested promis-ing applications of immobilized enzyme as biocatalyst. © 2014 BCREC UNDIP. All rights reserved.

Received: 1st July 2014; Revised: 3rd September 2014; Accepted: 3rd September 2014

How to Cite: Cahyaningrum, S.E., Sianita, M.M. (2014). Immobilization of Pepsin onto Chitosan Silica Nanobeads with Glutaraldehyde as Crosslink Agent. Bulletin of Chemical Reaction Engineering & Catalysis, 9 (3): 263-269. (doi:10.9767/bcrec.9.3.7060.263-269)

Permalink/DOI: http://dx.doi.org/10.9767/bcrec.9.3.7060.263-269

Fulltext View|Download
Keywords: immobilized pepsin; chitosan; silica; glutaraldehyde crosslinker
Funding: department of chemistry

Article Metrics:

Article Info
Section: Original Research Articles
Language : EN
Statistics:
Share:
  1. Ahmed S.A.A. (2003). Biochemical Studies on Some Enzymes Used in Industry. Ph.D Thesis. Cario University, Faculty of Agriculture, Cairo: 109-135
  2. Magda A. El- Bendary, Maysa E.Moharam, and Thanaa H.Ali. (2009). Efficient Immobilization of Milk Clotting Enzyme produced by Bacillus sphaericus. Polish Journal of Food and Nutrition Sciences, 59:167-72
  3. El Bndary M.A., Moharam M.A., and Ali T.H. (2007). Purification and Characterization of Milk Clotting Activity by Bacillus sphaericus. Egypt. Journal Applied Sciences Reseach, 3: 695-699
  4. Frydlova, J., Kucerova, Z., & Ticha. (2004). Affinity Chromatography of Porcine Pepsin and Pepsinogen using Immobilized Ligand Derived from the Specific Substrate for this Enzyme. Journal of chromatography B, 800: 109-114
  5. Altum, G.D and Senay A. C., (2007). Immobilization Of Pepsin On Chitosan Beads. Food Chemistry. 100: 964-971
  6. Esawy M.A and Combet Blanc Y., (2006). Immobilization of Bacillus licheniformis 5A1 Milk Clotting Enzymes and Characterization of its Enzymme Properties. World. Journal Microbiology Biotechnology. 22: 197-200
  7. Orrego, C.E., Salgado, J.S. Valencia, G.I. Gialdo and C.A. Cardona (2010). Novel Chitosan Membranes as Support for Lipases Immobilization: Characterization Aspects. Carbohydrates Polymer. 79:9-16
  8. Malmiri, Hoda Jafarizadeh, Jahanian, Mohammad Ali Ghaz and Berenjian, Aydin, 2012. Potential Applications Of Chitosan NanoparticlesAs Novel Support In Enzymee Immobilization. American Journal of Biochemistry and Biotechnology. 8 (4): 203-219
  9. Chiou, S. H., and Wu, W.T., (2004). Immobilization of Candida rugosa Lipase on Chitosan with Activation of the Hydroxyl Groups. Biomaterials, 25:197-204
  10. Chao, L., langen, L.V., and Sheldon, R.A., (2003). Immobilization Enzymes: Carrier-bound or Carrier Free? Current Opinion in Biotechnology, 14:387-394
  11. Wenshan Liu, Liang Wang and Rongrong Jiang. (2012). Specific Enzyme Immobilization Approaches and Their Application with Nanomaterials. Top Catalysis 55: 1146-1156
  12. Ticu E.L, Marko, D., froindevaux, Huma, Artenie, V., & Guillochon, D., (2005). Use Of Protease –Modified –Alumina Complex To Design A Continuous Stirred Tank Reactor For Producing Bioactive Hydrolysates. Process Biochemistry, 40 (8): 2841-2848
  13. Godjevargova T., and Nenkova. (2006). Immobilization of Glucode Oxidase by Acrylonitrile Copolymer Coated Silica Supports. Journal Molecules Catalysis B- Enzyme. 38: 59-64
  14. Basarani Devi, B.G. Unni, S.B.Wann and R. Samanta. (2012). Immobilization of Partially Purified Alpha Amylase Enzyme Produced by Soil Born Bacillus sp. Advances in Apllied Science Reseach. 3(5): 2739-2744
  15. Valentina, N., S. Michela and G. Elena, (2011). Β-Galactosidase Entrapment in Silica Gel Matrices for a More Effective Treatment of Lactose Intolerance. Journal Molecular . Catalysis Biological Enzyme. 143: 62-72
  16. Chen He, Qiuhong Zang, Yichao Dang & Guiwei Shu. (2013). The Effect of Glutaraldehyde Cross-Linking in the Enzyme Activity of Immobilized β- Galactosidase on Chitosan Beads. Advance Journal of Food Science and Techology. 5.(7 ) : 932-925
  17. Jiammin Wu, Mingming Luang and Ziayin Lhao. (2006). Trypsin Imobilization by Direct Adsorption on Metel Ion Chelated Macroporous Chitosan Silica Gel Beads.International Journal of Biological Macromolecules.39: 185-191
  18. Laing, Z., Sang, J.Li, C. Cao and G. Wang. (2006). Preparation of Urease Immobilized onto Glutaraldehyde Cross-linked Chitosan Bead. Journal Qindai Univ. Science. Technology., 27: 123-126
  19. Cahyaningrum, S.E., Narsito, Santoso, S.J., and Agustini, R (2009). Involvement Of Metal Cation Crosslink Agent In Papain Immobilization On Chitosan. PhD Dissertation, Dept. of Chemistry. Gajah Mada University. Indonesia
  20. Bradford, (1976) A Rapid And Sensitive Method for Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein Dye Binding. Analytical Biochemistry. 72: 248

Last update:

No citation recorded.

Last update: 2021-09-20 17:37:54

  1. Fabrication of nanohydroxyapatite/ scrawl collagen/chitosan composite for bone graft candidate

    Cahyaningrum S.E.. Rasayan Journal of Chemistry, 11 (2), 2018. doi: 10.7324/RJC.2018.1121916
  2. Capacity and kinetic adsorption calcium metal ion on chitosan nano beads

    Cahyaningrum S.. Research Journal of Pharmaceutical, Biological and Chemical Sciences, 7 (3), 2016.
  3. Green Synthesis of Porous Spherical Reduced Graphene Oxide and Its Application in Immobilized Pectinase

    Gao M.. ACS Omega, 2021. doi: 10.1021/acsomega.0c05078
  4. Nanocarriers immobilized proteases and their industrial applications: An overview

    Husain Q.. Journal of Nanoscience and Nanotechnology, 18 (1), 2018. doi: 10.1166/jnn.2018.15246
  5. Synthesis and characterization chitosan-glutaraldehide alginate blends for candidate hemodialysis membrane

    Cahyaningrum S.. Rasayan Journal of Chemistry, 10 (3), 2017. doi: 10.7324/RJC.2017.1031635
  6. Imprinting "nano-SiO2-Crosslinked Chitosan-Nano-TiO2" Polymeric Nanocomposite for Selective and Instantaneous Microwave-Assisted Sorption of Hg(II) and Cu(II)

    Mahmoud M.. ACS Sustainable Chemistry and Engineering, 6 (4), 2018. doi: 10.1021/acssuschemeng.7b03215