DOI: https://doi.org/10.9767/bcrec.10.3.8511.230-236

Production of Oleic Acid Ethyl Ester Catalyzed by Crude Rice Bran (Oryza sativa) Lipase in a Modified Fed-batch System: A Problem and its Solution

*Indro Prastowo  -  Department of Biology Education, Faculty of Teacher Training and Education, Ahmad Dahlan University, Kampus III, Jl. Prof. Dr. Soepomo, Janturan, Yogyakarta 55164, Indonesia
Chusnul Hidayat  -  Department of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Gadjah Mada University, Jl. Sosio Justisia, Bulaksumur, Yogyakarta 55281, Indonesia
Pudji Hastuti  -  Department of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Gadjah Mada University, Jl. Sosio Justisia, Bulaksumur, Yogyakarta 55281, Indonesia

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Abstract

A fed-batch system was modified for the enzymatic production of Oleic Acid Ethyl Ester (OAEE) using rice bran (Oryza sativa) lipase by retaining the substrate molar ratio (ethanol/oleic acid) at 2.05:1 during the reaction. It resulted in an increase in the ester conversion of up to 76.8% in the first 6 h of the reaction, which was then followed by a decrease from 76.8% to 22.9% in 6 h later. The production of water in the reaction system also showed a similar trend. The water was hypothesized to lead lipase to reverse the reaction which resulted in a decrease in both (water and esters) in the last 6 h of the reac- tion. In order to overcome the problem, zeolite powder (25 and 50 mg/mL) were added into the reaction system at 5 h of the reaction. As the result, the final ester conversions increased drastically up to 90 - 95.7%. Thus, the combination of a constant substrate molar ratio (ethanol/oleic acid) during the reaction (at 2.05:1) with the addition of zeolite powder (25 and 50 mg/mL) to the reaction system at 5 h is effective for the enzymatic synthesis of OAEE. © 2015 BCREC UNDIP. All rights reserved

Received: 2nd May 2015; Revised: 20th June 2015; Accepted: 2nd July 2015

How to Cite: Prastowo, I., Hidayat, C., Hastuti, P. (2015). Production of Oleic Acid Ethyl Ester Catalyzed by Crude Rice Bran (Oryza sativa) Lipase in a Modified Fed-batch System: A Problem and its Solution. Bulletin of Chemical Reaction Engineering & Catalysis, 10 (3): 230-236. (doi:10.9767/bcrec.10.3.8511.230-236)

Permalink/DOI: http://dx.doi.org/10.9767/bcrec.10.3.8511.230-236

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Keywords: Fed-batch system; rice bran (Oryza sativa) lipase; substrate molar ratio (ethanol/oleic acid); water; zeolite powder

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