Induksi Kalus Binahong (Basella rubra L.) Secara In Vitro Pada Media Murashige & Skoog Dengan Konsentrasi Sukrosa Yang Berbeda

Abstract

Binahong (Basella rubra L.) is a plant medicine consisting secondary metabolites which have virtue as
medicines for several diseases that could also be used as coloring agent. The medicine compounds in secondary
metabolites could be extracted from callus. Sucrose is one of the components that build MS (Murashige & Skoog)
medium. Sucrose is important in in vitro culture, it functions as carbon and energy source for explant to grow. The
purposes of this research are to study the effect of sucrose in MS medium towards B. rubra L callus formation and
growth; to find the optimum sucrose concentration for callus B. rubra L formation; and also to find the fastest
initiation time to produce callus crumb. This research uses Complete Random Design (CRD) single factor method,
i.e., sucrose concentration of 0 g/l, 10g/l, 20 g/l, 30 g/l, 40 g/l with five repetitions. The data is analysed with
Analysis of Varian (Anova) and if a real difference is found the analysis is continued with Duncan Multiple Range
Test (DMRT) with significancy level of 95%. The results show that various sucrose concentrations in MS medium
influences callus B. rubra L induction. The highest sucrose concentration, i.e. 40 g/l, which was added into MS
medium, could induce the maximum callus wet-weight of 1,69 g and the fastest callus initiation time of 4,8 day.