1Food Technology Department, Faculty of Agricultural Technology , Indonesia
2Soegijapranata Catholic University (SCU), UNIKA, Indonesia
3Biotechnology Study Program, Faculty of Science and Mathematics, , Indonesia
4 Diponegoro University, Indonesia
5 School of Pharmacy, Bandung Institute of Technology, Bandung, Indonesia
6 Department of Biology, Institut Teknologi Sepuluh November, Surabaya, Indonesia
7 Molecular Applied Microbiology Laboratory , UPT. Terpadu Laboratory Diponegoro University, Indonesia
BibTex Citation Data :
@article{Bioma51577, author = {Dyah Wulandari and Giwang Dewantoro and Arina Tri Lunggani and Agung Suprihadi and Catur Riani and Edwin Setiawan and Siti Lutfiatul Farikha and Anto Budiharjo}, title = {Antibacterial Activity of Freshwater Sponge Oncosclera asiatica Against Escherichia coli and Staphylococcus aureus}, journal = {Bioma : Berkala Ilmiah Biologi}, volume = {24}, number = {2}, year = {2023}, keywords = {Oncosclera asiatica, antibacterial activity, Pseudomonas moraviensis, NRPS gene, bacterial symbiont}, abstract = { Freshwater sponges are animals from the Porifera phylum that live in freshwater. The sponge used is Oncosclera asiatica was taken from Kali Porong, East Java. Seventeen isolates of bacteria have been obtained from isolation . Antibacterial potential testing was performed by paper disc inhibition assay using Escherichia coli and Staphylococcus aureus as pathogenic bacteria and amoxicillin as a positive control. The antibacterial activity test showed that four isolates have the potential activity. The isolates with the highest inhibition zones were identified using a 16S rRNA.The results of BLAST showed isolate number 2 was Pseudomonas moraviensis with 99.51% similarity. The phylogenetic tree analysis was build using the MEGA X program. The results of the phylogenetic tree analysis showed that P.moraviensis had a bootstrap value of 100% with a genetic distance value of 0.001. P. moraviensis isolates screened for the presence of Non-Ribosomal Peptide Synthetase (NRPS) gene by A2gamF and A3gamR primers. The amplification result from NRPS gene showed positive meaning that P.moraviensis genome contained NRPS gene. }, issn = {2598-2370}, pages = {120--129} doi = {10.14710/bioma.24.2.120-129}, url = {https://ejournal.undip.ac.id/index.php/bioma/article/view/51577} }
Refworks Citation Data :
Freshwater sponges are animals from the Porifera phylum that live in freshwater. The sponge used is Oncosclera asiatica was taken from Kali Porong, East Java. Seventeen isolates of bacteria have been obtained from isolation. Antibacterial potential testing was performed by paper disc inhibition assay using Escherichia coli and Staphylococcus aureus as pathogenic bacteria and amoxicillin as a positive control. The antibacterial activity test showed that four isolates have the potential activity. The isolates with the highest inhibition zones were identified using a 16S rRNA.The results of BLAST showed isolate number 2 was Pseudomonas moraviensis with 99.51% similarity. The phylogenetic tree analysis was build using the MEGA X program. The results of the phylogenetic tree analysis showed that P.moraviensis had a bootstrap value of 100% with a genetic distance value of 0.001. P. moraviensis isolates screened for the presence of Non-Ribosomal Peptide Synthetase (NRPS) gene by A2gamF and A3gamR primers. The amplification result from NRPS gene showed positive meaning that P.moraviensis genome contained NRPS gene.
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