1Jurusan Ilmu Kelautan, Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro, Indonesia
2Program Studi Bioteknologi, Sekolah Pasca Sarjana, Universitas Gadjah Mada
3Jurusan Ilmu Kelautan, Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro
BibTex Citation Data :
@article{IK.IJMS11110, author = {Subagiyo Subagiyo and Sebastian Margino and Triyanto Triyanto and Wilis Ari Setyati1,2}, title = {Effects Of pH, Temperature And Salinity In Growth And Organic Acid Production Of Lactic Acid Bacteria Isolated From Penaeid Shrimp Intestine}, journal = {ILMU KELAUTAN: Indonesian Journal of Marine Sciences}, volume = {20}, number = {4}, year = {2015}, keywords = {}, abstract = { Bakteri asam laktat telah lama dikembangkan sebagai probiotik. Penentuan kondisi lingkungan yang optimum untuk pertumbuhan sel serta asam organik memberikan gambaran aktivitas optimum untuk kinerja probiotik baik dalam sistem fisiologi inang maupun dalam sistem bioproses untuk produksi sel dan metabolit. Penelitian ini bertujuan untuk mengetahui pengaruh faktor lingkungan (pH, suhu dan salinitas) terhadap pertumbuhan dan produksi total asam organik tiga isolat bakteri asam laktat yang telah diseleksi dari intestinum udang penaeid. Eksperimen menggunakan medium deMan, Rogosa and Sharpe (MRS) cair. Perlakuan pH awal meliputi nilai pH 4, 5 dan 6. Perlakuan suhu meliputi suhu 25, 30 dan 35 O C serta perlakuan salinitas meliputi salinitas 0,75 %, 1,5 % dan 3 %. Setiap interval 6 jam dilakukan pengambilan sampel kultur bakteri dan penghitungan pertumbuhan berdasarkan perubahan optical density (pada panjang gelombang 600 nm) sedangkan produksi asam laktat dianalisis dengan metode titrimetrik menggunakan NaOH 1 N sebagai larutan titrasinya. Berdasarkan hasil penelitian disimpulkan bahwa suhu, pH awal dan salinitas berpengaruh terhadap pertumbuhan dan produksi asam organik. Nilai kondisi lingkungan terbaik untuk pertumbuhan dapat berbeda dengan nilai terbaik untuk produksi asam organic. Hal ini ditunjukan oleh nilai laju pertumbuhan dan produksi asam laktat tertinggi dari tiga isolat uji terjadi pada suhu, pH awal dan salinitas yang berbeda. Isolat L12 tumbuh optimum pada suhu 30 o C, pH awal 6 dan salinitas 0,75%. Isolat L14 tumbuh optimum pada suhu 30 o C, pH awal 6 dan salinitas 1.5%. Isolat L 21 tumbuh optimum pada suhu 30 o C, pH awal 6 dan salinitas 1.5%. Kata kunci: bakteri asam laktat, suhu, pH, salinitas, asamorganik, pertumbuhan, Lactic acid bacteria are widely distributed in intestinal tracts of various animals where they live as normal flora.Strains of lactic acid bacteria are the most common microbes employed as probiotics , The optimum condition for growth are important to mass production and to determined parameters most suitable for growth. The effects of temperature, pH and salinity on the growth and production of lactic acid from the three shrimp intestinal lactic acid bacteria isolates were conducted using bacth culture in a flask. These variables for growth were determined based on the growth curves and lactic acid production. Data from the flask batch experiment demonstrated that the best initial pH and temperature for growth of isolat L12 ,L14 and L21 were found to be pH 6 and 30 O C. Salinity (NaCl concentration) 0,75% were the best for growth of isolat L12. Salnity 1,5 % were best for growth of isolat L14 and L21. Key words : growth, temperature, pH, salinity, lactic aid bacteria }, issn = {2406-7598}, pages = {187--194} doi = {10.14710/ik.ijms.20.4.187-194}, url = {https://ejournal.undip.ac.id/index.php/ijms/article/view/11110} }
Refworks Citation Data :
Bakteri asam laktat telah lama dikembangkan sebagai probiotik. Penentuan kondisi lingkungan yang optimum untuk pertumbuhan sel serta asam organik memberikan gambaran aktivitas optimum untuk kinerja probiotik baik dalam sistem fisiologi inang maupun dalam sistem bioproses untuk produksi sel dan metabolit. Penelitian ini bertujuan untuk mengetahui pengaruh faktor lingkungan (pH, suhu dan salinitas) terhadap pertumbuhan dan produksi total asam organik tiga isolat bakteri asam laktat yang telah diseleksi dari intestinum udang penaeid. Eksperimen menggunakan medium deMan, Rogosa and Sharpe (MRS) cair. Perlakuan pH awal meliputi nilai pH 4, 5 dan 6. Perlakuan suhu meliputi suhu 25, 30 dan 35OC serta perlakuan salinitas meliputi salinitas 0,75 %, 1,5 % dan 3 %. Setiap interval 6 jam dilakukan pengambilan sampel kultur bakteri dan penghitungan pertumbuhan berdasarkan perubahan optical density (pada panjang gelombang 600 nm) sedangkan produksi asam laktat dianalisis dengan metode titrimetrik menggunakan NaOH 1 N sebagai larutan titrasinya. Berdasarkan hasil penelitian disimpulkan bahwa suhu, pH awal dan salinitas berpengaruh terhadap pertumbuhan dan produksi asam organik. Nilai kondisi lingkungan terbaik untuk pertumbuhan dapat berbeda dengan nilai terbaik untuk produksi asam organic. Hal ini ditunjukan oleh nilai laju pertumbuhan dan produksi asam laktat tertinggi dari tiga isolat uji terjadi pada suhu, pH awal dan salinitas yang berbeda. Isolat L12 tumbuh optimum pada suhu 30oC, pH awal 6 dan salinitas 0,75%. Isolat L14 tumbuh optimum pada suhu 30oC, pH awal 6 dan salinitas 1.5%. Isolat L 21 tumbuh optimum pada suhu 30 oC, pH awal 6 dan salinitas 1.5%.
Kata kunci: bakteri asam laktat, suhu, pH, salinitas, asamorganik, pertumbuhan,
Lactic acid bacteria are widely distributed in intestinal tracts of various animals where they live as normal flora.Strains of lactic acid bacteria are the most common microbes employed as probiotics, The optimum condition for growth are important to mass production and to determined parameters most suitable for growth. The effects of temperature, pH and salinity on the growth and production of lactic acid from the three shrimp intestinal lactic acid bacteria isolates were conducted using bacth culture in a flask. These variables for growth were determined based on the growth curves and lactic acid production. Data from the flask batch experiment demonstrated that the best initial pH and temperature for growth of isolat L12 ,L14 and L21 were found to be pH 6 and 30 OC. Salinity (NaCl concentration) 0,75% were the best for growth of isolat L12. Salnity 1,5 % were best for growth of isolat L14 and L21.
Key words : growth, temperature, pH, salinity, lactic aid bacteria
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