Characterizing the Three Different Alginate Type of Sargassum siliquosum
This research was aimed to identify the brown seaweed, to characterize the acid, sodium and calcium alginate, and to examine the alginate yield. The identification was done phaenotypically. The extraction method was pretreated by ethanol depigmentation, followed by the extraction of Na2CO3/EDTA and CaCl2 and presipitated with absolute ethanol. The characterization of alginate was done by FT-IR spectroscopy and Thin Layer Chromatography by comparing the samples with standard alginate (Sigma, USA). The key of identification showed that the species was Sargassum siliquosum. There are similarities in signal vibration and TLC spots among the samples and the standard. The TLC test was also showed that those alginates contain mannuronic and guluronic acid. The highest yield was produced by Sodium alginate (40,34% + 0,21), followed by Acid alginate (11,51% + 0,15) and Calcium alginate (4,8% + 0,09).
Keywords: alginate, characterization, Sargassum siliquosum, yield
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Last update: 2021-03-03 12:19:12
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Functional polysaccharides of fucoidan, laminaran and alginate from Malaysian brown seaweeds (Sargassum polycystum, Turbinaria ornata and Padina boryana)
Nur Afifah Mohd Fauziee, Lee Sin Chang, Wan Aida Wan Mustapha, Adibi Rahiman Md Nor, Seng Joe Lim. International Journal of Biological Macromolecules, 127 , 2021. doi: 10.1016/j.ijbiomac.2020.11.067
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