Exploited but Unevaluated: DNA Barcoding Reveals Skates and Stingrays (Chordata, Chondrichthyes) Species Landed in the Indonesian Fish Market

Hawis Madduppa; Rani Utari Ayuningtyas; Beginer Subhan; Dondy Arafat; Prehadi .

doi:10.14710/ik.ijms.21.2.77-84

Exploited but Unevaluated: DNA Barcoding Reveals Skates and Stingrays (Chordata, Chondrichthyes) Species Landed in the Indonesian Fish Market

*Hawis Madduppa - Bogor Agricultural University, Indonesia

Rani Utari Ayuningtyas - Bogor Agricultural University, Indonesia

Beginer Subhan - Bogor Agricultural University, Indonesia

Dondy Arafat - Bogor Agricultural University, Indonesia

Prehadi . - Bogor Agricultural University, Indonesia

Received: 16 Sep 2015; Published: 1 Jun 2016.

DOI: https://doi.org/10.14710/ik.ijms.21.2.77-84 View

BibTex Citation Data :

@article{IK.IJMS9163,
    author = {Hawis Madduppa and Rani Ayuningtyas and Beginer Subhan and Dondy Arafat and Prehadi .},
    title = {Exploited but Unevaluated: DNA Barcoding Reveals Skates and Stingrays (Chordata, Chondrichthyes) Species Landed in the Indonesian Fish Market},
    journal = {ILMU KELAUTAN: Indonesian Journal of Marine Sciences},
  volume = {21},
    number = {2},
    year = {2016},
    keywords = {},
    abstract = {  Reliable and precise species identification is important to fisheries management and conservation. However, many rays and skates in Indonesia are currently being exploited and landed into traditional fish market without a proper identification. Therefore, this study was conducted to identify species of skates and stingrays that were landed and traded in three fish markets in Indonesia (Palabuhanratu, Muara Saban, and Lampung) using molecular techniques and to determine the conservation status of the identified species based on IUCN (International Union for Conservation of Nature and Natural Resources) as well as defined by CITES (Convention on International Trade in Endangered Species). The mitochondrial cytochrome oxidase I (COI) gene was amplified by polymerase chain reaction (PCR) using a pair of primer, fish-BCL and fish-BCH. Of 29 tissue samples collected from the study sites, a total of five species were successfully identified: Dipturus chilensis (4), Himantura walga (1), Neotrygon kuhlii (11), Taeniura lymma (9) and Rhinoptera javanica (4). The Neighbor Joining phylogeny of mitochondrial lineages, based on partial COI gene sequences, the ingroup haplotypes were clustered into five main clades representing each species. The identified stingrays were being listed as vulnerable (D. chilensis and R. javanica), near threatened (H. walga and T. lymma), and data deficient (N. kuhlii) by IUCN, with two species (D. chilensis and H. walga) population were indicated decreased. Unfortunately, all of identified species have not been evaluated by CITES regarding their trade status. As a consequences, a valuable effort should be placed to create a scientific network for monitoring programmes not only on a local scale, and to make pressure on governments for adopting molecular techniques as tools for controlling and avoiding misidentification.            Keywords   : Mitochondrial DNA, Phylogeny, Coral Triangle, Taxonomy, Fisheries  },
   issn = {2406-7598},   pages = {77--84}  doi = {10.14710/ik.ijms.21.2.77-84},
    url = {https://ejournal.undip.ac.id/index.php/ijms/article/view/9163}
}

Citation Format:

Abstract

Reliable and precise species identification is important to fisheries management and conservation. However, many rays and skates in Indonesia are currently being exploited and landed into traditional fish market without a proper identification. Therefore, this study was conducted to identify species of skates and stingrays that were landed and traded in three fish markets in Indonesia (Palabuhanratu, Muara Saban, and Lampung) using molecular techniques and to determine the conservation status of the identified species based on IUCN (International Union for Conservation of Nature and Natural Resources) as well as defined by CITES (Convention on International Trade in Endangered Species). The mitochondrial cytochrome oxidase I (COI) gene was amplified by polymerase chain reaction (PCR) using a pair of primer, fish-BCL and fish-BCH. Of 29 tissue samples collected from the study sites, a total of five species were successfully identified: Dipturus chilensis (4), Himantura walga (1), Neotrygon kuhlii (11), Taeniura lymma (9) and Rhinoptera javanica (4). The Neighbor Joining phylogeny of mitochondrial lineages, based on partial COI gene sequences, the ingroup haplotypes were clustered into five main clades representing each species. The identified stingrays were being listed as vulnerable (D. chilensis and R. javanica), near threatened (H. walga and T. lymma), and data deficient (N. kuhlii) by IUCN, with two species (D. chilensis and H. walga) population were indicated decreased. Unfortunately, all of identified species have not been evaluated by CITES regarding their trade status. As a consequences, a valuable effort should be placed to create a scientific network for monitoring programmes not only on a local scale, and to make pressure on governments for adopting molecular techniques as tools for controlling and avoiding misidentification.

Keywords: Mitochondrial DNA, Phylogeny, Coral Triangle, Taxonomy, Fisheries

Article Metrics:

Article Info

Section: Research Articles

Language: EN

In Vol 21, No 2 (2016): Ilmu Kelautan

Statistics: 1239 1828

Gastropod Density and Diversity in the Mangrove Forest of Pagatan Besar Village, Tanah Laut Regency, Indonesia Measurement of Acoustic Reflection of Tuna Fish Using Echosounder Instrument Utility of Underwater Weenie Life Forms as Voluminous Organisms: A Review Relationship Between Species Composition of Butterflyfishes and Coral Cover (Study case: Taka Bonerate National Park) Dynamic of Particulate Organic Matter and Phytoplankton in Akkeshi-ko Estuary Population Genetics and Phylogeography of Bigeye Tuna in Moluccas Seas, Indonesia

Last update: 2021-01-26 23:08:37

DNA Barcoding and Molecular Phylogeny
B. Akbar John, M. A. Muhamad Asrul, Wahidah Mohd Arshaad, K. C. A. Jalal, Hassan I. Sheikh. 2020. doi: 10.1007/978-3-030-50075-7_8
Population genetic structure of Olive ridley (Lepidochelys olivacea) across Indonesian archipelago revealed by mitochondrial DNA: Implication for management
Regional Studies in Marine Science, 41 , 2021. doi: 10.1016/j.rsma.2020.101600
The use of two pairs primer for CO1 gene amplification on traded stingray at fish auction Tasik Agung Rembang
N Setiati, Partaya, N Hidayah. Journal of Physics: Conference Series, 127 , 2020. doi: 10.1088/1742-6596/1567/3/032056

Last update: 2021-01-26 23:08:38

DNA barcoding of sea turtles (Dermochelyidae and Cheloniidae) and its protocol using different tissues quality: Implication to conservation managers
Madduppa H.H.. IOP Conference Series: Earth and Environmental Science, 127 (1), 2019. doi: 10.1088/1755-1315/278/1/012041
Short communication: Molecular identification of white sea squirt Didemnum sp. (tunicata, ascidiacea) colonies growing over corals in raja ampat Islands, Indonesia
Anzani L.. Biodiversitas, 20 (3), 2019. doi: 10.13057/biodiv/d200304
Short communication: Dna barcoding reveals vulnerable and not evaluated species of sea cucumbers (Holothuroidea and Stichopodidae) from Kepulauan Seribu reefs, Indonesia
Madduppa H.. Biodiversitas, 18 (3), 2017. doi: 10.13057/biodiv/d180305
Short communication: The mitochondrial cytochrome c oxidase subunit i (coi) for identification of batoids collected from landed sites in Medan, Indonesia
Sudibyo M.. Biodiversitas, 21 (11), 2020. doi: 10.13057/biodiv/d211149
DNA barcoding of commercially important reef fishes in Weh Island, Aceh, Indonesia
Fadli N.. PeerJ, 8 , 2020. doi: 10.7717/peerj.9641
Genetic connectivity of the scalloped hammerhead shark Sphyrna lewini across Indonesia and the Western Indian Ocean
Hadi S.. PLoS ONE, 15 (10), 2020. doi: 10.1371/journal.pone.0230763
Morphogenetic and population structure of two species marine bivalve (Ostreidae: Saccostrea cucullata and Crassostrea iredalei) in aceh, Indonesia
Ramadhaniaty M.. Biodiversitas, 19 (3), 2018. doi: 10.13057/biodiv/d190329
Morphometric and DNA barcoding of endemic halmaheran walking shark (Hemiscyllium halmahera, Allen, 2013) in North Maluku, Indonesia
Madduppa H.. Biodiversitas, 21 (7), 2020. doi: 10.13057/biodiv/d210757

Copy this form and after filling it, please send it to ijms@undip.ac.id:

COPYRIGHT TRANSFER STATEMENT

When this article is accepted for publication, its copyright is transferred to ILMU KELAUTAN Indonesian Journal of Marine Sciences, UNDIP. The copyright transfer covers the exclusive right to reproduce and distribute the article, including reprints, translations, photographic reproductions, microform, electronic form (offline, online) or any other reproductions of similar nature.

The author warrants that this article is original and that the author has full power to publish. The author signs for and accepts responsibility for releasing this material on behalf of any and all co-authors. In regard to all kind of plagiarism in this manuscript, if any, only the author(s) will take full responsibility. If the article is based on or part of student’s skripsi, thesis or dissertation, the student needs to sign as his/her agreement that his/her works is going to be published.

Title of article :...........................................................................................................................

Name of Author(s) :...........................................................................................................................

Author’s signature :...........................................................................................................................

Date :...........................................................................................................................

Ilmu Kelautan is published by Marine Science Department, Diponegoro University and Association of Indonesian Coastal Management Experts (HAPPI) under a Creative Commons Attribution-ShareAlike 4.0 International License.