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In vitro lipase enzyme inhibitory activities of green tea and other herbs

1Research Center for Chemistry, Indonesian Institute of Sciences (LIPI), Indonesia

2Research Institute for Tea and Cinchona, Indonesia

Received: 11 Feb 2020; Published: 18 Dec 2020.

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Abstract

Background: Increased lipase activity can increase the amount of monoglycerides and fatty acids absorbed by the body, this is what affects obesity. If pancreatic lipase activity is inhibited, the production of fatty acids will decrease, consequently the level of fat in the blood will also decrease.

Objective: To study anti obesity potential of a single herb or a mixture of green tea and other herbs (Syzygium polyanthum. Lv, Artocarpus communis.Lv, Cinnamomum verum..Lv, Manilkara zapota.Lv, Tectona grandis.Lv) by the in vitro inhibition assay of the lipase enzyme activity

Method: A single (8 herbs) or a mixture of three herbs powder (12 formulas) was put into a tea bag with a total weight of 1.5 g to be brewed in 100 mL bottled mineral water at 70⁰-90⁰C for 10 minutes. In vitro lipase enzyme inhibition assay were conducted to measure the ability of these samples as lipase inhibitor. Orlistat used as positive lipase inhibitor.

Results: There was a difference results of lipase inhibition activity between the sample of a single and a mixture of herbs. Interestingly, the results shows that formula 3 (mixture of green tea, Manilkara zapota and cinnamon). has 53.942 % inhibition, formula 9 (a mixture of green tea, Syzygium polyanthum and cinnamon) has 67.322 % inhibition and formula 12 (a mixture of green tea, Tectona grandis and cinnamon) has 56.612 % inhibition which close to lipase inhibitory activity by standard Orlistat.

Conclusion: The mixture of green tea and two other herbs has a lipase inhibitory activity similar to Orlistat, when compared to the single herb. The highest lipase inhibitory activity is found in formula 9.

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Keywords: green tea; herbs; anti obesity; lipase inhibitor

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  1. Lotulung PDN, Fajriah S, Sundowo A, Filailla E. Anti diabetic Flavanone Compound From The Leaves Of Artocarpus Communis. Indonesian Journal of Chemistry. 2009; 9(3): 466 – 9. DOI: http://dx.doi.org/10.22146/ijc.554
  2. Ranasinghe P, Perera S, Gunatilake M, Abeywardene E, Gunapala N, Premakumara S, Perera K, Lokuhetty D, Katulanda P. Effects of Cinnamomum zeylanicum (Ceylon cinnamon) on blood glucose and lipids in a diabetic and healthy rat model. Pharmacognosy Researches. 2012; 4(2): 73-9. https://doi.org/10.4103/0974-8490.94719
  3. Hidayati MD, Ersam T, Shimizu K, Fatmawati S. Antioxidant Activity of Syzygium polyanthum Extracts. Indonesian Journal of Chemistry. 2017; 17(1): 49-53. https://doi.org/10.22146/ijc.23545
  4. Barbalho SM, Bueno P, Delazari DS, Guiguer EL, Conqueriro DP, Cresoniaraujo A, Souza M, Farinazzi-Machado FMV, Mendes CG, Groppo M. Antidiabetic and Antilipidemic Effects of Manilkara zapota. Journal of Medicinal Food. 2014; 18(3): 385-91. https://doi.org/10.1089/jmf.2013.0170
  5. Ogunmefun OT, E. A. Ekundayo., F. C. Akharaiyi, D. Ewhenodere. Phytochemical screening and antibacterial activities of Tectona grandis L. f. (Teak) leaves on microorganisms isolated from decayed food samples. Tropical plant Research an International Journal. 2017; 4(3): 376-82. https://doi.org/10.22271/tpr.2017.v4.i3.049
  6. Ajisaka. Teh Dahsyat Khasiatnya. Stomata, 2012; vi-viii Surabaya: 1- 28
  7. Johnson R, Bryant S, Alyson L, Huntley. Green Tea and Green Tea Catechin Extracts: An overview of the clinical evidence. Maturitas. 2012; 73(4): 280-7. https://doi.org/10.1016/j.maturitas.2012.08.008
  8. Depkes, 2000. Parameter Standard Umum Ekstrak Tumbuhan Obat, cetakan pertama, Direktorat Jendral Pengawasan Obat dan Makanan, Jakarta
  9. WHO (World Health Organization), 2011. Quality control methods for herbal materials. Malta, Switzerland
  10. Kim YS, Lee YM, Kim H, Kim J, Jang DS, Kim JH, Kim JS. Anti-obesity effect of Morus bombycis root extract: Anti-lipase activity and lipolytic effect. Journal of Ethnopharmacology. 2010; 130(3): 621–4. https://doi.org/10.1016/j.jep.2010.05.053
  11. Standar Nasional Indonesia. 1992. Cara Uji Makanan dan Minuman, SNI 01-2891-1992, point 5.1 and 6.1
  12. Duraipandiyan V, Ignacimuthu S. Antibacterial and antifungal activity of Flindersine isolated from the traditional medicinal plant, Toddalia asiatica (L.) Lam. J Ethnopharmacol. 2009 Jun 25;123(3):494-8. doi: 10.1016/j.jep.2009.02.020
  13. Widyawati PS. Determination of antioxidant capacity in Pluchea indica Less leaves extract and its fractions. International Journal of Pharmacy and Pharmaceutical Sciences. 2016a; 8(9): 32-6. https://dx.doi.org/10.22159/ijpps.2016v8i9.11410
  14. Bhagwat S, Haytowitz D, Holden J. USDA Da-tabase for the Flavonoid Content of Selected Foods). Agricultural Research Service, U.S. De-pament of Agriculture. pp. 2, 98–103. Re-trieved 18 May 2015
  15. Cha KH, Song DG, Kim SM, Pan CH. Inhibition of gastrointestinal lipolysis by green tea, coffee, and gomchui (Ligularia fischeri) tea polyphenols during simulated digestion. J Agric Food Chem. 2012 Jul 25;60(29):7152-7. doi: 10.1021/jf301047f
  16. Megawati, Ernawati T, Meilawati L, Dewijanti ID, Supriadi E. Formulation of Herbal Tea Drinks by Adding Green Tea to Improve Antioxidant Activities. AIP Conference Proceedings. 2019; 2175: 020013-6. https://doi.org/10.1063/1.5134577

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