DOI: https://doi.org/10.14710/jgi.11.1.13-17

The Influence of Aia Tempayang on Interleukin-2 (IL2) Levels on Female Rattus Norvegicus Sprague Dawley Strains in Breast Cancer Prevention

1Department of Nutrition Science, Faculty of Medicine, Universitas Diponegoro, Indonesia

2Faculty of Medicine, Diponegoro University, Indonesia

3Food Technology Program, Department of Agriculture, Faculty of Animal and Agricultural Sciences, Universitas Diponegoro, Indonesia

Received: 29 Mar 2022; Published: 2 Dec 2022.

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Abstract

Background: Breast cancer is a condition in which the cells in the breast tissue grow rapidly and uncontrollably. Risk factors for cancer are those caused by carcinogenic factors, behavior, and diet. Breast cancer causes a decrease in immunity. It can be prevented by consuming high-antioxidant foods such as Aia Tempayang. Aia Tempayang is made from caesalpinia sappan l., scaphium scaphigerum/seeds and ocimum basilicum seeds, each of which contains an antioxidant compound that prevents cancer.

Objectives: To analyze the effect of Aia Tempayang  on the decline of interleukin-2 (il-2) as a deterrent to breast cancer.

Materials and Methods: Female Sprague Dawley was 35 days old n= 30, and divided into five groups: normal control group (K1); control groups induced by DMBA without intervention (K2); treatment group induced by DMBA + caesalpinia sappan l. 0.072 g + scaphium scaphigerum 0.012 g + basilicum seeds 0.045 g (X1); treatment group induced by DMBA + caesalpinia sappan l 0.144 g + scaphium scaphigerum 0.024 g + basilicum seeds 0.09 g (X2); and treatment group induced by DMBA + caesalpinia sappan l 0.288 g + scaphium scaphigerum 0.048 g + basilicum seeds 0.18 g (X3). After 35 days of intervention, serum IL-2 was analyzed using ELISA method. Data analysis used Paired-T Test, One Way ANOVA, and Post-Hoc Bonferroni follow-up test. 

Results: There was a significant difference in serum IL-2 (p=0,000) between supplementation groups found after intervention.The X1, X2, and X3 groups showed decreased of IL-2 to the K2 group without intervention.

Conclusion: Aia tempayang was effective in reducing interleukin-2 levels in the group of mice induced by DMBA for 35 days with doses of  X1, X2 and X3. The dose closest to the normal group is the intervention group X3 with a dose of Caesalpinia sappan l 0.288 g + Scaphium scaphigerum 0.048 g + Basilicum seed 0.18 g.

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Keywords: breast cancer; interleukin-2; caesalpinia sappan l; scaphium scaphigerum; basilicum seeds

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  1. Sun YS, Zhao Z, Yang ZN, et al. Risk factors and preventions of breast cancer. Int J Biol Sci. 2017;13(11):1387-1397. doi: 10.7150/ijbs.21635
  2. Kementerian Kesehatan RI. 2013. Hasil Riset Kesehatan Dasar (Riskesdas) 2013.Jakarta: Badan Penelitian dan Pengembangan Kesehatan Kementerian RI. Available from: http://labdata.litbang.kemkes.go.id/
  3. Lee L, Lee JSC, Waldman SD, et al. Polycyclic aromatic hydrocarbons present in cigarette smoke cause bone loss in an ovariectomized rat model. Bone. 2002;30(6):917-923.doi: 10.1016/s8756-3282(02)00726-3
  4. Swirski, F. K., Nahrendorf, M. Leukocyte behavior in atherosclerosis,myocardial infraction,and heart failure. Science. 2013;339(6116):161-166. doi: 10.1126/science.1230719
  5. Edechi CA, Ikeogu N, Uzonna, et al. Regulation of immunity in breast cancer. Cancers (Basel). 2019;11(8):1–18. doi: 10.3390/cancers11081080
  6. Pol, J. G., Caudana, P., Paillet, J., et al. Effects of interleukin-2 in immunostimulation and immunosuppression. J Exp Med. 2020;217(1):1–15. doi: 10.1084/jem.20191247
  7. Crooke PS, Ritchie MD , Hachey DL, et al. estrogens , enzyme variants , and breast cancer : a risk model. Cancer Epidemiol Biomarkers Prev. 2006;15(9):1620–1629. doi: 10.1158/1055-9965.EPI-06-0198
  8. Yin S, Wei W, Jian F, et al. Therapeutic applications of herbal medicines for cancer patients. Evid Based Complement Alternat Med. 2013;15. doi.org/10.1155/2013/302426
  9. Ye M, Doing Xie W, Lei F et al. Brazilein , an important immunosuppressive component from. Int Immunopharmacol. 2006;6(3):426-432. doi: 10.1016/j.intimp.2005.09.012
  10. Ashiyama MW, Asaki YS, Osokawa TH, et al. Anti-inflammatory constituents of sappan lignum. Biol Pharm Bull. 2009;32(5): 941–944. doi: 10.1248/bpb.32.941
  11. Habli Z, Toumieh G, Fatfat M, et al. Emerging cytotoxic alkaloids in the battle against cancer: overview of molecular mechanisms. Molecules; 2020;22 (2):1–22. doi: 10.3390/molecules22020250
  12. Nasution H N, Ashariati A. Kaheksia kanker dan tatalaksana nutrisi pada penderita kanker. Journal Kedokteran Syiah Kuala. 2021;21(2):189–196. doi: 10.24815/jks.v21i2.19165
  13. Raynard B, Raynard G, Nutritional support of the cancer patient : issues and dilemmas. Crit Rev Oncol Hematol. 2000;34(3):137–168. doi: 10.1016/s1040-8428(00)00048-2
  14. Bozzetti, Arends J, Bachmann P, et al. ESPEN guidelines on nutrition in cancer patients. Clin Nutr. 2010;36(1):148–152. doi: 10.1016/j.clnu.2016.07.015
  15. Ahmadzadeh M, Rosenberg SA. IL-2 administration increases CD4+ CD25 ( Hi) Foxp3+ regulatory T cells in cancer patients. Blood. 2005;107(6):2409–2415. doi: 10.1182/blood-2005-06-2399
  16. Aldi Y, Aria M, Erman, L. Uji efek imunostimulasi ekstrak etanol herba ciplukan (physalis angulata l.) terhadap aktivitas dan kapasitas fagositosis sel makrofag pada mencit putih betina. 2014;4(1):3842
  17. Swarnalatha S, Puratchikody A. Cytokine mediated immunomodulatory properties of kaempferol-5- o - β -d- glucopyranoside from methanol extract of aerial parts of indigofera aspala- thoides vahl ex dc. Int J Res Pharm Sci. 2014;5(1):73–78
  18. Aspord C, Gonzales AP, Gallegos M, et al. Breast cancer instructs dendritic cells to prime interleukin 13 – secreting CD4 + T cells that facilitate tumor development The Journal of Experimental Medicine. J Exp Med. 2007;204(5):1037–1047. doi: 10.1084/jem.20061120
  19. Fudholi A, Meiyanto E, Donatus I A, et al. Efek penghambatan terhadap pertumbuhan tumor paru dan uji ketoksikan akut ekstrak kapsul chang sheuw tian ran ling yao pada mencit (Mus Musculus) dan tikus ( Ratus Tanezumi ). Jbi. 2002; 5(1): 1–21. doi: 10.14203/jbi.v5i1.3202
  20. Lago J, Allesandra C, Maircia M, et al. structure-activity association of flavonoids in lung diseases. Molecules. 2014; 19 (3): 3570–3595. doi: 10.3390/molecules19033570

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