1Guru SMA Negeri 2 Kabupaten Pati, Indonesia
2Bagian Biokimia, Fakultas Kedokteran, Universitas Diponegoro, Semarang, Indonesia
3Fakultas Kedokteran, Universitas Diponegoro, Semarang, Indonesia
BibTex Citation Data :
@article{JGI8612, author = {Iin Tusanti and Andrew Johan and RA Kisdjamiatun}, title = {Sitotoksisitas in vitro ekstrak etanolik buah parijoto (Medinilla speciosa, reinw.ex bl.) terhadap sel kanker payudara T47D}, journal = {Jurnal Gizi Indonesia (The Indonesian Journal of Nutrition)}, volume = {2}, number = {2}, year = {2014}, keywords = {fruit, Medinilla speciosa (Reinw.ex Bl.), cytotoxixity, MTT assay, breast cancer, T47D cell line}, abstract = { Background : Several studies focused on phytochemical as agents of cancer prevention and co-chemotherapy. One of Indonesian plant which has edible fruit but it hasn’t been completely explored is Medinilla speciosa (Reinw.ex Bl.). Objective : The aim of this study is to examine the cytotoxic activity (IC 50 value) of Medinilla speciosa (Reinw.ex Bl.) fruit ethanolic extract. Methods : Medinilla speciosa (Reinw.ex Bl.) fruit ethanolic extract was used in this study. The cytotoxic activity was investigated in vitro on human breast cancer T47D cell-line. The cells viability were assessed using MTT colorimetric assay. Breast cancer T47D cell lines was treated with fruit ethanolic extract (10, 25, 50, 100, 250, 500 and 1000 µg/ml) for 24 hour of incubation. This study also identified phytochemical compound of the fruit with thin layer chromatography (TLC). Results : The result showed that ethanolic extract of Medinilla speciosa (Reinw.ex Bl.) has moderate cytotoxicity on breast cancer T47D cell line with IC 50 value of 614.50 µg/ml and yield the decrease of cell viability at higher concentration. Medinilla speciosa fruit can not be used as anticancer agent but chemoprevention agent. Phytochemical test showed that the fruit extract contain flavonoid and saponin compound. Conclusion : Ethanolic extract of Medinilla speciosa fruit exhibited moderate cytotocicity on breast cancer T47D cell lines with IC 50 value was 614,50 µg/ml thus it can be used as chemopreventioan agent. }, issn = {2338-3119}, pages = {53--58} doi = {10.14710/jgi.2.2.53-58}, url = {https://ejournal.undip.ac.id/index.php/jgi/article/view/8612} }
Refworks Citation Data :
Background: Several studies focused on phytochemical as agents of cancer prevention and co-chemotherapy. One of Indonesian plant which has edible fruit but it hasn’t been completely explored is Medinilla speciosa (Reinw.ex Bl.). Objective : The aim of this study is to examine the cytotoxic activity (IC50 value) of Medinilla speciosa (Reinw.ex Bl.) fruit ethanolic extract.
Methods : Medinilla speciosa (Reinw.ex Bl.) fruit ethanolic extract was used in this study. The cytotoxic activity was investigated in vitro on human breast cancer T47D cell-line. The cells viability were assessed using MTT colorimetric assay. Breast cancer T47D cell lines was treated with fruit ethanolic extract (10, 25, 50, 100, 250, 500 and 1000 µg/ml) for 24 hour of incubation. This study also identified phytochemical compound of the fruit with thin layer chromatography (TLC).
Results: The result showed that ethanolic extract of Medinilla speciosa (Reinw.ex Bl.) has moderate cytotoxicity on breast cancer T47D cell line with IC50 value of 614.50 µg/ml and yield the decrease of cell viability at higher concentration. Medinilla speciosa fruit can not be used as anticancer agent but chemoprevention agent. Phytochemical test showed that the fruit extract contain flavonoid and saponin compound.
Conclusion: Ethanolic extract of Medinilla speciosa fruit exhibited moderate cytotocicity on breast cancer T47D cell lines with IC50 value was 614,50 µg/ml thus it can be used as chemopreventioan agent.
Article Metrics:
Last update:
Antifungal activities of Medinilla speciosa Blume fruit extracts against Candida albicans and Trichophyton rubrum
Isolation and Identification of Phytoconstituens from Methanol Extract Parijoto (Medinilla speciosa)
Anticancer potential of turmeric (Curcuma longa) ethanol extract and prediction of its mechanism through the Akt1 pathway
Cytotoxicity effect of nonpolar extract from parijoto (medinilla speciosa reinw. ex. bl) fruit against hela and widr cell line
Antihyperlipidemic and anti-obesity effects of the methanolic extract of parijoto (Medinilla speciosa)
Last update: 2024-11-20 12:07:58
Analysis of lipid profile and atherogenic index in hyperlipidemic rat (Rattus norvegicus Berkenhout, 1769) that given the methanolic extract of Parijoto (Medinilla speciosa)
Jurnal Gizi Indonesia (The Indonesian Journal of Nutrition) is published under licensed of a CC BY-SA Creative Commons Attribution-ShareAlike 4.0 International LicenseThe Authors submitting a manuscript do so on the understanding that if accepted for publication, copyright of the article shall be assigned to Jurnal Gizi Indonesia (The Indonesian Journal of Nutrition) and Department of Nutrition Science, Faculty of Medicine, Universitas Diponegoro as publisher of the journal.
Copyright encompasses exclusive rights to reproduce and deliver the article in all form and media, including reprints, photographs, microfilms and any other similar reproductions, as well as translations. The reproduction of any part of this journal, its storage in databases and its transmission by any form or media, such as electronic, electrostatic and mechanical copies, photocopies, recordings, magnetic media, etc., will be allowed only with a written permission from Jurnal Gizi Indonesia (The Indonesian Journal of Nutrition).
Jurnal Gizi Indonesia (The Indonesian Journal of Nutrition), Faculty of Medicine, Universitas Diponegoro, the Editors and the Advisory Editorial Board make every effort to ensure that no wrong or misleading data, opinions or statements be published in the journal. In any way, the contents of the articles and advertisements published in the Indonesian Journal of Nutrition are sole and exclusive responsibility of their respective authors and advertisers.
View JGI Stats
