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Determination of the best method for processing gambier liquid by-product [Uncaria gambir (hunter) roxb] as natural antioxidant sources

A. S. Ismail orcid  -  Post Graduate Student, Faculty of Animal Science, Andalas University, Indonesia
*Y. Rizal orcid  -  Department of Animal Nutrition, Faculty of Animal Science, Andalas University, Indonesia
A. Armenia orcid  -  Department of Pharmacology, Faculty of Pharmacy, Andalas University, Indonesia
A. Kasim orcid  -  Departmen of Crops and Technology, Faculty of Agriculture Technology, Andalas University, Indonesia
Open Access Copyright (c) 2021 Journal of the Indonesian Tropical Animal Agriculture

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Abstract
The study was conducted to determine the best gambier liquid by-product (GLB) processing methods based on antioxidant activity as natural antioxidant sources. The processing methods were A (ethyl acetate fractionation), B (oven drying) and C (freeze drying) methods. The observed variables were dry extract (using weighing method), tannin (using hide powder method), catechin (using SNI 01- 339-2000 method), total phenol (using folin ciocalteu reagents) and antioxidant activities (DPPH method) based on ICAO50. These antioxidant activities were compared with vitamin C as a positive control. The results indicated that the different processing methods significantly affected (P<0.05) dry extract, tannin, catechin, total phenol and antioxidant activity of GLB. The highest dry extract, tannin, catechin, total phenol, and antioxidant activity from GLB were at treatment B (10.76%), B (39.69%), A (86.07%), C (80.97 mg GAE/g), and C (ICAO50 2.74 µg/mL), respectively. The antioxidant activity on treatment C was not different from vitamin C antioxidant activity (2.88µg/mL). Thus, the treatment C (freeze drying method) was the best method to process GLB based on antioxidant activity as natural antioxidant sources.

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Keywords: antioxidant-activity; chemical content; drying method; ethyl acetate fractionation; gambier liquid by-product.
Funding: The Ministry of Research, Technology and Higher Education of the Republic of Indonesia

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