PURIFICATION OF ANGIOTENSIN CONVERTING ENZYME INHIBITORY PEPTIDE DERIVED FROM KACANG GOAT MEAT PROTEIN HYDROLYSATE

*J. Jamhari  -  Faculty of Animal Science, Gadjah Mada University,, Indonesia
L.M. Yusiati  -  Faculty of Animal Science, Gadjah Mada University,, Indonesia
E. Suryanto  -  Faculty of Animal Science, Gadjah Mada University,, Indonesia
M.N. Cahyanto  -  Faculty of Agricultural Technology, Gadjah Mada University,, Indonesia
Y. Erwanto  -  Faculty of Animal Science, Gadjah Mada University,, Indonesia
M. Muguruma  -  Faculty of Agriculture, University of Miyazaki,, Japan
Published: 15 Dec 2013.
Open Access
Citation Format:
Abstract
The objective of this study was to identify the Angiotensin Converting Enzyme (ACE) inhibitorypeptide derived from Kacang goat meat protein hydrolysate. Kacang goat meat loin section washydrolyzed with pepsin, trypsin and chymotrypsin. Protein hydrolysate of Kacang goat meat was thentested the protein concentration and ACE inhibitory activity. ACE inhibitory peptide of the proteinhydrolysate was purified through several steps of purification by column SEP-PAK Plus C18 Cartridgeand RP-HPLC using a Cosmosil column 5PE-SM, 4.6 x 250　mm. The sequence of amino acid of ACEinhibitory peptide was identified by amino acid sequencer. The results showed that amino acidssequence of ACE inhibitory peptide derived from protein hydrolysate of Kacang goat meat was leu-thrglu-ala-pro-leu-asn-pro-lys-ala-arg- asn-glu-lys. It had a molecular weight (MW) of 1581 and occurredat the position of 20th to 33rd residues of b-actin of goat meat protein (Capra hircus). The ACE inhibitoryactivity (IC50) of the peptide was 190 mg/mL or 120 mM.
Keywords: ACE Inhibitory peptide. meat protein hydrolysate. Kacang goat

Article Metrics:

Article Info
Section: Articles
Language: EN
In Vol 38, No 4 (2013): (December)
Statistics: 1203 885
Others articles
Analysis of the local resource-based dairy cattle development in Central Java A CASE STUDY OF LUNTIAN MULTI-PURPOSE COOPERATIVE IN BARANGAY LALAIG, TIAONG, QUEZON, PHILIPPINES: A VERTICAL INTEGRATION APPROACH THE EFFECTS OF HERBS ON MILK YIELD AND MILK QUALITY OF MASTITIS DAIRY COW BUSINESS PERFORMANCE OF DUCK FARMERS IN BREBES REGENCYCENTRAL JAVA EFFECT OF TURMERIC EXTRACT ON BLOOD PARAMETERS, FEED EFFICIENCY AND ABDOMINAL FAT CONTENT IN BROILERS THE ROLE OF TURMERIC POWDER IN LIPID METABOLISM AND ITS EFFECT ON QUALITY OF THE FIRST QUAIL’S EGG

Last update: 2021-02-23 23:44:39

  1. Increased Inhibition of Angiotensin Converting Enzyme (ACE) Obtained from Indonesian Buffalo Meat Protein Using SEP-PAK Plus C18

    Pakistan Journal of Nutrition, 17 (9), 2018. doi: 10.3923/pjn.2018.434.440

  2. Angiotensin-converting enzyme inhibitor activity of peptides derived from Kacang goat skin collagen through thermolysin hydrolysis

    Veterinary World, 14 (1), 2021. doi: 10.14202/vetworld.2021.161-167

Last update: 2021-02-23 23:44:41

  1. Hydrolyzation of duck meat protein using Bacillus cereus TD5B protease, pepsin, trypsin and their potency as an angiotensin converting enzyme inhibitor

    Winarti A.. Journal of the Indonesian Tropical Animal Agriculture, 44 (3), 2019. doi: 10.14710/jitaa.44.3.266-276

  2. Angiotensin-converting enzyme inhibitor activity of peptides derived from Kacang goat skin collagen through thermolysin hydrolysis

    Veterinary World, 14 (1), 2021. doi: 10.14202/vetworld.2021.161-167

  3. Increased inhibition of angiotensin converting enzyme (ACE) obtained from Indonesian buffalo meat protein using SEP-PAK plus C18

    Sujarwanta R.. Pakistan Journal of Nutrition, 17 (9), 2018. doi: 10.3923/pjn.2018.434.440