Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University, Indonesia
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@article{JKSA18119, author = {Reny Selvia and Wuryanti Wuryanti and Sriatun Sriatun}, title = {Isolasi dan Karakterisasi Kitinase dari Isolat Jamur Akuatik Kitinolitik berasal dari Kupu-kupu (Lepidoptera)}, journal = {Jurnal Kimia Sains dan Aplikasi}, volume = {16}, number = {3}, year = {2013}, keywords = {kitinase; jamur akuatik kitinolitik; Ueda Arai; Lowry}, abstract = {Telah dilakukan penelitian tentang isolasi dan karakterisasi kitinase dari isolat jamur akuatik kitinolitik berasal dari kupu-kupu ( Lepidoptera ). Penelitian ini bertujuan untuk memperoleh kitinase dan data aktivitas spesifik kitinase dari isolat jamur akuatik kitinolitik yang berasal dari kupu-kupu serta memperoleh data kondisi optimum kitinase meliputi pH dan suhu. Pengujian aktivitas kitinase pada penelitian ini dilakukan dengan metode Ueda Arai, sedangkan pengukuran kadar protein dilakukan dengan metode Lowry. Aktivitas spesifik pada penelitian ini diperoleh dari rasio antara unit aktivitas dengan kadar protein kitinase. Aktivitas spesifik tertinggi kitinase pada penelitian ini ditunjukkan oleh fraksi 3 yaitu sebesar 51,17 unit/mg protein. Hasil karakterisasi kitinase menunjukkan bahwa pH 3,8 dan suhu 40,5 o C merupakan kondisi optimum kitinase.}, issn = {2597-9914}, pages = {97--101} doi = {10.14710/jksa.16.3.97-101}, url = {https://ejournal.undip.ac.id/index.php/ksa/article/view/18119} }
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