1Poltekkes Kemenkes Palembang, Indonesia
2Department of Biology, Faculty Mathematics and Natural Sciences, Universitas Sriwijaya, Indonesia
3Department of Biochemistry, Faculty of Medicine, Universitas Sriwijaya, Indonesia
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@article{JKSA24539, author = {Sri Harianja and Salni Salni and Subandrate Subandrate}, title = {Cytotoxic, Antiproliferation, and Necrosis Effects of the n-Hexane Fraction Extract of Gendola Leaf (Basella rubra Linn.)}, journal = {Jurnal Kimia Sains dan Aplikasi}, volume = {23}, number = {1}, year = {2020}, keywords = {cytotoxic; antiproliferation; apoptosis; Gendola leaf extract and fraction (Basella rubra Linn.); necrosis}, abstract = {Breast cancer is the second most common cancer in women. Several studies have been conducted on natural materials to see the ability of its activity as a new anticancer compound. The purpose of this study was to determine the cytotoxic activity of Gendola leaf ( Basella rubra Linn. ) extracts and fractions, in inhibiting proliferation and inducing apoptosis of T47D breast cancer cells. The design of this study was experimental laboratory research using the in-vitro method. Cytotoxic and anti-proliferation tests on T47D cancer were using the MTT method, and the apoptosis test was using the flow cytometry method. Cytotoxic of extracts and fractions of n-hexane Gendola leaves were tested with a concentration series of 1000; 500; 250; 125; 62.5 μg/mL and doxorubicin was studied a series concentration of 0.5; 0.25; 0.125; 0.0625; 0.03125 μg/mL for 24 hours. Antiproliferation test used n-hexane fraction with a concentration series of 1IC 50 , ½IC 50 , ¼IC 50 , and ⅛IC 50 with an incubation time of 24 and 48 hours. Apoptosis test utilized n-hexane fraction with a concentration series of 1IC 50 , ½IC 50 with an incubation time of 24 hours. The results showed that the ethanol extract had IC 50 424 μg/mL, n-hexane fraction 292 μg/mL. The n-hexane fraction had an anti-proliferation effect at a concentration of 1IC 50 within 32 hours but was unable to induce T47D cell apoptosis. These results indicate that the n-hexane fraction of Gendola leaf has a cytotoxic effect, which can inhibit proliferation, that is unable to induce apoptosis but induce necrosis of T47D breast cancer cells.}, issn = {2597-9914}, pages = {8--13} doi = {10.14710/jksa.23.1.8-13}, url = {https://ejournal.undip.ac.id/index.php/ksa/article/view/24539} }
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