1Chemical Study Program, Faculty of Science, Institute Technology of Sumatera, Jl Terusan Ryacudu, WayHui, Lampung Selatan, 35365, Lampung, Indonesia
2Post Graduate Program of Biotechnology, Post Graduate Program, Universitas Gadjah Mada, Jl Teknika Utara, Catur Tunggal, Sleman, 55281, Yogyakarta, Indonesia
3Faculty of Veterinary Medicine, Universitas Gadjah Mada, Jl Fauna No 2, Karang gayam, Catur tunggal, Sleman, 55281, Yogyakarta, Indonesia
4 Department of Agriculture Microbiology, Faculty of Agriculture, Universitas Gadjah Mada, Karang malang, Catur Tunggal, Sleman, 55281, Yogyakarta, Indonesia
BibTex Citation Data :
@article{JKSA59357, author = {Irwan Sudarmanto and Wayan Tunas Artama and Muhammad Saifur Rohman}, title = {Physicochemical Characterization and Comparative Analysis of Ribulose 1,5 Biphosphate Carboxylase-Oxygenase Like Proteins (RLP) from Halophilic Chromohalobacter salexigens BKL 5 and Non-Halophilic Counterparts Using in Silico Approaches}, journal = {Jurnal Kimia Sains dan Aplikasi}, volume = {27}, number = {4}, year = {2024}, keywords = {halophilic; metaboanalyst 5.0; Chromohalobacter salexigens BKL5; RuBisCo-like Protein; Partial Least Square}, abstract = { This study is intended to analyze and compare halophilic RuBisCo-like proteins (RLP) with non-halophilic homologs using computational analysis approaches. The data used were protein sequences from NCBI and protein sequences from clonned and expressed RuBisCo-like proteins Chromohalobacter salexigens BKL 5 from our previous study. The analysis was in the form of Principal Component Analysis (PCA) and Partial Least Square (PLS). The tools used were Origin Lab Full Version 2019 and Metaboanalyst 5.0. The parameters tested were isoelectric point (pI), negative charge (acidic amino acids), aliphatic index, and GRAVY index using the ProtParam tool from Expasy. Calculations analysis showed that the amino acid residues in halophilic RuBisCo-like proteins that differ significantly from their homologs were glutamic acid and alanine, while the variables that could be observed as differentiating were negative charge and aliphatic index. Analysis shows that some parameters obtained from the program can be used as discriminants to differentiate halophilic and non-halophilic RuBisCo-like proteins. }, issn = {2597-9914}, pages = {151--159} doi = {10.14710/jksa.27.4.151-159}, url = {https://ejournal.undip.ac.id/index.php/ksa/article/view/59357} }
Refworks Citation Data :
This study is intended to analyze and compare halophilic RuBisCo-like proteins (RLP) with non-halophilic homologs using computational analysis approaches. The data used were protein sequences from NCBI and protein sequences from clonned and expressed RuBisCo-like proteins Chromohalobacter salexigens BKL 5 from our previous study. The analysis was in the form of Principal Component Analysis (PCA) and Partial Least Square (PLS). The tools used were Origin Lab Full Version 2019 and Metaboanalyst 5.0. The parameters tested were isoelectric point (pI), negative charge (acidic amino acids), aliphatic index, and GRAVY index using the ProtParam tool from Expasy. Calculations analysis showed that the amino acid residues in halophilic RuBisCo-like proteins that differ significantly from their homologs were glutamic acid and alanine, while the variables that could be observed as differentiating were negative charge and aliphatic index. Analysis shows that some parameters obtained from the program can be used as discriminants to differentiate halophilic and non-halophilic RuBisCo-like proteins.
Article Metrics:
Last update:
Last update: 2026-09-21 05:34:20
As an article writer, the author has the right to use their article for various purposes, including use by institutions that employ the author or institutions that provide funding for the research. These author rights are granted without special permission.
Authors who publish a paper in JKSA have broad rights to use their work for teaching and scientific purposes without the need to request permission, including: (i) teaching in the author's class or institution; (ii) presenting the work at meetings or conferences and distributing copies to participants; (iii) using the work in training conducted by the author or the author's institution; (iv) distributing copies to colleagues for research purposes; (v) using the work in the compilation of subsequent works by the author; (vi) including the work in a thesis or dissertation; (vii) reusing part of the article in another work, with appropriate citation; (viii) preparing derivative works, with appropriate citation; and (ix) voluntarily posting the work on open websites operated by the author or the author's institution for scientific purposes, in accordance with the CC BY-SA License.
Authors and readers may copy and redistribute the material in any medium or format, and may remix, transform, and build upon the material for any purpose, provided that they give appropriate credit by citing the article or its content, provide a link to the license, and indicate if changes were made.
The authors submitting a manuscript do so on the understanding that, if accepted for publication, the copyright of the article shall be assigned to Jurnal Kimia Sains dan Aplikasi (JKSA). Copyright encompasses the rights to reproduce and distribute the article in all forms and media, including reprints, photographs, microfilms, and any other similar reproductions, as well as translations.
Reproducing any part of this journal, storing it in a database, or transmitting it in any form or medium is permitted without written permission from JKSA. However, the original source must be properly cited in accordance with academic conventions.
JKSA, the Chemistry Department of Diponegoro University, and the Editor make every effort to ensure that no inaccurate, false, or misleading data, opinions, or statements are published in JKSA. However, the content of each article is the sole and exclusive responsibility of its authors.
The Copyright Transfer Form can be downloaded here: [Copyright Transfer Form - Indonesian] [Copyright Transfer Form - English]. The copyright form should bear the authors' original signatures and be sent to the Editor as a printed document, a scanned document sent via email, or by fax.
Prof. Adi Darmawan, Ph.D (Editor in Chief)
Editor in chief of Jurnal Kimia Sains dan Aplikasi (JKSA)
Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University
Visitor: View My Stats
Jurnal Kimia Sains dan Aplikasi is indexed in:
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
