Efek Caffeine Terhadap Jumlah Sel Inflamasi pada Penyembuhan Luka Skin Graft pada Tikus Sprague Dawley

*Wahyu Haris Prabowo -  Mahasiswa Program Studi S2 Ilmu Biomedik, Fakultas Kedokteran, Universitas Diponegoro, Indonesia
Received: 15 May 2019; Published: 15 May 2019.
Open Access Copyright 2019 MEDIA KESEHATAN MASYARAKAT INDONESIA
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Citation Format:
Article Info
Section: Research article
Language: ID
Full Text:
Statistics: 12 4
Abstract
ABSTRAK
Latar Belakang: Skin graft terapi pilihan pada proses penyembuhan luka yang berkembang pesat. Sistem imunitas berperan dalam penyembuhan luka skin graft. Kopi mengandung caffeine (1,3,7-trimethylxanthine) sebagai antioksidan berperan dalam penyembuhan luka melalui sistem imun. Tujuan penelitian ini membuktikan efek caffeine berbagai dosis dalam meningkatkan jumlah sel inflamasi pada luka skin graft.
Metode: Studi eksperimental dengan “Blinded randomized post test only controlled group design”. Seluruh sampel (Tikus Sprague Dawley) dilakukan Skin Graft Autologus pada waktu yang bersamaan. Sampel dibagi secara acak menjadi 4 grup (K= Tanpa intake caffeine), (P1= Caffeine 3 mg), (P2 = Caffeine 6 mg), (P3 = Caffeine 9 mg). Perhitungan jumlah sel makrofag jaringan, sel neutrofil dan monosit darah tepi dilakukan pada hari ke 7 pasca Skin Graft. Data dianalisis dengan metode ANOVA, Kruskall-Wallis dan Mann-Whitney Test.
Hasil: Perbandingan jumlah makrofag jaringan didapatkan perbedaan yang bermakna antara kelompok K dengan P2 (p = 0,011), K dengan P3 (p = 0,008); P1 dengan P2 (p = 0,009); P1 dengan P3 (p = 0,006); dan P2 dengan P3 (p = 0,008). Perbedaan tidak bermakna didapatkan antara kelompok K dengan P1 (p = 0,343). Pada hasil uji neutrofil darah tepi, uji Kruskal Wallis didapatkan nilai p = 0,961, sehingga tidak didapatkan perbedaan bermakna. Pada hasil uji monosit, Hasil uji One Way ANOVA didapatkan nilai p = 0,160, tidak ada perbedaan bermakna jumlah monosit darah tepi pada keempat kelompok.
Simpulan: Terdapat perbedaan bermakna jumlah sel makrofag jaringan pada penggunaan caffeine dosis bertingkat proses penyembuhan luka skin graft autologus tikus Sprague Dawley.
Keywords
Sel inflamasi, skin graft, caffeine

Article Metrics:

  1. Departemen Kesehatan Republik Indonesia. Laporan Hasil Riset Kesehatan Dasar Indonesia (Riskesdas) 2013 Bidang Cedera. In: Indonesia DK, editor. Jakarta: Badan Litbangkes Departemen Kesehatan RI; 2015.
  2. Riyadina W. Pola dan Determinan Cedera di Indonesia. In: Indonesia DKR, editor. Jakarta: Laporan hasil analisis lanjut Riskesdas, Pusat Penelitian dan Pengembangan Biomedis dan Farmasi; 2013.
  3. Wardhana A, Basuki A. The epidemiology of burn in Indonesia's national referral burn center from 2013 to 2015. Burns. 2017;142(1): 67-73.
  4. Syamsuhidayat R, Theddeus P, Jong WD, Riwanto I, Rudiman R. Buku ajar ilmu bedah. Jakarta.2017.EGC: 97 - 114 p.
  5. Mann MW, Berk D, Popkin DL, Bayliss SJ. Handbook of dermatology a practical manual. England: Oxford Blackwell Publishing; 2009.
  6. Shrycock JC, Belardinelli L. Adenosine and a adenosine receptors in the cardiovascular system: Biochemistry, Physiology and Pharmacology. Am J of Cardiol. 2007;79:2 - 10.
  7. Valls MD, Cronstein B, Montesinos MC. Adenosine receptors agonist for promotion of dermal wound healing. Biochemistry Pharmacology. 2009;77:1117 - 24.
  8. Motegi T, Katayama M, Uzuka Y, Okamura Y. Evaluation of anticancer effects and enhanced doxorubicyn cytotoxicity of xanthine derivatives using canine hemangiosarcoma cell lines. Res Vet Science. 2013;95:600 - 5.
  9. Cronstein BN. Adenosine receptors and fibrosis: A translational review. Biol Rep. 2011;3(21):1 - 12
  10. Einfield GP, Leibovich SJ. Macrophage heterogenity and wound healing. New Jersey: Mary Ann Liebert Inc; 2011.
  11. Koh TJ, DiPietro LA. Inflammation and wound healing: the role of the macrophage. Expert Rev Mol Med. 2011;13:e23.
  12. Brancato SK, Albina JE. Wound macrophages as key regulators of repair: origin, phenotype, and function. Am J Pathol. 2011;178(1):19-25.
  13. Bonyanian ZMR, Roselyn B. Caffeine and it’s potential role in attenuating impaired wound healing. Journal of Caffeine Research. 2016;5(4):141 - 8.
  14. Li J, Li G, Hu JL,Fu XH, Zeng YJ, Zhou YG, Xiong G. Chronic or high dose acute caffeine treatment protects mice against oleic acid-induced acute lung injury via an adenosine A2A receptor-independent mechanism. Eur J Pharmacol. 2011;654(3):295 - 303.
  15. Feoktistov I, Goldstein AE, Ryzhov S, Zeng D, Belardinelli L. Differential expression of adenosine receptors in human endothelial cells; role of A2B receptors in angiogenic factor regulation. Circ Res. 2012;90:531 - 8.
  16. Leibovich SJ, Chen J, Einfeld P, Elson G, Rosania A. Synergistic up-regulation of vascular endothelial growth factor expression in murine macrophages by adenosine A2A receptor agonist and endotoxin. Am J of Pathol. 2002;160:2231 - 44.
  17. Ojeh N, Stojadinovic O, Pastar I, Sawaya A. The effects of caffeine on wound healing. Inter Wound J. 2014;3(5):605 - 13.
  18. Diaz MM, Salin PR. Purine molecule as hypnogenic factors role of adenosine, ATP and caffeine. Central Nervous System Agents Medical Chemistry. 2010;10:259 - 68.
  19. Silverberg J, Patel M, Brody N, Jagdeo J. Caffeine protects human skin fibroblast from acute reactive oxygen species – induced necrosis. Journal Drugs Dermatology. 2012;11:1342 - 6
  20. Prasetyono T. General concept of wound healing. Med J of Indonesia. 2009;18:208 - 16.
  21. Maria C, Renato D, Luciano O. Influence of immunonutritional supplementation on skin wound healing in rats. Bras Cir Plast. 2014;29(3):432 – 8
  22. Barcelos RP, Souza M, Amaral GP, Stefanello ST. Caffeine intake may modulate inflammation markers in trained rats. Nutrients. 2014;6:1678 - 90.
  23. Yuwono HS. The new paradigm of wound management using coffee powder. J Surg. 2014;2:25 - 9.
  24. Li J, Li G, Hu JL,Fu XH, Zeng YJ, Zhou YG, Xiong G. Chronic or high dose acute caffeine treatment protects mice against oleic acid-induced acute lung injury via an adenosine A2A receptor-independent mechanism. Eur J Pharmacol. 2011;654(3):295 - 303.
  25. Sarobo C, Lacorte LM, Martins M, Rinaldi JC, Moroz A, Scarano WR, et al. Chronic caffeine intake increases androgenic stimuli, epithelial cell proliferation and hyperplasia in rat ventral prostate. Int J Exp Pathol. 2012;93(6):429-37.
  26. Martinez FO, Helming L, Gordon S. Alternative activation of macrophages: an immunologic functional perspective. Annu Rev Immunol. 2009;27:451-83.
  27. Da-Rocha LF, Macedo SJ, Luiz CM, Santos ARS. Pharmacology of adenosine receptors and their signalling role in immunity and inflammation. Pharm and Therapeutic J. 2014;3:1620 - 2.
  28. Grande D. Skin grafting. Am J of Pathol. 2006;4(11):3321 - 6.
  29. Laplante AF, Germain L, Auger FA, Moulin V. Mechanisms of wound reepithelization: hints from a tissue engineered reconstructed skin to long-standing questions. FASEB J. 2011;15:2377 - 89.
  30. Barbul A, Oliver C. Immunonutrition: role in wound healing and tissue regeneration. Adv in Wound Care J. 2014;3(1):46 - 53.
  31. Barrientos S, Stojadinovic O, Golinko MS, Brem H. Growth factors and cytokines in wound healing. Wound Repair Regeneration. 2008;16:585 - 601
  32. Su SH, Shu HW, Chen KM, Yeh H, Su SJ. Caffeine inhibits adipogenic differentiation of primary adipose – derived stem cells and bone marrow stromal cells. Journal of Toxicology In Vitro. 2013;27:1830 - 7
  33. Hashimoto T, Schmidt P, Yang CS, Dong Z. Caffeine inhibits cell proliferation by G0/G1 phase arrest in JB6 cells. Cancer Res. 2004;64:344 - 9.
  34. Kumar V, Abbas AK, Fausto N, Aster JC, Robbins, Cotran. Pathologic basic of disease. Philadelphia USA: Elsevier Health Sciences; 2009. 185 - 210 p.