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Submitted: 04-07-2017
Published: 24-08-2018
Section: Research Article
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Pretreated of sugarcane bagasse was used as a carrier for immobilization of Saccharomyces cerevisiae. Pretreatments were carried out by steaming, pressurized steam, and  combination both of procedure.  The objectives of  this research was to investigate the effect of pretreatment on sugarcane bagasse to cells adsorption and bioethanol production.  Immobilization process was conducted in a ratio of 2.5 g carrier/50 mL cell suspension.  Whole cell biocatalyst as much as 1% (w/v) was used as inoculum for bioethanol fermentation.  The best pretreated sugarcane bagasse for carrier of immobilized cells was obtained using steam treatment for 30 minutes.  Those treatment improved the physical properties of carrier, increased the cell retention up to 10.05 mg/g.  The use of whole cell biocatalyst after steaming pretreatment enhanced ethanol yield 1.5 times higher than control.

Keywords: bioethanol; immobilization; pretreatment; steam treatment; sugarcane bagasse


treatment; fermentation; biocatalyst; biomass conversion

  1. Sita Heris Anita 
    Indonesian Institute of Sciences, Indonesia
    Research Center for Biomaterials
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