ULTRASONIC-ASSISTED EXTRACTION OF ANTIOXIDANT PHENOLIC COUMPOUNDS FROM EUCHEUMA COTTONII

Dyah Hesti Wardhani; Denni Kartika Sari; Aji Prasetyaningrum

doi:10.14710/reaktor.14.4.291-297

DOI: https://doi.org/10.14710/reaktor.14.4.291-297

ULTRASONIC-ASSISTED EXTRACTION OF ANTIOXIDANT PHENOLIC COUMPOUNDS FROM EUCHEUMA COTTONII

*Dyah Hesti Wardhani - Department of Chemical Engineering-Diponegoro University Semarang, Indonesia

Denni Kartika Sari - Department of Chemical Engineering-Tirtayasa University Banten, Indonesia

Aji Prasetyaningrum - Department of Chemical Engineering-Diponegoro University Semarang, Indonesia

Published: 9 Dec 2014.

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Abstract

The influences of ultrasonic-assisted extraction (UAE) conditions on phenolic compounds of Eucheuma cottonii seaweeed were investigated. The effects of temperature, time and liquid-solid ratio were evaluated based on the total phenolic content (TPC). The result showed a significant variability of effect of temperature, time, and liquid-solid ratio on TPC. Application of ultrasound reduced the optimum temperature, time and liquid-solid ratio of the extraction when compared to the conventional extraction. The highest phenolic content (4.44 mg gallic acid equivalent/g dry weight) was obtained at 3:1(v/w) of liquid-solid ratio, 55^oC of temperature and 35 min of extraction time. At this condition, the extract showed ability to scavenging 91% of 1,1-diphenyl 2 picrylhydrazyl (DPPH radical) and 10.9% of H₂O₂. High ability of the extract to scavenge DPPH radical but low in scavenging H₂O₂indicated that the extract dominated by primary antioxidant compounds which work by donating hydrogen atoms to bind free radical.

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Keywords: antioxidant; DPPH radical; Eucheuma cottonii; H2O2; phenolic coumpounds; ultrasonic-assisted extraction

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Article Info

Section: Research Article

Language : EN

In Volume 14, No. 4, OKTOBER 2013

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