Oil Spill Biodegradation by Bacteria Isolated from Jakarta Bay Marine Sediments

A laboratory study was conducted with the aim to isolate and identify bacteria from sea sediment and test their biodegradation ability in two place where contaminated with oil spill. Five sediment samples were dissolved by using sterile sea water, and then bacteria isolated with total plate count (TPC) method. Isolates bacteria was cultivated, and adapted using the nutrient conditioned sea water medium. Biodegradation process was done by mixing the bacteria with crude oil and shaken for few days. The number of bacteria isolated varied from 2 x 102 CFU ml-1 to 6 x 106 CFU ml-1 and apparently increased after cultivation and adaptation with oily media. Bacteria identified during this study were Fundibacter sp., Alcanivorax sp., and Marinobacter sp.. The result of biodegradation process was statistically analyzed and obtained that the bacteria are effective in degrading oil in seven days with constant of biodegradation rate was 0.1766. GC-MS analysis was conducted to prove the decomposition of carbon chain by bacteria and revealed oil degradation in carbon number 11 to 27. Based on all analysis, marine sediment bacteria can degrade the oil spill.
Keywords : Bacteria, Biodegradation, GC-MS, Marine Sediments, Oil spill.
Article Metrics:
Last update: 2021-02-25 00:44:41
Last update: 2021-02-25 00:44:42
Copy this form and after filling it, please send it to ijms@undip.ac.id:
COPYRIGHT TRANSFER STATEMENT
When this article is accepted for publication, its copyright is transferred to ILMU KELAUTAN Indonesian Journal of Marine Sciences, UNDIP. The copyright transfer covers the exclusive right to reproduce and distribute the article, including reprints, translations, photographic reproductions, microform, electronic form (offline, online) or any other reproductions of similar nature.
The author warrants that this article is original and that the author has full power to publish. The author signs for and accepts responsibility for releasing this material on behalf of any and all co-authors. In regard to all kind of plagiarism in this manuscript, if any, only the author(s) will take full responsibility. If the article is based on or part of student’s skripsi, thesis or dissertation, the student needs to sign as his/her agreement that his/her works is going to be published.
Title of article :...........................................................................................................................
Name of Author(s) :...........................................................................................................................
Author’s signature :...........................................................................................................................
Date :...........................................................................................................................