1Faculty of Fisheries and Marine Science, Universitas Halu Oleo, Indonesia
2Faculty of Medicine, Universitas Halu Oleo, Indonesia
3Faculty of Pharmacy, Universitas Halu Oleo, Indonesia
4 Research Centre for Chemistry, Indonesia Institute of Sciences, Indonesia
5 Institute of Systems Biology, Universiti Kebangsaan Malaysia, Malaysia
6 Faculty of Pharmacy, Universitas Hau Oleo, Indonesia
BibTex Citation Data :
@article{IK.IJMS43254, author = {Baru Sadarun and Nur Rahmatika and Agung Yodha and Adryan Fristiohady and Andini Sundowo and Syarul Baharum and Idin Sahidin}, title = {Antioxidant and Cytotoxic Properties of Soft Coral Nepthea sp.}, journal = {ILMU KELAUTAN: Indonesian Journal of Marine Sciences}, volume = {27}, number = {1}, year = {2022}, keywords = {Nepthea sp.; non-polar fraction; antioxidant; cytotoxic; South East Sulawesi}, abstract = { Soft coral Nepthea sp. grows in the seas of South-East Sulawesi, Indonesia. However, information on the chemical and pharmaceutical aspects of this genus is still limited. Therefore, this research aims to explore the chemical contents and biological activities of Nepthea sp. The sample was collected from the waters of Saponda Island by SCUBA diving. It was extracted by ethyl acetate and fractionated using vacuum liquid chromatography. The chemical content was analyzed by phytochemical screening, LC-MS/MS analysis, Total Phenolics Content and Total Flavonoids Contents. Antioxidant potency was evaluated by DPPH ( 2,2-diphenyl-1-picrylhydrazyl) radicals and ABTS (2,2’-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid). Cytotoxicity property was analyzed by MTT (3-(4,5- Dimethy lthiazol- 2-yl)-2,5-d iphenylt etrazolium bromide) assays. The result showed that the fractionation of Nepthea ethylacetate extracts produced six fractions (A-F). Fractions A and B contain non-polar compounds. Based on LC-MS/MS data, the non-polar compounds in Fraction A and B include achillin, atractylenolide II, buthyl isobuthyl phthalate, rengyolester, 2a-acetoxycostic acid, ocotillol acetate, petasitolone and some unidentified compounds that are C 33 H 58 O 4 , C 15 H 21 NO, C 21 H 33 NO, and C 16 H 20 O 4 . In general, the antioxidant and cytotoxic properties of all samples are in the weak category, however, when examined for each sample, the antioxidant properties of fraction B is slightly better than fraction A based on the IC 50 value of DPPH and ABTS. Cytotoxicity of Fraction A is better than Fraction B against Breast Cancer cell lines MCF-7. The non-polar fraction of Nepthea sp. can be developed as raw material for the discovery of new compounds, antioxidant and anticancer agents, especially breast cancer. }, issn = {2406-7598}, pages = {29--36} doi = {10.14710/ik.ijms.27.1.29-36}, url = {https://ejournal.undip.ac.id/index.php/ijms/article/view/43254} }
Refworks Citation Data :
Soft coral Nepthea sp. grows in the seas of South-East Sulawesi, Indonesia. However, information on the chemical and pharmaceutical aspects of this genus is still limited. Therefore, this research aims to explore the chemical contents and biological activities of Nepthea sp. The sample was collected from the waters of Saponda Island by SCUBA diving. It was extracted by ethyl acetate and fractionated using vacuum liquid chromatography. The chemical content was analyzed by phytochemical screening, LC-MS/MS analysis, Total Phenolics Content and Total Flavonoids Contents. Antioxidant potency was evaluated by DPPH (2,2-diphenyl-1-picrylhydrazyl) radicals and ABTS (2,2’-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid). Cytotoxicity property was analyzed by MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assays. The result showed that the fractionation of Nepthea ethylacetate extracts produced six fractions (A-F). Fractions A and B contain non-polar compounds. Based on LC-MS/MS data, the non-polar compounds in Fraction A and B include achillin, atractylenolide II, buthyl isobuthyl phthalate, rengyolester, 2a-acetoxycostic acid, ocotillol acetate, petasitolone and some unidentified compounds that are C33H58O4, C15H21NO, C21H33NO, and C16H20O4. In general, the antioxidant and cytotoxic properties of all samples are in the weak category, however, when examined for each sample, the antioxidant properties of fraction B is slightly better than fraction A based on the IC50 value of DPPH and ABTS. Cytotoxicity of Fraction A is better than Fraction B against Breast Cancer cell lines MCF-7. The non-polar fraction of Nepthea sp. can be developed as raw material for the discovery of new compounds, antioxidant and anticancer agents, especially breast cancer.
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Antioxidant, Toxicity and Secondary Metabolites Contents of Ethylacetate Fraction from Soft Coral Lobophytum Sp. Growing in South East Sulawesi
In vivo Anti-Inflammatory and Immunomodulatory Activity of Soft Coral Nephthea sp. from Southeast Sulawesi
Last update: 2024-04-18 04:23:07
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