DOI: https://doi.org/10.14710/jil.18.1.58-66

Karakter Rhizobakteri Pelarut Fosfat Potensial dari Rhizosfer Tumbuhan Mangrove Teluk Awur Kabupaten Jepara secara Mikrobiologi

1Universitas Jenderal Soedirman, Indonesia

2Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro, Semarang, Indonesia

Received: 10 Dec 2019; Published: 29 Apr 2020.
Editor(s): Sudarno Utomo

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Abstract

Ekosistem mangrove Teluk Awur yang terletak di Kabupaten Jepara, Provinsi Jawa Tengah, merupakan  salah satu ekosistem mangrove yang mengalami kerusakan cukup parah karena perluasan lahan budidaya ikan (tambak), sehingga mendorong terjadinya erosi pantai. Peremajaan kembali dan konservasi ekosistem mangrove dapat dilakukan dengan menggunakan bakteri tanah yang mampu mendukung pertumbuhan tanaman atau yang biasa disebut Plant Growth Promoting Rhizobacteria (PGPR). Salah satu mekanisme pendukung pertumbuhan tanaman oleh kelompok PGPR adalah dengan aktivitas pelarutan fosfat karena fosfat dalam tanah berada dalam bentuk yang sulit diserap oleh tanaman. Penelitian ini bertujuan untuk memperoleh isolat Rhizobakteri pelarut fosfat yang unggul dalam melarutkan fosfat secara in-vitro dan mengetahui karakter isolat yang diperoleh. Isolasi dan penapisan rhizobakteri pelarut fosfat dilakukan menggunakan medium Pikovskaya agar. Karakterisasi isolat potensial dilakukan secara mikrobiologi dan atau uji aktivitas biokimia. Hasil penelitian menunjukkan bahwa rhizobakteri pelarut fosfat potensial yang berhasil diisolasi, secara mikrobiologi teridentifikasi ke dalam genus Enterobacter.

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Keywords: karakterisasi; rhizobakteri; fosfat; Teluk Awur; enterobacter
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  1. Ahemad, M & Khan, M.S., (2010), Plant Growth Promoting Activities of Phosphate Solubilizing Enterobacter asburiae as Influenced by Fungicides, Eur. Asian Journal of Biosciences, 4, 88-95
  2. Banerjee, S., Palit, R., Sengupta, C & Standing, D., (2010), Stress Induced Phosphate Solubilization by Arthrobacter sp. and Bacillus sp. Isolated from Tomato Rhizosphere, Australian Journal of Crop Science, 4(6), 378-383
  3. Bariusso, J., Solano, B.R., Lucas, J.A., Lobo, A.P., Garcia-Villaraco, A & Manero, F.J.G., (2008), Plant-Bacteria Interactions, Strategies and Techniques to Promote Plant Growth : Ecology, Genetic Diversity and Screening Strategies of Plant Growth Promoting Rhizobacteria (PGPR), Wiley-VCH Verlag GmbH & Co. KgaA, Weinhem
  4. Berg, J.M., Tymoczko, J.L., Stryer, L, (2002), Biochemistry 5th Edition, W H Freeman and Company, New York
  5. Bharatkumar, S., Rameshkumar, N., Paul, D., Prabavaty, V.R & Sudha Nair., (2008), Characterization of the Predominant Bacterial Population of Different Mangrove Rhizosphere Soil Using 16S rRNA Gene-Based Single-Strand Conformation Polymorphism (SSCP), World J Microbiol Biotechnol, 24, 387-394
  6. Calvo, P., Ormeño-Orrillo, E., Martínez-Romero, E. & Zúñiga, D., (2010), Characterizarion of Bacillus Isolates of Potato Rhizosphere from Andean Soils of Peru and Their Potential PGPR Characteristics, Brazilian Journal of Microbiology, 41, 899-906
  7. Cappucino, J.G. & Sherman, N., (1987), Microbiology: A Laboratory Manual, The Benjamin/Cummings Publishing Company Inc., California USA
  8. Cappucino, J.G. & Sherman, N., (2014), Microbiology: A Laboratory Manual, Pearson Education, United States of America
  9. Carmo, F.L., Santos, H.F., Martins, E.F., Elsas, J.D., Rosado, A.S. & Peixoto, R.S., (2011), Bacterial Structure and Characterization of Plant Growth and Oil Degrading Bacteria from the Rhizosphere of Mangrove Plants, The Journal of Microbiology, 49(4), 535-543
  10. Channarayappa, Biradar, D.P., (2019), Soil Basics, Management, and Rhizosphere Engineering for Sustainable Agriculture. CRC Press, Florida, USA
  11. Collins, M.E., (2003), Soil Section : Key to Soil Orders in Florida. Florida Envirothon Study Sections University of Florida, Florida, United States America
  12. Dastager, S.G., Deepa, C.K., Puneet, S.C., Nautiyal, C.S & Pandey, A., (2009), Isolation and Characterization of Plant Growth-Promoting Strain Pantoea NII-186. from Western Ghat Forest Soil, India, Journal Compilation 2009 The Society for Applied Microbiology, Letters in Applied Microbiology, 49, 20-25
  13. Dewi, I.R., (2007), Makalah : Rhizobacteria Pendukung Pertumbuhan Tanaman Plant Growth Promotor Rhizobacteria, Universitas Padjdjaran, Jatinangor
  14. Fachrul, M.F., (2007), Metode Sampling Bioekologi, Bumi Aksara, Jakarta
  15. Fardiaz, S., (1989), Mikrobiologi Pangan, Institut Pertanian Bogor, Bogor, Indonesia
  16. Ginting, R.C.B., Saraswati, R & Husen, E., (2005), 7. Mikroorganisme Pelarut Fosfat : Pupuk Organik & Pupuk Hayati, dalam Pupuk Hayati dan Pupuk Organik, ed Suriadikarta & Simanungkalit, hal. 141-158
  17. Girish, K., Shrikant, B., Sunil, M & Manish, D., (2010), Exploring the Potential of Pseudomonas Species as a Phosphate Solubilizer, Plant Growth Promoter, Biocontrol Agent and Pesticide Degrader. Asian J. Exp. Biol. Sci., 40-44
  18. Hariyono, (2007), Status Lingkungan Hidup Daerah Kabupaten Jepara, Dinas Lingkungan Hidup Pertambangan dan Energi. Jepara
  19. Hodges, S.C., (tanpa tahun), Soil Science Extension North Carolina University : Soil Fertility Basics, NC Certified Crop Advisor Training, North Carolina University, North Carolina
  20. Jha, B.K., Pragash, M.G., Cletus, J., Raman, G & Sakthivel, N., (2008), Simultaneous Phosphate Solubilization Potential and Antifungal Activity of New Fluorescent Pseudomonad Strains, Pseudomonas aeruginosa, P. plecoglossicida and P. Mosselii, World J Microbiol Biotechnol, 25, 573-581
  21. Kirk, J.L., Beaudette, L.A., Hart, M., Moutoglis, P, Klironomos, J.N., Lee, H & Trevors, J.T., (2004), Review : Methods of Studying Soil Microbial Diversity, Journal of Microbiological Methods, 58, 169– 188
  22. Kothamasi, D., Kothamasi, S., Bhattacharyya, A., Kuhad, R.C. & Babu, C.R., (2006), Arbuscular Mycorrhizae and Phosphate Solubilising Bacteria of the Rhizosphere of the Mangrove Ecosystem of Great Nicobar Island, India, Biol Fertil Soils, 42, 358–361
  23. Krull, E.S., Skjemstad, J.O & Baldock, J.A., (2005), GRDC Project No. CSO 00029 Residue Management, Soil Organic Carbon and Crop Performance: Functions of Soil Organic Matter and the Effect on Soil Properties, CSIRO Land & Water PMB 2 Glen Osmond S.A 5064
  24. Lee, K.D., Bai, Y., Smith, D., Han, H.S & Supanjani., (2005), Isolation of Plant-Growth-Promoting Endophytic Bacteria from Bean Nodules, Research Journal of Agriculture and Biological Sciences, 1(3), 232-236
  25. Marquis, R.E., (1968), Salt-Induced Contraction of Bacterial Cell Walls, Journal of Bacteriology, 95 (3), 775-781
  26. Mehta, S & Nautiyal, C.S., (2001), An Efficient Method for Qualitative Screening of Phosphate-Solubilizing Bacteria, Current Microbiology, 43, 51-56
  27. Mishra, R.R.,Dangar, T.K., Rath, B & Thatoi H, N., (2009), Characterization and Evaluation of Stress and Heavy Metal Tolerence of Some Predominant Gram Negative Halotolerant Bacteria From Mangrove Gills of Bhitarkanika, Orissa, India, African Journal of Biotechnology, 8(10), 2224-2231
  28. Nisa’, N.A., (2018), Isolasi dan Identifikasi Bakteri Pelarut Fosfat dengan Sekuens 16S rRNA Asal Tanah Pertanian Organik Desa Sumberejo Batu. Skripsi. Universitas Islam Negeri Maulana Malik Ibrahim, Malang
  29. Pangastuti, A., (2006), Review: Definisi Spesies Prokariota Berdasarkan Urutan Basa Gen Penyandi 16S rRNA dan Gen Penyandi Protein, Biodiversitas, 7 (3), 292- 296
  30. Pritchard, D.L., (2005), Muresk Institute : Phosphorus Bioavailability from Land-Applied Biosolids in South-Western Australia, Thesis, Curth University of Technology, Australia
  31. Ramesh, V., Wani, S.P., Rego, T.J., Sharma, K.L., Bhattacharyya, T., Sahrawat, K.L., Padmaja, K.V., Gangadhar Rao, D., Venkateswarlu, B., Vanaja, M., Manna, M.C., Sanvas, K & Maruthi, V., (2007), Chemical Characterization of Selected Benchmark Spots for C Sequestration in the Semi-Arid Tropics, India. International Crops Research Institute for the Semi-Arid Tropics. India
  32. Robinson, P.K., 2015. Enzymes: Principles and Biotechnological Applications. Essays Biochem, 59, 1-41
  33. Rodríguez, H., & Fraga, R., (1999), Phosphate Solubilizing Bacteria and Their Role in Plant Growth Promotion. Biotechnology Advances of Cuban Research Institute, 17, 319–339
  34. Sahoo, K & Dahl, N.K., (2009), Potential Microbial Diversity in Mangrove Ecosystems: A Review, Indian Journal of Marine Sciences, 38(2), 249-256
  35. Sudha Nair, Sasirekha, N., Appunu, C., Bharathkumar, S., Loganathan, P., Rameshkumar, N., Sridhar, R., Subathra, G & Prabhavathy, V.R., (2008), Microbial Diversity in Mangrove Ecosystem. M.S. Swaminathan Research Foundation 3rd Cross Road, Taramani Institutional Area, Chennai-600113. National Institute of Agricultural
  36. Sciences and Technology, Suwon, South Korea & Medical Entomology, Dept. of Entomology, N. C. State University, Raleigh
  37. Suwanto, A., (1994), Ulas Balik: Evolusi Mikrobe dan Kaitannya dengan Sistematik Molekuler, Hayati, 1 (2), 26-31
  38. Thuller, D.S., Floh, E.I.S., Handro, W & Barbosa, H.R., (2003), Plant Growth Regulator and Amino Acids Released by Azospirillum sp. in Chemically Defined Media, Letters in Applied Microbiology, 37, 174-178
  39. Waluyo, L., (2008), Teknik Metode Dasar Mikrobiologi. Universitas Muhamadiyah Malang Press, Malang
  40. Zhao, X. & Drlica, K., (2014), Reactive Oxygen Species and The Bacterial Response to Lethal Stress, Curr Opin Microbiol, 21, 1-6

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