Exploring effects of betaine-homocysteine methyltransferase (BHMT) gene polymorphisms on fatty acid traits and cholesterol in sheep

J. P. Munyaneza -  Graduate School of IPB University, IPB University Jl.Agatis, Dramaga Campus, Bogor 16680, Indonesia
*A. Gunawan -  Department of Animal Production and Technology, Faculty of Animal Science, IPB University Jl.Agatis, Dramaga Campus, Bogor 16680, Indonesia
R. R. Noor -  Department of Animal Production and Technology, Faculty of Animal Science, IPB University Jl.Agatis, Dramaga Campus, Bogor 16680, Indonesia
Received: 13 May 2019; Accepted: 6 Aug 2019; Published: 24 Sep 2019.
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Abstract

This study was aimed to explore the effects of Betaine-Homocysteine Methyltransferase (BHMT) gene polymorphisms on fatty acid traits and cholesterol in lambs. This study used a total of 147 blood samples for genotyping including 19 Javanese Fat-Tailed (JFT), 16 Javanese Thin-Tailed (JTT), 41 Composite Garut (CG), 35 Compass Agrinak (CA) and 36 Barbados Black Belly Cross (BC). A total of 61 rams as representative from five breed of sheep were selected for association study. Identification of BHMT single nucleotide polymorphisms was analyzed by PCR-RFLP method. Association of BHMT genotypes with fatty acid traits and cholesterol was performed by T-TEST. BHMT genotyping resulted into three genotypes (CC, CT and TT). Gene frequency of BHMT (g. 9947372 C>T) was in Hardy-Weinberg Equilibrium, excluding Javanese Fat-Tailed sheep. Association of BHMT genotypes with fatty acid traits resulted into a significant association (P<0.05) with C14:1, C17:1 and C18:0 fatty acids but not with cholesterol in sheep. SNP g. 9947372 (C>T) of BHMT gene might be a useful marker for selecting and producing sheep meat with desirable fatty acids.

 

Keywords
BHMT; Fatty acid; Sheep; SNP

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