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Uji Aktivitas Isolat L-Asparaginase Dari Rimpang Temulawak (Curcuma xanthorrhiza Roxb) Terhadap Sel Hela

1Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University, Indonesia

2Otolaryngology Department, Faculty of Medicine, Diponegoro University, Indonesia

Published: 1 Aug 2010.
Open Access Copyright 2010 Jurnal Kimia Sains dan Aplikasi under http://creativecommons.org/licenses/by-sa/4.0/.

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Abstract
L-asparaginase adalah enzim hidrolase yang mengkatalisis hidrolisis asparagin menjadi asam aspartat dan amonia. L-asparaginase diketahui membantu pengobatan kanker dengan menghambat sintetis protein sel kanker tanpa merusak sel normal. Metode yang dilakukan adalah isolasi enzim meliputi ekstraksi, fraksinasi dengan ammonium sulfat; karakterisasi dengan uji aktivitas spesifik dan uji sitotoksisitas pada sel HeLa. Aktivitas spesifik tertinggi isolat L-asparaginase fraksi amonium sulfat terdapat pada fraksi keempat sebesar 22,639 unit/mg. Selanjutnya fraksi keempat diujikan pada sel HeLa dalam uji sitotoksisitas. Berdasarkan hasil uji sitotoksisitas, isolat ini memiliki aktivitas penghambatan terhadap pertumbuhan sel HeLa dengan nilai LC50 sebesar 309,74 ppm
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Keywords: Asparaginase; temulawak; isolasi; HeLa

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