Uji Aktivitas Isolat L-Asparaginase Dari Rimpang Temulawak (Curcuma xanthorrhiza Roxb) Terhadap Sel Hela

Arthuro Romualdo  -  Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University, Indonesia
*Wuryanti Wuryanti  -  Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University, Indonesia
Suprihati Suprihati  -  Otolaryngology Department, Faculty of Medicine, Diponegoro University, Indonesia
Published: 1 Aug 2010.
Open Access Copyright 2010 Jurnal Kimia Sains dan Aplikasi
License URL: http://creativecommons.org/licenses/by-sa/4.0/

Citation Format:
Abstract
L-asparaginase adalah enzim hidrolase yang mengkatalisis hidrolisis asparagin menjadi asam aspartat dan amonia. L-asparaginase diketahui membantu pengobatan kanker dengan menghambat sintetis protein sel kanker tanpa merusak sel normal. Metode yang dilakukan adalah isolasi enzim meliputi ekstraksi, fraksinasi dengan ammonium sulfat; karakterisasi dengan uji aktivitas spesifik dan uji sitotoksisitas pada sel HeLa. Aktivitas spesifik tertinggi isolat L-asparaginase fraksi amonium sulfat terdapat pada fraksi keempat sebesar 22,639 unit/mg. Selanjutnya fraksi keempat diujikan pada sel HeLa dalam uji sitotoksisitas. Berdasarkan hasil uji sitotoksisitas, isolat ini memiliki aktivitas penghambatan terhadap pertumbuhan sel HeLa dengan nilai LC50 sebesar 309,74 ppm
Keywords: Asparaginase; temulawak; isolasi; HeLa

Article Metrics:

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Last update: 2021-05-17 07:51:24

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Last update: 2021-05-17 07:51:24

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