Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University, Indonesia
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@article{JKSA16717, author = {Sri Harjanto}, title = {Perbandingan Pembacaan Absorbansi Menggunakan Spectronic 20 D+ dan Spectrophotometer UV-Vis T 60U Dalam Penentuan Kadar Protein dengan Larutan Standar BSA}, journal = {Jurnal Kimia Sains dan Aplikasi}, volume = {20}, number = {3}, year = {2017}, keywords = {absorbansi; spectronic 20 D+; Spectrophotometer UV-Vis T 60U}, abstract = {Telah dilakukan uji perbandingan pada pembacaan Absorbansi dari penentuan kadar Protein menggunakan Spectronic 20 D+ dan spectrophotometer uv-vis T 60U pada panjang gelombang 600 nm. Penentuan kadar protein menurut metode lowry dilakukan dengan cara membuat larutan standar dengan berbagai tingkat konsentrasi, kemudian dari masing-masing konsentrasi tersebut diambil 1 mL dan ditambahkan larutan lowry B, kemudian dibiarkan selama 10 menit dan kemudian ditambahkan larutan lowry A untuk dibiarkan selama 20 menit lalu diukur absorbansinya pada panjang gelombang 600 nm. Hasil penelitian pengukuran absorbansi menggunakan spectronic 20D+ menunjukkan nilai R2 = 0,9927 sedangkan untuk Spectrophotometer uv-vis T 60U menunjukan nilai R2 = 0,9971. Hal ini dapat disimpulkan bahwa pengukuran absorbansi untuk kedua instrumen tidak berbeda secara signifikan.}, issn = {2597-9914}, pages = {114--116} doi = {10.14710/jksa.20.3.114-116}, url = {https://ejournal.undip.ac.id/index.php/ksa/article/view/16717} }
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