DOI: https://doi.org/10.14710/jksa.24.3.70-76

Antimicrobial Activities of Synthesized Silver Nanoparticles using Ethanol and Water Extract of Mirabilis Jalapa

1Department of Pharmacy, STIKes Prima Indonesia, Bekasi, Indonesia

2Department of Pharmacy, Binawan University, Indonesia

3Department of Medical Laboratory Technologist, STIKes Prima Indonesia, Indonesia

Received: 2 Feb 2021; Revised: 6 Mar 2021; Accepted: 12 Mar 2021; Published: 31 Mar 2021.
Open Access Copyright 2021 Jurnal Kimia Sains dan Aplikasi under http://creativecommons.org/licenses/by-sa/4.0.

Citation Format:
Cover Image
Abstract
The focus of this study was to compare the antimicrobial activity of silver nanoparticles (AgNPs) synthesized using ethanol extracts (AgNPE) and water extracts (AgNPA) from four o’clock flowers (Mirabilis jalapa) against Staphylococcus aureus. AgNPs were characterized by UV-Vis diffraction, FTIR, SEM, and X-rays. UV-Vis analysis showed that AgNPA has an SPR band of about 460 nm and 530 nm for AgNPE, which proves the characteristics of the absorbance area of AgNPs. SEM images of AgNPE and AgNPA show a cuboid shape with a mean diameter of 80 and 30 nm, respectively and well dispersed. The response to the presence of polysaccharide biomolecules involved in forming AgNPs was analyzed using a Fourier transform infrared spectrometer. The result was that AgNPA and AgNPE have different reducing agents. The plant extracts, AgNPE and AgNPA, were studied for their antimicrobial activity against Staphylococcus aureus. The result was that both AgNPA and AgNPE showed good activity and showed that AgNPA with less inhibition was more effective than AgNPE.
Fulltext View|Download Email colleagues
Keywords: antimicrobial activity; silver nanoparticles; Mirabilis jalapa; Staphylococcus aureus

Article Metrics:

Article Info
Section: Research Articles
Language : EN
Most cited articles
Pemanfaatan Karbon Aktif Serbuk Gergaji Kayu Jati untuk Menurunkan Chemical Oxygen Demand (COD) Limbah Cair Industri Tekstil Removal of natural organic matter for wetland saline water desalination by coagulation-pervaporation Pengaruh Waktu pada Pembentukan Kalsium Fosfat dengan Sistem Membran Selulosa Bakterial Pengaruh Keasaman Medium dan Imobilisasi Gugus Organik pada Karakter Silika Gel dari Abu Sekam Padi Analisis Pangan: Penentuan Angka Peroksida dan Asam Lemak Bebas pada Minyak Kedelai dengan Variasi Menggoreng More cited articles
  1. Chella Purushothaman Devatha, Arun K. Thalla, Chapter 7 - Green Synthesis of Nanomaterials, in: S. Mohan Bhagyaraj, O.S. Oluwafemi, N. Kalarikkal, S. Thomas (Eds.) Synthesis of Inorganic Nanomaterials, Woodhead Publishing, 2018, https://doi.org/10.1016/B978-0-08-101975-7.00007-5
  2. Ullah Naveed, Ahmad Habib, Asif Afzal Haq, Abdul Qadar Khan, Anwar Rabia, Antibacterial activities of genetic variants of Mirabilis jalapa, Pharmacognosy Journal, 2, 7, (2010), 181-184 https://doi.org/10.1016/S0975-3575(10)80089-6
  3. Chifundera Kusamba, Kizungu Byamana, Wa Mpoyi Mbuyi, Antibacterial activity of Mirabilis jalapa seed powder, Journal of Ethnopharmacology, 35, 2, (1991), 197-199 https://doi.org/10.1016/0378-8741(91)90073-M
  4. Jyotchna Gogoi, Khonamai Sewa Nakhuru, Rudragoud S. Policegoudra, Pronobesh Chattopadhyay, Ashok Kumar Rai, Vijay Veer, Isolation and characterization of bioactive components from Mirabilis jalapa L. radix, Journal of Traditional and Complementary Medicine, 6, 1, (2016), 41-47 https://doi.org/10.1016/j.jtcme.2014.11.028
  5. Kazuko Aoki, Alma Rosa Cortés, María del Carmen Ramírez, Martín Gómez-Hernández, Francisco J. López-Muñoz, Pharmacological study of antispasmodic activity of Mirabilis jalapa Linn flowers, Journal of Ethnopharmacology, 116, 1, (2008), 96-101 https://doi.org/10.1016/j.jep.2007.11.004
  6. Sumbal, Asifa Nadeem, Sania Naz, Joham Sarfraz Ali, Abdul Mannan, Muhammad Zia, Synthesis, characterization and biological activities of monometallic and bimetallic nanoparticles using Mirabilis jalapa leaf extract, Biotechnology Reports, 22, (2019), e00338 https://doi.org/10.1016/j.btre.2019.e00338
  7. Shao Feng Chen, Jian Ping Li, Kun Qian, Wei Ping Xu, Yang Lu, Wei Xin Huang, Shu Hong Yu, Large scale photochemical synthesis of M@TiO2 nanocomposites (M = Ag, Pd, Au, Pt) and their optical properties, CO oxidation performance, and antibacterial effect, Nano Research, 3, 4, (2010), 244-255 https://doi.org/10.1007/s12274-010-1027-z
  8. Huma Yousaf, Ansar Mehmood, Khawaja Shafique Ahmad, Muhammad Raffi, Green synthesis of silver nanoparticles and their applications as an alternative antibacterial and antioxidant agents, Materials Science and Engineering: C, 112, (2020), 110901 https://doi.org/10.1016/j.msec.2020.110901
  9. Mitchell L. Cohen, Staphylococcus aureus: Biology, mechanisms of virulence, epidemiology, The Journal of Pediatrics, 108, 5, Part 2, (1986), 796-799 https://doi.org/10.1016/S0022-3476(86)80747-8
  10. Adewumi Oluwasogo Dada, Folahan Amoo Adekola, Fehintoluwa Elizabeth Dada, Adunola Tabitha Adelani-Akande, Micheal Oluwasesan Bello, Chidiogo Rita Okonkwo, Adejumoke Abosede Inyinbor, Abimbola Peter Oluyori, Adeniyi Olayanju, Kolawole Oluseyi Ajanaku, Charles Oluwaseun Adetunji, Silver nanoparticle synthesis by Acalypha wilkesiana extract: phytochemical screening, characterization, influence of operational parameters, and preliminary antibacterial testing, Heliyon, 5, 10, (2019), e02517 https://doi.org/10.1016/j.heliyon.2019.e02517
  11. Is Fatimah, Nur Afisa Lintang Mutiara, Biosythesis of silver nanoparticles using Putri Malu (Mimosa pudica) leaves extract and microwave irradiation method, Molekul, 11, 2, (2016), 288-298 http://dx.doi.org/10.20884/1.jm.2016.11.2.221
  12. M. Gopalakrishnan, A. Sheenu, Binny Varghese, J. Dharani, S. Saranya, A Green Approach for Synthesis and Characterization of Silver Nanoparticlesand Their Anti-Microbial Activity, Rasayan Journal of Chemistry, 12, 3, (2019), 1072-1076 https://doi.org/10.31788/RJC.2019.1235136
  13. Mahendran Vanaja, Gurusamy Annadurai, Coleus aromaticus leaf extract mediated synthesis of silver nanoparticles and its bactericidal activity, Applied Nanoscience, 3, 3, (2013), 217-223 https://doi.org/10.1007/s13204-012-0121-9
  14. Aftab Ahmad, Fatima Syed, Akram Shah, Zahid Khan, Kamran Tahir, Arif Ullah Khan, Qipeng Yuan, Silver and gold nanoparticles from Sargentodoxa cuneata: synthesis, characterization and antileishmanial activity, RSC Advances, 5, 90, (2015), 73793-73806 https://doi.org/10.1039/C5RA13206A
  15. Tentu Nageswara Rao, Riyazuddin, P. Babji, Naushad Ahmad, Rais Ahmad Khan, Iftekhar Hassan, Syed Ali Shahzad, Fohad Mabood Husain, Green synthesis and structural classification of Acacia nilotica mediated-silver doped titanium oxide (Ag/TiO2) spherical nanoparticles: Assessment of its antimicrobial and anticancer activity, Saudi Journal of Biological Sciences, 26, 7, (2019), 1385-1391 https://doi.org/10.1016/j.sjbs.2019.09.005
  16. Shakeel Ahmed, Mudasir Ahmad, Babu Lal Swami, Saiqa Ikram, A review on plants extract mediated synthesis of silver nanoparticles for antimicrobial applications: A green expertise, Journal of Advanced Research, 7, 1, (2016), 17-28 https://doi.org/10.1016/j.jare.2015.02.007
  17. Vudagandla Sreenivasulu, Nadavala Siva Kumar, M. Suguna, Mohammad Asif, Ebrahim Al-Ghurabi, Z. Huang, Z. Zhen, Biosynthesis of Silver Nanoparticles using Mimosa Pudica Plant root extract: Characterization, Antibacterial Activity and Electrochemical Detection of Dopamine, International Journal of Electrochemical Science, 11, (2016), 9959-9971 https://doi.org/10.20964/2016.12.69
  18. Shakeel Ahmed, Saifullah, Mudasir Ahmad, Babu Lal Swami, Saiqa Ikram, Green synthesis of silver nanoparticles using Azadirachta indica aqueous leaf extract, Journal of Radiation Research and Applied Sciences, 9, 1, (2016), 1-7 https://doi.org/10.1016/j.jrras.2015.06.006
  19. Sepideh Hamedi, Seyed Abbas Shojaosadati, Rapid and green synthesis of silver nanoparticles using Diospyros lotus extract: Evaluation of their biological and catalytic activities, Polyhedron, 171, (2019), 172-180 https://doi.org/10.1016/j.poly.2019.07.010
  20. Gianluca Calestani, Introduction to crystallography, in: P.W. Hawkes, P.G. Merli, G. Calestani, M. Vittori-Antisari (Eds.) Advances in Imaging and Electron Physics, Elsevier, 2002, https://doi.org/10.1016/S1076-5670(02)80060-X
  21. Mohsen Zargar, Azizah Abdul Hamid, Fatima Abu Bakar, Mariana Nor Shamsudin, Kamyar Shameli, Fatemeh Jahanshiri, Farah Farahani, Green Synthesis and Antibacterial Effect of Silver Nanoparticles Using Vitex Negundo L, Molecules, 16, 8, (2011), 6667-6676 https://doi.org/10.3390/molecules16086667
  22. Bulent Ulug, M. Haluk Turkdemir, Ahmet Cicek, Ahmet Mete, Role of irradiation in the green synthesis of silver nanoparticles mediated by fig (Ficus carica) leaf extract, Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 135, (2015), 153-161 https://doi.org/10.1016/j.saa.2014.06.142
  23. Daizy Philip, Biosynthesis of Au, Ag and Au–Ag nanoparticles using edible mushroom extract, Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 73, 2, (2009), 374-381 https://doi.org/10.1016/j.saa.2009.02.037
  24. Tracy L. Collins, Elizabeth A. Markus, Daniel J. Hassett, Jayne B. Robinson, The Effect of a Cationic Porphyrin on Pseudomonas aeruginosa Biofilms, Current Microbiology, 61, 5, (2010), 411-416 https://doi.org/10.1007/s00284-010-9629-y
  25. Palaniselvam Kuppusamy, Mashitah M. Yusoff, Gaanty Pragas Maniam, Natanamurugaraj Govindan, Biosynthesis of metallic nanoparticles using plant derivatives and their new avenues in pharmacological applications – An updated report, Saudi Pharmaceutical Journal, 24, 4, (2016), 473-484 https://doi.org/10.1016/j.jsps.2014.11.013
  26. Tahir Rasheed, Muhammad Bilal, Hafiz M. N. Iqbal, Chuanlong Li, Green biosynthesis of silver nanoparticles using leaves extract of Artemisia vulgaris and their potential biomedical applications, Colloids and Surfaces B: Biointerfaces, 158, (2017), 408-415 https://doi.org/10.1016/j.colsurfb.2017.07.020
  27. Libor Kvítek, Aleš Panáček, Jana Soukupová, Milan Kolář, Renata Večeřová, Robert Prucek, Mirka Holecová, Radek Zbořil, Effect of Surfactants and Polymers on Stability and Antibacterial Activity of Silver Nanoparticles (NPs), The Journal of Physical Chemistry C, 112, 15, (2008), 5825-5834 https://doi.org/10.1021/jp711616v
  28. Veronica Lazar, Quorum sensing in biofilms – How to destroy the bacterial citadels or their cohesion/power?, Anaerobe, 17, 6, (2011), 280-285 https://doi.org/10.1016/j.anaerobe.2011.03.023
  29. Saravanan Periasamy, Hwang-Soo Joo, Anthony C. Duong, Thanh-Huy L. Bach, Vee Y. Tan, Som S. Chatterjee, Gordon Y. C. Cheung, Michael Otto, How Staphylococcus aureus biofilms develop their characteristic structure, Proceedings of the National Academy of Sciences, 109, 4, (2012), 1281 https://doi.org/10.1073/pnas.1115006109
  30. Daeyeon Lee, Robert E. Cohen, Michael F. Rubner, Antibacterial Properties of Ag Nanoparticle Loaded Multilayers and Formation of Magnetically Directed Antibacterial Microparticles, Langmuir, 21, 21, (2005), 9651-9659 https://doi.org/10.1021/la0513306

Last update:

  1. Kinetics of Formation and Characterization of Green Silver Nanoparticles of Ficus variegata Leaf Extract

    Synodalia C. Wattimena, Violin Ririmasse, Amos Killay, Philipus J. Patty. Jurnal Kimia Sains dan Aplikasi, 25 (1), 2022. doi: 10.14710/jksa.25.1.34-40

  2. Easy and eco-friendly way for silver nanoparticles synthesis using Lotus corniculatus L: characterization and antibacterial activity

    Bessi Assia, Kahina Hamza, Boubkeur Boudine, Chaouki Boudaren. International Journal of Chemistry and Technology, 8 (2), 2024. doi: 10.32571/ijct.1507097

Last update: 2026-08-31 07:37:50

No citation recorded.