DOI: https://doi.org/10.14710/reaktor.17.3.111-117

FRACTIONATION OF PHENOLIC COMPOUNDS FROM KAFFIR LIME (Citrus hystrix) PEEL EXTRACT AND EVALUATION OF ANTIOXIDANT ACTIVITY

Yusak Adi Wijaya  -  Chemical Engineering Department, Widya Mandala Catholic University Surabaya, Indonesia
Daniel Widyadinata  -  Chemical Engineering Department, Widya Mandala Catholic University Surabaya, Indonesia
*Wenny Irawaty  -  Chemical Engineering Department, Widya Mandala Catholic University Surabaya, Indonesia
Aning Ayucitra  -  Chemical Engineering Department, Widya Mandala Catholic University Surabaya, Indonesia
Open Access Copyright (c) 2017 REAKTOR under http://creativecommons.org/licenses/by-nc-sa/4.0.

Citation Format:
Abstract
Side effects of drug-based treatment observed in patients during degenerative diseases treatments has directed towards to the identification of plants with antioxidant activity. Kaffir lime peel was selected in this study. This work was aimed to investigate the effect of solvent (hexane, ethyl acetate and n-butanol) polarity on the fractionation of ethanolic crude extract and assess its antioxidative property by DPPH radical scavenging assay. The results show semi polar solvent of ethyl acetate exhibited the best solvent to extract phenolic compounds from ethanolic kaffir lime peel with total phenolic content detected was 0.12 mg Gallic Acid Equivalent/mg. The employment of solvents possessing different polarity resulted several fractions, i.e. hexane, ethyl acetate, n-butanol and residue with different type of phenolic compounds in each fraction. Accordingly, each fraction exhibited different antioxidant activity against free radical compound of DPPH. The N-butanol fraction demonstrated the strongest activity which is shown by lowest IC50 value among the fractions tested. The fraction of n-butanol exhibited the IC50 value of 0.44 mg/mL which means that only 0.44 mg of the fraction/mL is required to inhibit the neutralization of DPPH by 50%. Phenolic identification by High Performance Liquid Chromatography indicates the different phenolic compounds in each fraction that contribute to antioxidative property to different extent. Further investigation to identify these phenolic compounds will lead to further development of kaffir lime as natural antioxidant to treat specific degenerative diseases.
Fulltext View|Download
Keywords: antioxidant; fractionation; kaffir lime (Citrus hystrix); peel

Article Metrics:

Article Info
Section: Research Article
Language : EN
Recent articles
Front Matter Reaktor Vol 17 No. 3 September 2017 Ekstraksi Asam Lemak Bebas dari Minyak Dedak Padi Menggunakan Etanol-Air dalam Tangki Pengaduk PENDUGAAN UMUR SIMPAN KERIPIK PISANG SALUT COKELAT “PURBARASA” KEMASAN POLIPROPILEN BERDASARKAN ANGKA TBA DENGAN METODE ASLT MODEL ARRHENIUS Pemetaan Pengaruh Proses Pengolahan pada Kualitas Biji Kakao Menggunakan Metode Metabolik Profiling dengan GC/MS Back Matter Reaktor No. 17 No. 3 September 2017 More recent articles
Most cited articles
PEMILIHAN ADSORBEN UNTUK PENJERAPAN KARBON MONOKSIDA MENGGUNAKAN MODEL ADSORPSI ISOTERMIS LANGMUIR Effect of Peanut Shell Torrefaction on Qualities of The Produced Bio-pellet Karakterisasi Pengemas Kertas Aktif dengan Penambahan Oleoresin dari Ampas Pengepresan Rimpang Temulawak (Curcuma xanthorrhiza Roxb) INFLUENCE OF INITIAL pH SOLUTION ON BIOFILM FORMATION AND CORROSION OF CARBON STEEL BY Serratia marcescens IN SITU TRANSESTERIFIKASI MINYAK BIJI MAHONI MENJADI METIL ESTER DENGAN CO-SOLVENT THF (TETRAHYDROFURAN) More cited articles
  1. Anagnostopoulou, M.A., Kefalas, P., Papageorgiou, V.P., Assimopoulou, A.N., and Boskou, D., (2006), Radical Scavenging Activity of Various Extracts and Fractions of Sweet Orange Peel (Citrus Sinensis), Food Chemistry, 94, pp. 19-25
  2. Baniasadi, H., Vlahakis, C., Hazebroek, J., Zhong, C., and Asiago, V., (2014), Effect of Environment and Genotype on Commercial Maize Hybrids Using Lc/Ms-Based Metabolomics, Journal of Agricultural and Food Chemistry, 62(6), pp. 1412-1422
  3. Barchan, A., Bakkali, M., Arakrak, A., Pagán, R., and Laglaoui, A., (2014), The Effects of Solvents Polarity on the Phenolic Contents and Antioxidant Activity of Three Mentha Species Extracts, International Journal of Current Microbiology and Applied Sciences, 3(11), pp. 399-412
  4. Chan, S.W., Lee, C.Y., Yap, C.F., Aida, W.M.W., and Ho, C.W., (2009), Optimisation of Extraction Conditions for Phenolic Compounds from Limau Purut (Citrus Hystrix) Peels, International Food Research Journal, 16, pp. 203-213
  5. Gadkari, P.V., and Balaraman, M., (2015), Catechins: Sources, Extraction and Encapsulation: A Review, Food and Bioproducts Processing, 93, pp. 122-138
  6. Garg, A., Garg, S., Zaneveld, L.J.D., and Singla, A.K., (2001), Chemistry and Pharmacology of the Citrus Bioflavonoid Hesperidin, Phytotherapy Research, 15(8), pp. 655-669
  7. Gebruers, K., Dornez, E., Bedo, Z., Rakszegi, M., Courtin, C.M., and Delcour, J.A., (2010), Variability in Xylanase and Xylanase Inhibition Activities in Different Cereals in the Healthgrain Diversity Screen and Contribution of Environment and Genotype to This Variability in Common Wheat, Journal of Agricultural and Food Chemistry, 58(17), pp. 9362-9371
  8. Gorinstein, S., Martı́n-Belloso, O., Lojek, A., Ĉı́ž, M., Soliva-Fortuny, R., Park, Y.-S., Caspi, A., Libman, I., and Trakhtenberg, S., (2002), Comparative Content of Some Phytochemicals in Spanish Apples, Peaches and Pears, Journal of the Science of Food and Agriculture, 82(10), pp. 1166-1170
  9. Irawaty, W., Soetaredjo, F.E., Ayucitra, A., Sianto, M.E., Jonathan, K., Hartono, C.D., Setyabudi, C., and Tanda, S., (2014), Antioxidant and Antidiabetic Activities of Ethanolic Citrus Hystrix Peel Extract: Optimization of Extraction Conditions, Australian Journal of Basic and Applied Sciences, 8(14), pp. 85-89
  10. Jamilah, B., Man, Y.B.C., and Ching, T.L., (2007), Antioxidant Activity of Citrus Hystrix Peel Extract in Rbd Palm Oleen During Frying of Fish Crackers, Journal of Food Lipids, 5(2), pp. 149-157
  11. Kanatt, S.R., Chander, R., and Sharma, A., (2007), Antioxidant Potential of Min (Mentha Spicata L.) in Radiation Processed Lamb Meat, Food Chemistry, 100, pp. 451-458

Last update:

  1. Underutilized citrus species: An insight of their nutraceutical potential and importance for the development of functional food

    Vikas Kumar, Ramandeep Kaur, Poonam Aggarwal, Gurvinder Singh. Scientia Horticulturae, 296 , 2022. doi: 10.1016/j.scienta.2022.110909

  2. The Use of Kaffir Lime Peel Filtrate (Citrus Hystrix) in Reducing The Number of Cutlery Germs

    Rusmiati Rusmiati, Deffany Novitasari Putri Suwanta, Putri Arida Ipmawati, Marlik Marlik. Jurnal Kesehatan Lingkungan Indonesia, 22 (1), 2023. doi: 10.14710/jkli.22.1.55-59

  3. Phytochemicals and In Vitro Bioactivities of Aqueous Ethanolic Extracts from Common Vegetables in Thai Food

    Uthaiwan Suttisansanee, Parunya Thiyajai, Parisut Chalermchaiwat, Khanitha Wongwathanarat, Kanchana Pruesapan, Somsri Charoenkiatkul, Piya Temviriyanukul. Plants, 10 (8), 2021. doi: 10.3390/plants10081563

  4. Antioxidant and anti-inflammatory activity through inhibition of NF-κB and sEH of some citrus peel and phytoconstituent characteristics

    Yesi Desmiaty, Ni Made Dwi Sandhiutami, Esti Mulatsari, Farah Alida Maziyah, Kartika Rahmadhani, Hanif Omar Zidane Algifari, Fernanda Alif Jantuna. Saudi Pharmaceutical Journal, 32 (2), 2024. doi: 10.1016/j.jsps.2024.101959

  5. Reduction of Graphene Oxide Using Citrus hystrix Peels Extract for Methylene Blue Adsorption

    Veronika Priliana, Clarissa Sucitro, Ronald Wijaya, Valentino Bervia Lunardi, Shella Permatasari Santoso, Maria Yuliana, Chintya Gunarto, Artik Elisa Angkawijaya, Wenny Irawaty. Sustainability, 14 (19), 2022. doi: 10.3390/su141912172

  6. Evaluation of Phytochemical Contents and In Vitro Antioxidant, Anti-Inflammatory, and Anticancer Activities of Black Rice Leaf (Oryza sativa L.) Extract and Its Fractions

    Chorpaka Thepthanee, Chan-Chiung Liu, Hsu-Sheng Yu, Ho-Shin Huang, Chia-Hung Yen, Yen-Hsien Li, Maw-Rong Lee, Ean-Tun Liaw. Foods, 10 (12), 2021. doi: 10.3390/foods10122987

  7. In vitro pharmacological screening of antioxidant, cytotoxic and enzyme inhibitory activities of Citrus aurantifolia Linn. Dried fruit extract

    Gökçe Şeker Karatoprak, Çiğdem Yücel Aşık, Ahmet Çakır, Esra Köngül Şafak. International Journal of Environmental Health Research, 31 (8), 2021. doi: 10.1080/09603123.2020.1714558

  8. Pharmacological and insecticidal attributes of common fruit peels: a review

    Shweta Gupta, Shivani Sharma, Neha Loach, Arun Pratap Sikarwar, Lalit Mohan. Environment, Development and Sustainability, 2024. doi: 10.1007/s10668-024-04998-4

  9. Bioactive compounds of Citrus hystrix peel ethanolic extract and their antioxidant potential under hydrogen peroxide-induced oxidative stress in Caco-2 cells

    Chrisyella Cendara Purba, Yunika Mayangsari, Widiastuti Setyaningsih, Worrapanit Chansuwan, Nualpun Sirinupong. Future Foods, 9 , 2024. doi: 10.1016/j.fufo.2024.100350

  10. Determination of bioactive compounds, antioxidant and anticancer activities of Tuckeroo (Cupaniopsis anacardioides) fruits

    Ngoc Minh Quynh Pham, Quan V. Vuong, Jennette A. Sakoff, Michael C. Bowyer, Van Anh Le, Christopher J. Scarlett. 3 Biotech, 12 (10), 2022. doi: 10.1007/s13205-022-03314-z

  11. The potency of Citrus hystrix DC in increasing fertility: The effect on ovarian follicle in Rattus norvegicus

    Eko Susetyarini, Sri Wahyuni, Yatimin, Ahmad Fauzi. PROCEEDINGS OF THE 3RD INTERNATIONAL SEMINAR ON METALLURGY AND MATERIALS (ISMM2019): Exploring New Innovation in Metallurgy and Materials, 2232 , 2020. doi: 10.1063/5.0002432

  12. Potential of the Ethyl Acetate Fraction of Padina boergesenii as a Natural UV Filter in Sunscreen Cream Formulation

    Soolmaz Soleimani, Morteza Yousefzadi, Sepideh Babaei Mahani Nezhad, Olga N. Pozharitskaya, Alexander N. Shikov. Life, 13 (1), 2023. doi: 10.3390/life13010239

  13. The fractions of phenolic and flavonoid compounds of the leaves of north Sulawesi’s bashful plant (mimosa pudica linn)

    D Rahardiyan, M Poluakan, E M Moko, J Ngangi. Journal of Physics: Conference Series, 1317 (1), 2019. doi: 10.1088/1742-6596/1317/1/012066

  14. Microencapsulation of Citrus Hystrix Essential Oil by Gelatin B/Chitosan Complex Coacervation Technique

    Siti Afiqah ‘Aisyah Murtadza, Nurul Asyikin Md Zaki, Junaidah Jai, Fazlena Hamzah, Nur Suhanawati Ashaari, Dewi Selvia Fardhyanti, Megawati Megawati, Nadya Alfa Cahaya Imani. Pertanika Journal of Science and Technology, 32 (S1), 2024. doi: 10.47836/pjst.32.2.07

  15. Design of kaffir lime peel extract tablets by direct compression: Effects of filler-binder on rheological properties

    Wenny Irawaty, Lannie Hadisoewignyo, Jenifer Wijaya, Shindy Saera Sababalat, Andy Sebastian Tanjaya. EXPLORING RESOURCES, PROCESS AND DESIGN FOR SUSTAINABLE URBAN DEVELOPMENT: Proceedings of the 5th International Conference on Engineering, Technology, and Industrial Application (ICETIA) 2018, 2114 , 2019. doi: 10.1063/1.5112390

  16. Charting the Sustainable Future of ASEAN in Science and Technology

    Rohayu Ramli, Nureen Shahirah Ahmad Zaghlul, Nor Atikah Husna Ahmad Nasir. 2020. doi: 10.1007/978-981-15-3434-8_11

Last update: 2024-11-13 15:32:44

No citation recorded.