BibTex Citation Data :
@article{Reaktor27531, author = {Diah Meilany and Dewinka Anugeraheni and Abdul Aziz and Made Tri Ari Kresnowati and Tjandra Setiadi}, title = {The Effects of Operational Conditions in Scaling Up of Xylanase Enzyme Production for Xylitol Production}, journal = {Reaktor}, volume = {20}, number = {1}, year = {2020}, keywords = {}, abstract = { The biological route to produce xylitol from Oil Palm Empty Fruit Bunches (EFBs) comprises of EFBs pretreatment, enzymatic hydrolysis, fermentation, and downstream separation of the produced xylitol. Due to the specificity in the hemicellulose composition of EFBs, a xylanase enzyme that has a high affinity to EFBs is required to hydrolyze the EFBs into xylose. In this research, the influences of aeration, humidity, and mixing in xylanase production were mapped. The xylanase production was performed by Aspergillus fumigatus ITBCCL170 in a solid-state fermentation using a tray fermenter with EFBs as the substrate. The optimal configuration was further scaled up into xylanase production using 1000 g of EFBs as the substrate. The results showed that the highest enzyme activity was 236.3 U/g EFB, obtained from the use of humid air airflow of 0.1 LPM, and mixing was performed once a day. The scaling up resulted in a lower xylanase activity and call for a better design of the fermenter. Keywords: aeration, humidity, mixing, OPEFBs, tray fermenter, xylanase, xylitol }, issn = {2407-5973}, pages = {32--37} doi = {10.14710/reaktor.20.1.32-37}, url = {https://ejournal.undip.ac.id/index.php/reaktor/article/view/27531} }
Refworks Citation Data :
The biological route to produce xylitol from Oil Palm Empty Fruit Bunches (EFBs) comprises of EFBs pretreatment, enzymatic hydrolysis, fermentation, and downstream separation of the produced xylitol. Due to the specificity in the hemicellulose composition of EFBs, a xylanase enzyme that has a high affinity to EFBs is required to hydrolyze the EFBs into xylose. In this research, the influences of aeration, humidity, and mixing in xylanase production were mapped. The xylanase production was performed by Aspergillus fumigatus ITBCCL170 in a solid-state fermentation using a tray fermenter with EFBs as the substrate. The optimal configuration was further scaled up into xylanase production using 1000 g of EFBs as the substrate. The results showed that the highest enzyme activity was 236.3 U/g EFB, obtained from the use of humid air airflow of 0.1 LPM, and mixing was performed once a day. The scaling up resulted in a lower xylanase activity and call for a better design of the fermenter.
Keywords: aeration, humidity, mixing, OPEFBs, tray fermenter, xylanase, xylitol
Article Metrics:
Last update:
Yellow laccase produced by Trametes versicolor K1 on tomato waste: A comparative study with the blue one produced on semi-synthetic medium
Evaluation of Heat Distribution And Aeration On Xylanase Production From Oil Palm Empty Fruit Bunches Using Tray Bioreactor
Purification and Characterization of Xylanase Produced by Aspergillus fumigatus Isolated from the Northern Border Region of Saudi Arabia
Last update: 2024-11-12 02:39:35
JURNAL REAKTOR (p-ISSN: 0852-0798; e-ISSN: 2407-5973)
Published by Departement of Chemical Engineering, Diponegoro University