BIODELIGNIFIKASI ENCENG GONDOK UNTUKMENINGKATKAN DIGESTIBILITAS PADA PROSESHIDROLISIS ENZIMATIK

Eka Sari; S Syamsiah; Hary Sulistyo; Muslikhin Hidayat

doi:10.14710/reaktor.17.1.54-60

DOI: https://doi.org/10.14710/reaktor.17.1.54-60

BIODELIGNIFIKASI ENCENG GONDOK UNTUKMENINGKATKAN DIGESTIBILITAS PADA PROSESHIDROLISIS ENZIMATIK

*Eka Sari - , Indonesia

S Syamsiah - Jurusan Teknik Kimia Fakultas Teknik, Universitas Gadjah Mada

Hary Sulistyo - Jurusan Teknik Kimia Fakultas Teknik, Universitas Gadjah Mada

Muslikhin Hidayat - Jurusan Teknik Kimia Fakultas Teknik, Universitas Gadjah Mada

Received: 5 Nov 2015; Published: 29 Mar 2017.

BibTex Citation Data :

@article{Reaktor9395,
author = {Eka Sari and S Syamsiah and Hary Sulistyo and Muslikhin Hidayat},
title = {BIODELIGNIFIKASI ENCENG GONDOK UNTUKMENINGKATKAN DIGESTIBILITAS PADA PROSESHIDROLISIS ENZIMATIK},
journal = {Reaktor},
volume = {17},
number = {1},
year = {2017},
keywords = {},
abstract = { A bstract Water hyacinth is one of lignocellulose that has potential to bioethanol feedstocks. This is due to the high content of cellulose and hemicellulose its rapid growth and the plant is abundant in nature. Bioconversion of water hyacinth begins with biodelignification process. This biodelignification aims to remove lignin and reduce crystallinity of cellulose and hemicellulose to increase its digestibility in enzymatic hydrolysis processes. The purpose of this study was to evaluate the effect of biodelignification of water hyacinth using fungi and the effect on the digestibility of water hyacinth in the enzymatic hydrolysis process. In this study, Biodelignification using Solid State Fermentation (SSF) method using Phanerochaete Chrysosporium (PC) . Hyacinth is inserted in a bioreactor and incubated for 28 days. The analysis of composition of water hyacinth is conducted every 4 days. After the biodelignification process followed by hydrolysis process using cellulase enzyme. Glucose generated from this hydrolysis process will be analyzed using the Somogyi Nelson method . The results show that biodelignification process can improve the digestibility of water hyacinths characterized by increased glucose yield. Without biodelignification process, glucose yield only reached 3.98%. After biodelignification prior to the hydrolysis process, the highest glucose yield was achieved at 67.66% for 16 days incubation. Keywords: biodeglinification ; glucose ; hydrolysis ; lignin ; cellulast Abstrak Enceng gondok adalah salah satu lignoselulosa yang berpotensi untuk dijadikan bahan baku bioetanol. Hal ini disebabkan oleh kandungan selulosa dan hemiselulosa yang tinggi dan pertumbuhannya yang cepat sehingga ketersediaannya di alam sangat melimpah. Biokonversi enceng gondok dimulai dengan proses biodelignifikasi. Biodelignifikasi ini bertujuan menghilangkan lignin dan mengurangi kristalinitas selulosa dan hemiselulosa untuk meningkatkan digestibilitasnya pada proses hidrolisis enzimatik. Tujuan dari penelitian ini adalah mengevaluasi pengaruh biodelignifikasi enceng gondok dengan jamur terhadap digestibilitas enceng gondok pada proses hidrolisis enzimatik. Biodelignifikasi pada penelitian ini menggunakan metode Solid State Fermentation (SSF) menggunakan jamur Phanerochaete Chrysosporium (PC). Enceng gondok dimasukan dalam sebuah bioreaktor dan diinkubasi selama 28 hari. Analisis perubahan komposisi enceng gondok dilakukan setiap 4 hari. Setelah proses biodelignifikasi dilanjutkan dengan proses hidrolisis menggunakan enzim selulase. Glukosa yang dihasilkan dari proses hidrolisis ini akan dianalisis menggunakan metode Somogyi Nelson. Hasil penelitian menunjukkan bahwa proses biodelignifikasi dapat meningkatkan digestibilitas enceng gondok yang ditandai dengan meningkatnya yield glukosa. Tanpa proses biodelignifikasi, yield glukosa hanya mencapai 3,98%. Setelah dilakukan biodelignifikasi sebelum proses hidrolisis maka yield glukosa tertinggi dicapai sebesar 67,66% selama 16 hari inkubasi. Kata kunci: biodeglinifikasi ; glukosa ; hidrolisis ; lignin ; selulase },
issn = {2407-5973}, pages = {54--60} doi = {10.14710/reaktor.17.1.54-60},
url = {https://ejournal.undip.ac.id/index.php/reaktor/article/view/9395}
}

Citation Format:

Abstract

Abstract

Water hyacinth is one of lignocellulose that has potential to bioethanol feedstocks. This is due to the high content of cellulose and hemicellulose its rapid growth and the plant is abundant in nature. Bioconversion of water hyacinth begins with biodelignification process. This biodelignification aims to remove lignin and reduce crystallinity of cellulose and hemicellulose to increase its digestibility in enzymatic hydrolysis processes. The purpose of this study was to evaluate the effect of biodelignification of water hyacinth using fungi and the effect on the digestibility of water hyacinth in the enzymatic hydrolysis process. In this study, Biodelignification using Solid State Fermentation (SSF) method using Phanerochaete Chrysosporium (PC). Hyacinth is inserted in a bioreactor and incubated for 28 days. The analysis of composition of water hyacinth is conducted every 4 days. After the biodelignification process followed by hydrolysis process using cellulase enzyme. Glucose generated from this hydrolysis process will be analyzed using the Somogyi Nelson method. The results show that biodelignification process can improve the digestibility of water hyacinths characterized by increased glucose yield. Without biodelignification process, glucose yield only reached 3.98%. After biodelignification prior to the hydrolysis process, the highest glucose yield was achieved at 67.66% for 16 days incubation.

Keywords: biodeglinification;glucose;hydrolysis;lignin; cellulast

Abstrak

Enceng gondok adalah salah satu lignoselulosa yang berpotensi untuk dijadikan bahan baku bioetanol. Hal ini disebabkan oleh kandungan selulosa dan hemiselulosa yang tinggi dan pertumbuhannya yang cepat sehingga ketersediaannya di alam sangat melimpah. Biokonversi enceng gondok dimulai dengan proses biodelignifikasi. Biodelignifikasi ini bertujuan menghilangkan lignin dan mengurangi kristalinitas selulosa dan hemiselulosa untuk meningkatkan digestibilitasnya pada proses hidrolisis enzimatik. Tujuan dari penelitian ini adalah mengevaluasi pengaruh biodelignifikasi enceng gondok dengan jamur terhadap digestibilitas enceng gondok pada proses hidrolisis enzimatik. Biodelignifikasi pada penelitian ini menggunakan metode Solid State Fermentation (SSF) menggunakan jamur Phanerochaete Chrysosporium (PC). Enceng gondok dimasukan dalam sebuah bioreaktor dan diinkubasi selama 28 hari. Analisis perubahan komposisi enceng gondok dilakukan setiap 4 hari. Setelah proses biodelignifikasi dilanjutkan dengan proses hidrolisis menggunakan enzim selulase. Glukosa yang dihasilkan dari proses hidrolisis ini akan dianalisis menggunakan metode Somogyi Nelson. Hasil penelitian menunjukkan bahwa proses biodelignifikasi dapat meningkatkan digestibilitas enceng gondok yang ditandai dengan meningkatnya yield glukosa. Tanpa proses biodelignifikasi, yield glukosa hanya mencapai 3,98%. Setelah dilakukan biodelignifikasi sebelum proses hidrolisis maka yield glukosa tertinggi dicapai sebesar 67,66% selama 16 hari inkubasi.

Kata kunci:biodeglinifikasi;glukosa;hidrolisis;lignin; selulase

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Section: Research Article

Language : EN

In Volume 17 No.1 Maret 2017

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