Produksi Dan Ekstraksi Inhibitor Alfa Glukosidase Dari Isolat Aktinomiset Jp-3

DOI: https://doi.org/10.14710/bioma.17.2.123-129

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Published: 12-01-2016
Section: Articles
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Alpha-glucosidase inhibitors are compounds that can prevent the digestion of complex carbohydrates into glucose, so potentially used as a diabetes drug. This study aims to examine the production and extraction of alpha-glucosidase inhibitor compound from Isolate Aktinomiset JP-3 from the sea. The supernatant obtained from the culture of the JP-3 isolate was extracted using various solvents to obtain the active compound. The solvents used were chloroform, methanol, and ethyl acetate. An assay of inhibitor activity of the α-glucosidase using p-nitrophenyl α-D-glucopyranoside substrate. The activity of the enzyme is measured based on the absorbance of p-nitrophenol produced from the breaking reaction of the substrate. The results showed that extraction of alpha-glucosidase inhibitor compound with ethyl acetate yielded extract with highest inhibitor activity.

 

Keywords: alpha-glucosidase inhibitors, actinomycetes, diabetes, extraction, fractionation