1Departemen Teknologi Hasil Perairan, Fakultas Perikanan Ilmu Kelautan, Institut Pertanian Bogor, , Indonesia
2Jl. Lingkar Akademik Kampus IPB Darmaga Bogor 16680, , Indonesia
3Telp.(0251)8622915, Fax. (0251) 8622916, nurhayati7870@yahoo.com, Indonesia
BibTex Citation Data :
@article{IK.IJMS1418, author = {Tati Nurhayati and Muhammad Fikri and Desniar Desniar}, title = {Aktivitas Inhibitor Protease dari Ekstrak Karang Lunak, Asal Perairan Pulau Panggang Kepulauan Seribu}, journal = {ILMU KELAUTAN: Indonesian Journal of Marine Sciences}, volume = {15}, number = {2}, year = {2012}, keywords = {}, abstract = { Beberapa komponen bioaktif dihasilkan oleh karang lunak, salah satunya inhibitor protease. Tujuan penelitian ini adalah mendapatkan karang lunak yang berpotensi sebagai penghambat aktivitas kerja enzim protease (inhibitor protease) pada beberapa bakteri patogen penghasil enzim protease serta mengetahui Minimum Inhibitory Concentration (MIC) dari ekstrak karang lunak tersebut. Hasil penelitian menunjukkan bahwa pelarut metanol lebih potensial untuk mengekstrak inhibitor protease dari karang lunak. Karang lunak jenis Sarcophyton sp. dan Sinularia sp. mampu menghambat 100% aktivitas protease bakteri Staphylococus aureus dengan MIC 0,04% lebih kecil dari pada MIC EDTA (0,16%), sedangkan Xenia sp. menghambat protease bakteri S. aureus dengan MIC 0,08%. Karang lunak Nephthea sp. menghambat protease bakteri Pseudomonas aeruginosa dengan MIC 0,28%. Kata kunci : inhibitor protease, karang lunak, MIC Several bioactive compounds were produced by soft corals, including protease inhibitor.The aim of this study was to obtain softcorals which potency as inhibitor toward protease enzyme activity on pathogenic bacterial that produced protease enzyme and to study Minimum Inhibitory Concentration (MIC) from the softcorals. This research shown that ethanol is more potential for extracting protease inhibitor from softcorals. Sarcophyton sp . and Sinularia sp . are capable of inhibiting protease enzyme activity against Staphylococus aureus as 100% by MIC 0.04%, while that EDTA had MIC toward the protease as 0.16%. Xenia sp . was capable of inhibiting protease from S. aureus by MIC 0.08%. In the otherhand Nephtea sp . inhibited protease from Pseudomonas aeruginosa by MIC 0.28%. Key words: protease inhibitor, soft coral, MIC. }, issn = {2406-7598}, pages = {59--65} doi = {10.14710/ik.ijms.15.2.59-65}, url = {https://ejournal.undip.ac.id/index.php/ijms/article/view/1418} }
Refworks Citation Data :
Beberapa komponen bioaktif dihasilkan oleh karang lunak, salah satunya inhibitor protease. Tujuan penelitian ini adalah mendapatkan karang lunak yang berpotensi sebagai penghambat aktivitas kerja enzim protease (inhibitor protease) pada beberapa bakteri patogen penghasil enzim protease serta mengetahui Minimum Inhibitory Concentration (MIC) dari ekstrak karang lunak tersebut. Hasil penelitian menunjukkan bahwa pelarut metanol lebih potensial untuk mengekstrak inhibitor protease dari karang lunak. Karang lunak jenis Sarcophyton sp. dan Sinularia sp. mampu menghambat 100% aktivitas protease bakteri Staphylococus aureus dengan MIC 0,04% lebih kecil dari pada MIC EDTA (0,16%), sedangkan Xenia sp. menghambat protease bakteri S. aureus dengan MIC 0,08%. Karang lunak Nephthea sp. menghambat protease bakteri Pseudomonas aeruginosa dengan MIC 0,28%. Kata kunci : inhibitor protease, karang lunak, MIC
Several bioactive compounds were produced by soft corals, including protease inhibitor.The aim of this study was to obtain softcorals which potency as inhibitor toward protease enzyme activity on pathogenic bacterial that produced protease enzyme and to study Minimum Inhibitory Concentration (MIC) from the softcorals. This research shown that ethanol is more potential for extracting protease inhibitor from softcorals. Sarcophyton sp. and Sinularia sp. are capable of inhibiting protease enzyme activity against Staphylococus aureus as 100% by MIC 0.04%, while that EDTA had MIC toward the protease as 0.16%. Xenia sp. was capable of inhibiting protease from S. aureus by MIC 0.08%. In the otherhand Nephtea sp. inhibited protease from Pseudomonas aeruginosa by MIC 0.28%.
Key words: protease inhibitor, soft coral, MIC.
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