1Magister of Marine Science Study Program, Faculty of Fisheries and Marine, Universitas Diponegoro, Indonesia
2Department of Marine Science, Faculty of Fisheries and Marine, Universitas Diponegoro, Indonesia
3Faculty of Science, University of the Ryukyus, Japan
BibTex Citation Data :
@article{IK.IJMS50142, author = {Tamara Kainama and Diah Wijayanti and Agus Sabdono and Dio Dirgantara}, title = {DNA Barcoding and Morphological Characters of Two Trevally Fish species (Caranx Spp.) Collected from Youtefa Bay, Papua, Indonesia}, journal = {ILMU KELAUTAN: Indonesian Journal of Marine Sciences}, volume = {28}, number = {1}, year = {2023}, keywords = {DNA barcode; Caranx spp; morphometry; Youtefa bay; Papua}, abstract = { Trevally fish ( Caranx spp,) or Kuwe fish (local name) are small pelagic fish that are mostly caught in the waters of Youtefa Bay. There are two types of Kuwe fish that are difficult to distinguish due to morphological similarities. These Kuwe fish have no scientific species identification so they have several different local names. Accordingly, it is necessary to identify and find out the proper naming of the two Kuwe fish. DNA barcoding is a technique used to speed up and simplify the process of identifying organisms. Hence, the purpose of this research was to identify the proper scientific name of the two Kuwe species in Youtefa Bay waters, as well as the kinship between these two types by applying a DNA barcoding technique. Nineteen Kuwe fish were sampled at Youtefa Bay, Jayapura, Papua in July 2022. Samples were studied morphologically, then carried directly to the Integrated Laboratory of Diponegoro University for molecular studies. DNA extraction, amplification, and DNA sequencing were carried out to further validate the species’ identification. The results showed that in the initial assessment of the specimens using taxonomic keys, 10 specimens were identified as Caranx sexfasciatus and 9 specimens were identified as Caranx tille . The relationship between length and weight of C. sexfasciatus and C. tille have resulted in b = 2.5754 with R2 = 0.8564 and = 1.7119 with R2 = 0.6705, respectively. All identifications were confirmed as correct based on BLASTn results. The BLAST analysis demonstrated that 10 samples were closely related to C. sexfasciatus (99%-100% in similarity) and 9 samples were similar to C. tille (97%- 99%). The genetic distance between individuals in C. sexfasciatus and C. tille has a value ranging from 0% - 0.2%, and the phylogenetic tree exposed that the two species are included in different clades. This study revealed that there are two species of Kuwe fish ( C. sexfasciatus and C. tille ) in Youtefa Bay, Papua, Indonesia. }, issn = {2406-7598}, pages = {97--104} doi = {10.14710/ik.ijms.28.1.97-104}, url = {https://ejournal.undip.ac.id/index.php/ijms/article/view/50142} }
Refworks Citation Data :
Trevally fish (Caranx spp,) or Kuwe fish (local name) are small pelagic fish that are mostly caught in the waters of Youtefa Bay. There are two types of Kuwe fish that are difficult to distinguish due to morphological similarities. These Kuwe fish have no scientific species identification so they have several different local names. Accordingly, it is necessary to identify and find out the proper naming of the two Kuwe fish. DNA barcoding is a technique used to speed up and simplify the process of identifying organisms. Hence, the purpose of this research was to identify the proper scientific name of the two Kuwe species in Youtefa Bay waters, as well as the kinship between these two types by applying a DNA barcoding technique. Nineteen Kuwe fish were sampled at Youtefa Bay, Jayapura, Papua in July 2022. Samples were studied morphologically, then carried directly to the Integrated Laboratory of Diponegoro University for molecular studies. DNA extraction, amplification, and DNA sequencing were carried out to further validate the species’ identification. The results showed that in the initial assessment of the specimens using taxonomic keys, 10 specimens were identified as Caranx sexfasciatus and 9 specimens were identified as Caranx tille. The relationship between length and weight of C. sexfasciatus and C. tille have resulted in b = 2.5754 with R2 = 0.8564 and = 1.7119 with R2 = 0.6705, respectively. All identifications were confirmed as correct based on BLASTn results. The BLAST analysis demonstrated that 10 samples were closely related to C. sexfasciatus (99%-100% in similarity) and 9 samples were similar to C. tille (97%- 99%). The genetic distance between individuals in C. sexfasciatus and C. tille has a value ranging from 0% - 0.2%, and the phylogenetic tree exposed that the two species are included in different clades. This study revealed that there are two species of Kuwe fish (C. sexfasciatus and C. tille) in Youtefa Bay, Papua, Indonesia.
Note: This article has supplementary file(s).
Article Metrics:
Last update:
Last update: 2024-12-22 13:19:17
Copy this form and after filling it, please send it to ijms@live.undip.ac.id:
COPYRIGHT TRANSFER STATEMENT
When this article is accepted for publication, its copyright is transferred to ILMU KELAUTAN Indonesian Journal of Marine Sciences, UNDIP. The copyright transfer covers the non exclusive right to reproduce and distribute the article, including reprints, translations, photographic reproductions, microform, electronic form (offline, online) or any other reproductions of similar nature.
The author warrants that this article is original and that the author has full power to publish. The author signs for and accepts responsibility for releasing this material on behalf of any and all co-authors. In regard to all kind of plagiarism in this manuscript, if any, only the author(s) will take full responsibility. If the article is based on or part of student’s skripsi, thesis or dissertation, the student needs to sign as his/her agreement that his/her works is going to be published.
Title of article :...........................................................................................................................Name of Author(s) :...........................................................................................................................Author’s signature :...........................................................................................................................Date :...........................................................................................................................
View My Stats