DOI: https://doi.org/10.14710/ijred.4.1.25-31

Direct Ethanol Production from Breadfruit Starch (Artocarpus communis Forst.) by Engineered Simultaneous Saccharification and Fermentation (ESSF) using Microbes Consortium

1Major Program of Biotechnology, Graduate School, Bogor Agricultural University, Darmaga Campus, Bogor 16680,, Indonesia

2Department of Agroindustrial Technology, Faculty of Agricultural Techonology, Bogor Agricultural University, Darmaga Campus, Bogor, 16680, Indonesia

Published: 15 Feb 2015.
Editor(s): H Hadiyanto
Open Access Copyright (c) 2015 The Authors. Published by Centre of Biomass and Renewable Energy (CBIORE)
Creative Commons License This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

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Abstract
Breadfruit (Artocarpus communis Forst.) is one of sources for ethanol production, which has high starch content (89%). Ethanol production from breadfruit starch was conducted by Simultaneous Saccharification and Fermentation (SSF) technology using microbes consortium. The aim of the research was to examine a method to produce ethanol by SSF technology using microbes consortium at high yield and efficiency. The main research consisted of two treatments, namely normal SSF and enginereed SSF. The results showed that normal SSF using aeration and agitation during cultivation could produce ethanol at 11.15 ± 0.18 g/L, with the yield of product (Yp/s) 0.34 g ethanol/g substrate; and yield of biomass (Yx/s) 0.29 g cell/g substrate, respectively. A better result was obtained using engineered SSF in which aeration was stopped after biomass condition has reached the end of the exponential phase. The ethanol produced was 12.75 ± 0.04 g/L, with the yields of product (Yp/s) 0.41 g ethanol/g substrate, and the yield of cell (Yx/s) 0.09 g cell/g substrate.
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Keywords: starch, breadfruit, Engineered Simultaneous Saccharification and Fermentation (ESSF), Microbes Consortium, bioethanol

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Section: Original Research Article
Language : EN
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  1. Adepeju AB, Gbadamosi SO, Adeniran AH and Omobuwajo TO. 2011. Fuctional and pasting characteristics of breadfruit (Artocarpus artilis) flours. African Journal of Food Science, Vol. 5(9), pp. 529-535, 15 September 2011
  2. Adinugraha HA and NK Kartikawati. 2004. Pertumbuhan Bibit Tanaman Sukun (Arthocarpus altilis) Hasil Perbanyakan secara Klonal di Persemaian. Prosiding Ekspose Hasil Litbang Bioteknologi dan Pemuliaan Tanaman Hutan. Jogjakarta
  3. Akubor PI, Isolokwu PC, Ugbane O, Onimawo IA. 2000. Proximate composition and functional properties of African breadfruit kernel and flour blends. Food Res. Int., 33: 707-712
  4. AOAC. 1995. Official methods of analysis of the association of official of analytical chemist. Washington
  5. Azmi AS, Hasan M, and Mei M. 2008. Screening of microbes for producing ethanol from cassava starch. 15th Regional Symposium of Malaysian Chemical Engineers (SOMChE), Vol I, pp. 177-181
  6. Azmi A, Hasan M, Mel M, and Ngoh C. 2009. Single-step bioconversion of starch to bioethanol by the coculture of ragi tapai and Saccharomyces cerevisiae. Chemical Engineering Transactions Vol 18. pp 557-562
  7. Balat M, H Balat and C. Oz. 2008. Progress in bioethanol processing. Progress Energy Combustion Sci. 34: 551-573. DOI: 10.1016/J.PECS.2007.11.001
  8. Directorate General of New Renewable Energy and Energy Conservation Ministry of Energy and Mineral Resources. 2013. Standart dan Mutu (Spesifikasi) Bahan Bakar Nabati (Biofuel) Jenis Bioetanol sebagai Bahan Bakar Lain yang Dipasarkan di dalam Negeri. Nomor 722 k/ 10/ DJE/2013.Available http://www.ebtke.esdm.go.id/id/download/doc_download/ 538-kepdirjen-standar-dan-mutu-bbn-jenis-bioetanol.html
  9. Djien KS. 1972. Tape fermentation. Applied Microbiology, 23(5): 976 – 978
  10. Dwidjoseputro D and FT Wolf. 1970. Microbiological studies of Indonesian fermented foodstruffs. Mycopathol. mycol. Appl. 41:211-222
  11. Gandjar I. 2003. Tapai from cassava and cereals. The first international symposium and workshop on insight into the world of indigeneous fermented foods for technology development and food safety, Kasetsart University
  12. Griffin HD. 1981. Fungal physiology. New York: John Wiley and Sons, Inc
  13. Graham HD and de Bravo EN. 1981. Composition of the Breadfruit. J Food Sci 46: 535-539
  14. Imam T and Capareda S. 2011. Fermentation kinetics and ethanol production from different sweet sorgum varieties. Int J Agric & Biol Eng Vol. 4 Number 3
  15. Jenie BS, Putra R, and Feri K. 2012. Fermentasi kultur campuran bakteri asam laktat dan pemanasan autoklaf dalam meningkatkan kadar pati resisten dan sifat fungsional tepung pisang tanduk (Musa paradisiacal formatypica). Jurnal pascapanen 9 (1) 2012: 18-26. Institut Pertanian Bogor
  16. Kunkee KD dan CJ Mardon. 1970. Yeast in wine making. Academic Press, London
  17. Loebis EH. 2008. Optimasi proses hidrolisis kimiawi dan enzimatis tandan kosong kelapa sawit menjadi glukosa untuk produksi etanol. [Thesis]. Bogor: Bogor Agricultural University
  18. Loos PJ, Hood LF, and Graham HD. 1981. Isolation and Characterization of Starch from Breadfruit. Cereal Chem. 58(4): 282 – 286. Department and Institute of Science, Cornell University, Ithaca, NY 14853
  19. Merican Z and Quee–Lan Y. 2004. Tapi processing in Malaysia: A techonology in transition. Industrialization of Indigeneous fermented foods, pp. 247-270, Marcel Dekker Inc., New York
  20. Nadir NM, Karim and RM Yunus, 2009. Comparison of sweet sorghum and cassava for ethanol production by using Saccharomyces cerevisiae. J. Applied Sci., 9: 3068-3073. DOI: 10.3923/jas.2009.3068.3073
  21. Pangloli. (1992). Potensi dan Pemanfaatan Sagu. Kanisius Press. 140p
  22. Prescott SC and Dunn. 1981. Industrial Micrology. Mc Graw Hill Book Co. Ltd, NewYork
  23. Ratledge C and Kristansen Bjorn. 2001. Basic Biotechnology. Second Edition. Published by the press syndicate of the university of Cambridge. United Kingdom (US)
  24. Rehm HJ and Reed G 1983. Biotechnology Vol III. Industrial Microbiology. AVI Publishing Company Inc. Wstport, Connecticut
  25. Saifuddin N and Hussain R. 2011. Microwave Assisted Bioethanol Production from Sago Starch by Co-Culturing of Ragi Tapai and Saccharomyces cerevisiae. Journal of Mathematics and Statistics 7 (3): 198-206
  26. Sattler L dan FW Zerban. 1948. The Dreywood anthrone reaction as affected by carbohydrate structure, Science, 108:207
  27. Steve U, J Osuntogun, and Bohanle A. 1995. Starch. Volume 47, Issue 8, pages 289-294. Wiley-VCH Verlag GmbH& Co. KGaA, Weinheim
  28. Subekti H. 2006. Produksi Etanol Dari Hidrolisat Fraksi Selulosa Tongkol Jagung Oleh Saccharomyces cereviseae. [Essay]. Bogor: Bogor Agricultural University
  29. Supatmawati. 2010. Rekayasa bioproses produksi bioetanol dari hidrolisat pati sagu (Metroxylon sp.) menggunakan Saccharomyces cerevisiae var. ellipsoids pada kultivasi nir-sinambung dan semi sinambung. [Thesis] Bogor Agricultural University: Bogor
  30. Surya L. 2010. Produksi Bioetanol dari Limbah Tanaman Jagung melalui Sakarifikasi dan fermentasi simultan menggunakan biakan Zymomonas mobilis dan Pichia stipitis. [Essay]. Bogor: Bogor Agricultural University
  31. Syamsu K. 2008. Rekayasa bioproses produksi bioetanol dari hidrolisat pati ubi jalar (Ipomea batatas L.) menggunakan Saccharomyces cerevisiae. Laporan Badan Penelitian dan Pengembangan Departemen Penelitian. Bogor Agricultural University. pp 78
  32. Szymanowska D and Grajek W. 2011. Energy-saving and by-products-free production of ethanol from granular corn starch. BioTechnologia vol. 92(1).pp 85-91.2011
  33. Underkofler LA and RJ Hickey. 1954. Industrial fermentation. Chemical Publishing Co, New York
  34. Winarno FG. 1997. Kimia Pangan dan Gizi. PT. Gramedia Pustaka Utama, Jakarta

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