Pengaruh Lidokain Intravena Terhadap Kadar Superoxide Dismutase 1 (Sod-1) Paru Kelinci Dengan Lung Ischemic Reperfusion Injury Model

*Mohammad Arief Kurniawan -  Bagian Anestesi dan Terapi Intensif, Fakultas Kedokteran, Universitas Diponegoro/ RSUP Dr. Kariadi Semarang, Indonesia
Johan Arifin -  Bagian Anestesi dan Terapi Intensif, Fakultas Kedokteran, Universitas Diponegoro/ RSUP Dr. Kariadi Semarang, Indonesia
Taufik Eko Nugroho -  Bagian Anestesi dan Terapi Intensif, Fakultas Kedokteran, Universitas Diponegoro/ RSUP Dr. Kariadi Semarang, Indonesia
Published: 1 Nov 2015.
Open Access
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Article Info
Section: Penelitian
Language: ID
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In Vol 7, No 3 (2015): Jurnal Anestesiologi Indonesia
Statistics: 408 93
Abstract

Latar belakang : Angka kejadian komplikasi paru paska operasi non jantung dibandingkan dengan komplikasi jantung yaitu 2,7% dan 2,5%. Penyebab hal ini adalah stres oksidatif, ketidakseimbangan radikal oksigen dan endogenous scavenging system.Lidokain  menghambat saluran natrium dan, mengurangi masukan kalsium intraseluler, mengurangi produksi Reactive Oxygen Species (ROS) dan modulasi bioenergetika mitokondria, sehingga diharapkan lidokain mampu meningkatkan kadar antioksidan alami di dalam sel.Superoxide Dismutase-1 (SOD-1) adalah salah satu antioksidan alami didalam sel yang berperan dalam melindungi organ dari anion superoksida yang berbahaya dengan mengubah anion yang dihasilkan dari cedera setelah ischaemia-reperfusion.

Tujuan : Mengetahui efek lidokain intravena terhadap kadar Superoxide Dismutase 1 (SOD-1) paru kelinci dengan lung ischemic reperfusion injury model.

Metode : Desain eksperimental laboratorik, 16 kelinci dibagi menjadi dua kelompok secara acak. Kelompok kontrol mendapat perlakuan lung ischemic reperfusion injury dan kelompok perlakuan dilakukan lung ischemic reperfusion injurydan mendapat injeksi lidokain 1,5mg/kgBB/jam intravena secara kontinyu kemudian diukur kadar SOD-1 jaringan paru kedua kelompok. Uji normalitas menggunakan uji Shapiro Wilk dilanjutkan uji beda Independent T-test.

Hasil : Kadar SOD-1 paru kelinci dengan lung ischemic reperfusion injurydan mendapat lidokain lebih tinggi secara signifikan (p=0,01) dibandingkan dengan kadar SOD-1 paru kelinci dengan lung ischemic reperfusion injury

Kesimpulan : Pemberian lidokain kontinyu intravena dapat meningkatkan kadar SOD-1 paru kelinci dengan lung ischemic reperfusion injury.

 

Keywords
Lung ischemic reperfusion injury; Lidokain; SOD-1

Article Metrics:

  1. Smetana G. Postoperative Pulmonary Complications: an Update on Risk Assessment and Reduction. Cleve Clin J Med 2009; 76: 60–5
  2. Kilpatrick B, Slinger P. Lung Protective Strategies in Anaesthesia. BJA. 2010; 105(51):108-16
  3. Ng CSH, Wan S, Yim APC, Arifi AA. Pulmonary Dysfunction After Cardiac Surgery. Chest 2002; 121:1269–77
  4. Wan S, Yim APC, Ng CSH, Arifi AA. Systematic Organ Protection in Coronary Artery Surgery With or Without Cardiopulmonary Bypass. J Card Surg. 2002; 17: 529–35
  5. Ondiveeran HK, Robichaud AF. New Developments in The Treatment of Ischemia/Reperfusion Injury. Current opinion in investigational drugs. 2001; 2(6): 783-91
  6. Fadel E, Mazmanian GM. Lung Reperfusion Injury After Chronic or Acute Unilateral Pulmonary Artery Occlusion. Am J Respir Crit Care Med. 2008; 157:1294-300
  7. Eltzshig H.K, Collard C.D. Vascular Ischaemia and Reperfusion Injury. British medical bulletin. 2004;70:71-76
  8. Vladisvala S. Oxidative Stress in Critical Illnes, Effect of Cytosolic Superoxide Dismutase Over-Expression on Oxidative Stress and Organ Function During Septic Shock. PhD [disertasi]; Michigan University;2006.
  9. K.E. de Greef, D.K. Ysebaert, M. Ghielli et al. Neutrophils and Acute Ischaemia-Reperfusion Injury. J Nephr.1998;13(3):110-22
  10. S. Oredsson, G. Plate and P. Ovarfordt. Experimental evaluation of oxygen free radical scavengers in the prevention of reperfusion injury in skeletal muscle. EJS.1994;160(2):97-103
  11. Durieux M. E, Hollmann M.W. Perioperative Local Aneaesthetic and The Inflamatory Response Administration. European Society of anaesthesiologists. 2004; 107-10
  12. Lee E.H, Lee H.M. Impact of Intravenous Lidocaine on Myocardial Injury After Off-Pump Coronary Arterry Surgery. BJA. 2011; 10 (1093): 1-7
  13. Eroglu A. The Effect of Intravenous Anesthetics on Ischemia-Reperfusion Injury. BioMed Research International.2014;1-7
  14. Ebel D, Lipfert P, Frassdorf J, Preckel B, Mullenheim J, Thamer V et al. Lidocain Reduces Ischaemic but Not Reperfusion Injury in Isolated Rat Heart. British journal of anaesthesia. 2001; 86(6):846-52
  15. Schmid R.A, Yamashita M, Ando K, Tanaka Y, Cooper JD, Patterson GA. Lidocaine Reduces Reperfusion Injury and Neutrophil Migration in Canine Lung Allografts. Ann thorac Surg. 1996; 61:949-55
  16. Imaizumi U, Furuya M, Itakura S, et al. Effect of Lidocaine on Ischemia/Reperfusion Injury in In Vivo Rabbit Hearts. J Anest Clin Res. 2012; 3:11
  17. Festing M. How to Reduce the Number of Animals Used in Research by Improving Experimental Design and Statistics.ANZCCART Fact Sheet. 2011
  18. Kennedy TP, Rao, NV, Hopkins C, Pennington L, Tolley E, Hoidal JR. Role Reactivee Oxygen Species in Reperfuion Injury of tehe Rabbit Lung. J Clin Invest. 2009; 83:1326-35
  19. Draenert A, Marquardt K, Inci I, Soeltermann A. Ischaemia-Reperfusion Injury in Orthopic Mouse Lung Transplants – A Scanning Electron Microscopy Study. Int. J. Exp. Path. 2011; 92:18-25
  20. Unal Y, Iriz E, Yilmazer D, Kavutcu M, Alper M, Kurtipek O, Pekbay A. Effect of Lidocaine on Membrane Stabilization in Harvested Vein Graft Storage. Saudi med J. 2009; 30(2): 203-10
  21. Almaraz CC, Pinilla PJG, Pozo MJ, Camello PJ. Cell Physiology: Mitochondrial Reactive Oxygen Species And Ca2+ Signaling. American Journal of Physiology, 2006;291(5): 1082-88
  22. Arakawa K, Takahashi H, Nakagawa S, Ogawa S. The Effect Of Lidocaine On Superoxide Production And p47 Phox Translocation In Opsonized Zymosan-Activated Neutrophils. Anesth Analg.2001;93:1501-6
  23. Toyokuni S. Reactive Oxygen Species-Induced Molecular Damage and Its Application in Pathology. Pathol Int. 1999; 49: 91–102
  24. Fisher AB, Dodia C, Tan ZT, Ayene I, Eckenhoff RG. Oxygen Dependent Lipid Peroxidation During Lung Ischemia. J ClinInvest.2001;88:674–79
  25. Ambrosio G, Tritto I. Reperfusion Injury: Experimental Evidence and Clinical Implications. Am Heart J.2009;138: S69–S75

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