skip to main content

Antibacterial potential of red dragon fruit peel yogurt (hylocereus spp.) against bacillus subtilis bacteria in hypercholesterolemic wistar rats

1Nutrition, Public Health Science Department, Sports Science Faculty, Universitas Negeri Semarang, Indonesia

2Public Health Science Department, Sports Science Faculty, Universitas Negeri Semarang, Indonesia

Received: 15 Aug 2020; Published: 30 Jun 2021.

Citation Format:
Abstract

Background: Fruit peel is a part of red dragon fruit that weighed 30-35% of the fruit weight and has not been used optimally. Red dragon fruit peel contains fiber, vitamin, flavonoid, tannin, alkaloids and has the potential as an antibacterial. Red dragon fruit peel can be processed into yogurt.

Objectives: This research examined the antibacterial potential of red dragon fruit peel yogurt against Bacillus subtilis in hypercholesterolemic Wistar rats.

Materials and Methods: Materials tested in this study were negative control, positive control, and caecum of hypercholesterolemic Wistar. This study used chloramphenicol as the positive control (K+) and DMSO 10% as the negative control (K-). The K1; K2; K3 were orally administered with 1.8 mL; 2.7 mL; 3.6 mL of red dragon fruit peel yogurt, respectively. Red dragon fruit peel yogurt was administered daily for 28 days. Caecum was collected and tested for antibacterial activity using disk diffusion (Kirby Bauer). The Bacillus subtilis was obtained from the Microbiology Laboratory of Center for Food and Nutrition Studies Universitas Gadjah Mada.

Results: The average inhibition zone in K-; K+; K1; K2; K3 were 0.00±0.00 mm; 11.5±1.41 mm; 11.5±0.96 mm; 10.13±0.66 mm; 10.38±1.12 mm, respectively. The experimental animal groups, which received 2.7 mL and 1.8 mL of red dragon fruit peel yogurts, showed a significant difference compared to the positive control group (p= 0.026 and p=0.021, respectively). When the dose was increased to 3.6 mL, it showed no statistical difference in results (p=1.000).

Conclusions: Red dragon fruit peel yogurt has an antibacterial potential against Bacillus subtilis.

Note: This article has supplementary file(s).

Fulltext View|Download |  common.other
Surat Rekomendasi Artikel dari Panitia ISPHE 2020 (FIK UNNES)
Subject
Type Other
  Download (503KB)    Indexing metadata
 common.other
Surat Pernyataan Persetujuan Penulis
Subject
Type Other
  Download (604KB)    Indexing metadata
Keywords: Antibacterial; Bacillus subtilis; Caecum; Red dragon fruit peel; Yogurt.

Article Metrics:

  1. Waglechner, N. & Wright, G. D. Antibiotic resistance : it’s bad, but why isn't ’ t it worse ? BMC Biology. 2017; 15(84): 1–8
  2. Davies, J. & Davies, D. Origins and Evolution of Antibiotic Resistance. Microbiol. Mol. Biol. Rev. 2010; 74 (3): 417–433
  3. Earl, A. M., Losick, R. & Kolter, R. Ecology and Genomics of Bacillus subtilis. Trends Microbiol. 2008; 16(6): 1–11
  4. Rahayu, W. P. & Nurwitri, C. Mikrobiologi Pangan. IPB Press; 2012
  5. Jaafar, R. A., Ridhwan, A., Mahmod, N. Z. C. & Vasudevan, R. Proximate Analysis of Dragon Fruit ( Hylocereus polyhizus ). Am. J. Appl. Sci. 2009; 6(7): 1341–1346
  6. Jaya, I. K. D. Morphology And Physiology Of Pitaya And It Future Prospects In Indonesia. Crop Agro 2010; 3 (1): 44–50
  7. Nurliyana, R., Zahir, S., Suleiman, M., Aisyah, M. & Rahim, K. Antioxidant study of pulps and peels of dragon fruits : a comparative study. International Food Research Journal. 2010; 17: 367–375
  8. Amalia, S., Wahdaningsih, S. & Untari, E. K. Antibacterial Activity Testing Of N-Hexane Fraction Of Red Dragon ( Hylocereus polyrhizus Britton & Rose ) Fruit Peel On Staphylococcus aureus ATCC 25923. Traditional Medicine Journal 2014; 19(2): 89–94
  9. Fitratullah, A., Maruddin, F., Yuliati, F., Prahesti, K. & Taufik, M. Addition of red dragon fruit ( Hylocereus polyrhizus ) on yogurt : Effect on lactic acid content, pH, and the inhibition of Escherichia coli growth. in IOP Conf. Series: Earth and Environmental Science 2019; 343: 1–7 (IOP Publishing). doi: 10.1088/1755-1315/343/1/012034
  10. Maulana, I. et al. Antibacterial Test of Red Dragon Fruit Extract Peel ( Hylocereus polyrhizus ) Against Bacteria Salmonella pullorum. Jurnal Medika Veterinaria 2018; 12 (1): 9–14
  11. Septiana, W. C. & Ardiaria, M. Efek Pemberian Seduhan Kulit Buah Naga Merah (Hylocereus polyrhizus) terhadap Kadar Malondialdehyde (MDA) Tikus Sprague Dawley Dislipidemia. Journal of Nutrition College 2017; 5 (4): 344–352
  12. Mardiana, Budiono, I. & Putriningtyas, N. D. Comparison of organoleptic, protein, lipid and flavonoid content of commercial starter and isolated culture red dragon fruit peel yogurt. Food Research. 2020: 4(3): 920–925
  13. Ayala, F. R. et al. Microbial flora, probiotics, Bacillus subtilis and the search for a long and healthy human longevity. Microbial Cell 2017; 4 (4): 133–136
  14. Murase, T., Yamada, N. & Uchimura, H. Accumulation of intermediate-density lipoprotein in the plasma of cholesterol-fed hypothyroid rats. Metabolism 1983; 32 (2): 146–150
  15. Putriningtyas, N. D. & Wahyuningsih, S. Potensi yogurt kacang merah (Phaseolus vulgaris L) ditinjau dari sifat organoleptik, kandungan protein, lemak dan flavonoid. Jurnal Gizi Indonesia (The Indonesian Journal of Nutrition). 2017; 6 (1): 37–43
  16. Prayoga, E. Perbandingan Efek Ekstrak Daun Sirih Hijau (Piper betle L.) dengan Metode Difusi Disk dan Sumuran terhadap Pertumbuhan Bakteri Staphylococcus aureus. [Tesis]. Jakarta: Universitas Islam Negeri Syarif Hidayatullah; 2013. doi: 10.1023/B:FOOP.0000019620.04821.a2
  17. Maruddin, F., Malaka, R. & Taufi, M. Characteristics and antimicrobial activity of dangke whey fermentation with sugar addition. Bulgarian Journal of Agricultural Science. 2019; 25 (2): 410–417
  18. Octaviani, M., Fadhli, H. & Yuneistya, E. Uji Aktivitas Antimikroba Ekstrak Etanol dari Kulit Bawang Merah (Allium cepa L.) dengan Metode Difusi Cakram. Pharm. Sci. Res. 2019; 6 (1): 62–68
  19. Fitriani, E. Uji Aktivitas Antibakteri Ekstrak Etanol Daun Sirsak (Annona muricata L.) terhadap Shigella flexneri Secara In Vitro. [Tesis]. Pontianak: Universitas Tanjungpura; 2014
  20. Ritarwan, K. & Nerdy, N. Antibacterial Activity of Red Dragon Fruit Leaves Extract and White Dragon Fruit Leaves Extract Against Meningitis Bacterial. Orient. J. Chem. 2018; 34 (5): 2534–2538
  21. Sah, B. N. P., Vasiljevic, T., Mckechnie, S. & Donkor, O. N. Antibacterial and antiproliferative peptides in synbiotic yogurt — Release and stability during refrigerated storage. J. Dairy Sci. 2016; 99: 4233–4242
  22. Luo, H., Cai, Y., Peng, Z., Liu, T. & Yang, S. Chemical composition and in vitro evaluation of the cytotoxic and antioxidant activities of supercritical carbon dioxide extracts of pitaya (dragon fruit) peel. Chemistry Central Journal. 2014; 8 (1): 1–7
  23. Karimi, S., Azizi, F., Nayeb-aghaee, M. & Mahmoodnia, L. The Antimicrobial Activity of Probiotic Bacteria Escherichia coli Isolated from Different Natural Sources Against Hemorrhagic E. coli O157:H7. Electron. Physician 2018; 10 (3): 6548–6553
  24. Choo, K. Y., Kho C., Ong, Y. Y., Thoo, Y.Y., Lim, R.L. H., Tan, C.P., et al. Studies on the storage stability of fermented red dragon fruit (Hylocereus polyrhizus) drink. Food Sci. Biotechnol. 2018; 27: 1411–1417
  25. Wahdaningsih, S., Untari, E. K. & Fauziah, Y. Antibakteri Fraksi n-Heksana Kulit Hylocereus polyrhizus Terhadap Staphylococcus epidermidis dan Propionibacterium acnes. Pharm. Sci. Res. 2014; 1 (3): 180–193
  26. Astridwiyanti, A. A. B., Mahendra, A. N. & Dewi, N. W. S. Uji efektivitas ekstrak etanol kulit buah naga merah (Hylocereus polyrhizus) terhadap Staphylococcus aureus ATCC 25923 secara in vitro. Intisari Sains Medis 2019; 10 (3): 482–486

Last update:

No citation recorded.

Last update: 2024-11-20 13:40:57

No citation recorded.