Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University, Indonesia
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@article{JKSA15204, author = {Maulida Ratri and Purbowatiningrum Sarjono and Agustina Aminin}, title = {Studi Filogeni dan Uji Potensi Bioremediasi serta Enzim Termostabil Ekstraseluler Isolat Geobacillus sp. dari Sumber Air Panas Gedong Songo}, journal = {Jurnal Kimia Sains dan Aplikasi}, volume = {12}, number = {2}, year = {2009}, keywords = {Mikroorganisme termofilik; Geobacillus sp; bioremediasi}, abstract = {Mikroorganisme termofilik merupakan mikroorganisme yang tahan terhadap suhu tinggi dengan suhu optimum pertumbuhan mencapai lebih dari 60°C. Mikroorganisme termofilik mempunyai banyak kegunaan, antara lain untuk proses bioremediasi dan penghasil enzim termostabil. Penelitian ini bertujuan untuk menelusuri potensi Geobacillus sp. dari sumber air panas Gedong Songo melalui konstruksi pohon filogeni serta mendapatkan data potensi bioremediasi Fe(III), Pb(II), H2O2 dan enzim ekstraselular dari isolat Geobacillus sp. Konstruksi pohon filogeni dilakukan menggunakan program Phylip 3.68 ed dengan metode parsimony. Uji potensi Geobacillus sp. untuk bioremediasi Fe(III) dan Pb(II) dilakukan dengan menginokulasikan pellet Geobacillus sp. pada media BSM (Basal Salin Medium) yang mengandung masing-masing Fe(III) dan Pb(II). Analisis kadar Fe(III) dan Pb(II) pada media dilakukan dengan AAS. Uji potensi bioremediasi H2O2 dilakukan secara kualitatif dengan melihat adanya gelembung O2 yang dihasilkan oleh aktifitas katalase dari Geobacillus sp. Uji aktivitas enzim amilase, β-galaktosidase dan protease ekstraseluler dilakukan secara kualitatif dengan penambahan masing-masing amilum, ONPG dan gelatin sebagai substratnya. Penelitian menunjukkan hasil positif pada uji potensi bioremediasi Fe(III) dan Pb(II) serta untuk uji enzim amilase dan protease ekstraseluler. Uji potensi bioremediasi H2O2 dan enzim β-galaktosidase ektraseluler menunjukkan hasil negatif. Konstruksi pohon filogeni menunjukkan bahwa kedelapan isolat Geobacillus sp. memiliki kekerabatan terdekat dengan Geobacillus sp._TERI_NSM dan Geobacillus sp. sbs3.}, issn = {2597-9914}, pages = {31--39} doi = {10.14710/jksa.12.2.31-39}, url = {https://ejournal.undip.ac.id/index.php/ksa/article/view/15204} }
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