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Antioxidant Activity of Daemonorops draco Resin

1Department of Chemistry, Faculty of Mathematics and Natural Sciences, IPB University, Indonesia

2Department of Chemistry, Faculty of Mathematics and Natural Science, IPB University, Indonesia

Received: 6 May 2019; Revised: 5 Aug 2019; Accepted: 13 Sep 2019; Published: 30 Sep 2019.
Open Access Copyright 2019 Jurnal Kimia Sains dan Aplikasi under

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Jernang resin is secretion of jernang rattan (Daemonorops draco, Arecaceae family) fruits which is endemic in Southeast Asia. This resin has various biological activities and empirically used as wound healing, headache medicines, and fever remedies by Anak Dalam ethnic group from Jambi. This study was performed to evaluate the antioxidant activity of nonpolar fraction of D. draco resin which collected from Jambi Province, Sumatera, Indonesia. Resin was extracted with n-hexane, ethyl acetate, and methanol respectively. The antioxidant properties of the extracts were then evaluated using 1,1-diphenyl-2picryl-hidrazyl radical scavenging assay. The most active extract was further fractionated using n-hexane and methanol and separated using column chromatography and preparative thin layer chromatography. Separation of the extract was conducted through antioxidant assay-guided fractionation. Characterization of the active fraction was carried out by infrared spectroscopy. The result shows that ethyl acetate extract provides higher antioxidant activity (IC50 = 27.61 µg/mL) compare to methanol and n-hexane extracts. N-hexane fraction of ethyl acetate extract used for further separation using column and preparative thin layer chromatography due to its antioxidant activity. Separation using column chromatography resulting in 9 fractions (F.1-9). Fraction F.5 provide high antioxidant activity (IC50 = 17.27 µg/mL) and further separated using preparative thin layer chromatography resulting two fractions with lower antioxidant activity F.5.1 (IC50 = 85.18 µg/mL) and F.5.2 (IC50 = 34.94 µg/mL). Characterization of fraction F.5.2 using infrared spectroscopy showed that component in fraction F.5.2 contains NH-substituted benzene.

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Keywords: Antioxidant; chromatography; Daemonorops draco; fractionation; resin
Funding: Ministry of Research, Technology and Higher Education in accordance with the Letter of Appointment Implementation of Research Program Number: 079/SP2H/LT/DRPM/ II/2016 17 February 2016

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Section: Research Articles
Language : EN
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  2. Vuokko L. Kinnula and James D. Crapo, Superoxide dismutases in malignant cells and human tumors, Free Radical Biology and Medicine, 36, 6, (2004) 718-744
  3. Uma Singh and Ishwarlal Jialal, Oxidative stress and atherosclerosis, Pathophysiology, 13, 3, (2006) 129-142
  4. Mark A. Smith, Catherine A. Rottkamp, Akihiko Nunomura, Arun K. Raina and George Perry, Oxidative stress in Alzheimer’s disease, Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, 1502, 1, (2000) 139-144
  5. Termentzi Aikaterini, Fokialakis Nikolas and Skaltsounis Alexios Leandros, Natural Resins and Bioactive Natural Products thereof as Potential Anitimicrobial Agents, Current Pharmaceutical Design, 17, 13, (2011) 1267-1290
  6. Mardho Tillah, Irmanida Batubara and Rita Kartika Sari, Antimicrobial and Antioxidant Activities of Resins and Essential Oil From Pine (Pinus merkusii, Pinuso ocarpa, Pinus insularis) and Agathis (Agathis loranthifolia), Biosaintifika: Journal of Biology & Biology Education, 9, 1, (2017) 134-139
  7. Deepika Gupta and Rajinder K. Gupta, Bioprotective properties of Dragon's blood resin: In vitro evaluation of antioxidant activity and antimicrobial activity, BMC Complementary and Alternative Medicine, 11, 1, (2011) 1-9
  8. Deepika Gupta, Bruce Bleakley and Rajinder K. Gupta, Dragon's blood: Botany, chemistry and therapeutic uses, Journal of Ethnopharmacology, 115, 3, (2008) 361-380
  9. Rana Rio Andhika, Bambang Hariyadi and Fachruddin Saudagar, Etnobotani Penghasil Getah oleh Suku Anak Dalam di Taman Nasional Bukit Duabelas Kabupaten Sarolangun, Jambi, Jurnal Ilmu Pertanian Indonesia, 20, 1, (2015) 33-38
  10. G. S. R. Rao, M. A. Gerhart, R. T. Lee, L. A. Mitscher and S. Drake, Antimicrobial Agents From Higher Plants. Dragon's Blood Resin, Journal of Natural Products, 45, 5, (1982) 646-648
  11. Wulan Tri Wahyuni, Sri Purwanti and Irmanida Batubara, Antibacterial and Antibiofilm Activity of Daemonorops draco Resin, Biosaintifika: Journal of Biology & Biology Education, 10, 1, (2018) 138-144
  12. Jing-hua Yu, Gui-bin Zheng, Chun-yu Liu, Li-ying Zhang, Hong-mei Gao, Ya-hong Zhang, Chun-yan Dai, Lin Huang, Xian-ying Meng, Wen-yan Zhang and Xiao-fang Yu, Dracorhodin Perchlorate Induced Human Breast Cancer MCF-7 Apoptosis through Mitochondrial Pathways, International Journal of Medical Science, 10, 9, (2013) 1149-1156
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  14. Qian Hao, Yoshinori Saito, Yosuke Matsuo, Hai-Zhou Li and Tanaka Takashi, Three new flavans in dragon's blood from Daemonorops draco, Natural Product Research, 29, 15, (2015) 1419-1425
  15. Alberto Arnone, Gianluca Nasini, Orso Vajna de Pava and Lucio Merlini, Constituents of Dragon's Blood. 5. Dracoflavans B1, B2, C1, C2, D1, and D2, New A-Type Deoxyproanthocyanidins, Journal of Natural Products, 60, 10, (1997) 971-975
  16. Ken-ichi Nakashima, Naohito Abe, Fumiko Kamiya, Tetsuro Ito, Masayoshi Oyama and Munekazu Iinuma, Novel Flavonoids in Dragon's Blood of Daemonorops draco, Helvetica Chimica Acta, 92, 10, (2009) 1999-2008
  17. Irmanida Batubara, Tohru Mitsunaga and Hideo Ohashi, Screening antiacne potency of Indonesian medicinal plants: antibacterial, lipase inhibition, and antioxidant activities, Journal of Wood Science, 55, 3, (2009) 230-235
  18. Bishnu Prasad Pandey, Rupak Thapa and Anil Upreti, Chemical composition, antioxidant and antibacterial activities of essential oil and methanol extract of Artemisia vulgaris and Gaultheria fragrantissima collected from Nepal, Asian Pacific Journal of Tropical Medicine, 10, 10, (2017) 952-959

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