Isolasi Bakteri Endofit pada Rimpang Jahe Merah (Zingiber officinale Linn. Var Rubrum) Penghasil Senyawa Antioksidan

Okky Triana; Purbowatiningrum Ria Sarjono; Nies Suci Mulyani

doi:10.14710/jksa.20.1.25-29

DOI: https://doi.org/10.14710/jksa.20.1.25-29

Isolasi Bakteri Endofit pada Rimpang Jahe Merah (Zingiber officinale Linn. Var Rubrum) Penghasil Senyawa Antioksidan

Okky Triana, Purbowatiningrum Ria Sarjono

, Nies Suci Mulyani

Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University, Indonesia

Published: 1 Apr 2017.

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Abstract

Telah dilakukan penelitian mengenai isolasi bakteri endhofit pada jahe merah (zingiber officinale linn. var rubrum)penghasil senyawa antioksidan. Penelitian ini bertujuan untuk memperoleh isolat bakteri endofit yang bersimbiosis dengan rimpang jahe merah, memperoleh data aktivitas antioksidan dengan metode DPPH dan mengetahui informasi mengenai kandungan kimia metabolit sekunder bakteri endofit. Metode yang digunakan pada penelitian ini adalah surface sterilization dan spreading pada media YMA dilanjutkan dengan uji aktivitas antioksidan dengan metode peredaman radikal DPPH dan penapisan fitokimia. Tahapan penelitian ini meliputi isolasi bakteri endofit yang bersimbiosis dengan rimpang jahe, produksi metabolit sekunder isolat bakteri endofit, uji aktivitas antioksidan dan penapisan fitokimia. Penelitian ini menghasilkan tiga isolat bakteri endofit yaitu isolat J₁, isolat J₂ dan isolat J₃ yang memiliki morfologi sel berbeda serta memiliki aktivitas antioksidan yang paling efektif pada konsentrasi 31,25 ppm dengan nilai % inhibisi pada isolat J₁, J₂ dan J₃ berturut-turut sebesar 22,71% ; 20,86% dan 13,08% dan semua metabolit sekunder isolat bakteri endofit memiliki kandungan flavanoid dan saponin.

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Keywords: Bakteri endofit; Antioksidan; jahe merah; Zingiber officinale Linn. Var rubrum

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Section: Research Articles

Language : ID

In Vol 20, No 1 (2017): Volume 20 Issue 1 Year 2017

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