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Uji Aktivitas Antioksidan Senyawa Flavonoid dari Ekstrak Etanol Daun Johar (Senna siamea Lamk)

Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University, Indonesia

Published: 1 Oct 2017.
Open Access Copyright 2017 Jurnal Kimia Sains dan Aplikasi under http://creativecommons.org/licenses/by-sa/4.0/.

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Abstract
Telah dilakukan penelitian tentang isolasi senyawa flavonoid dari ekstrak etanol daun johar (Senna siamea Lamk) serta uji aktivitas antioksidan. Penelitian ini bertujuan untuk memperoleh isolat flavonoid, mengidentifikasi dan menguji aktivitas antioksidan isolat flavonoid dari ekstrak etanol daun johar. Metode penelitian dibagi menjadi empat tahap, tahap pertama yaitu isolasi senyawa flavonoid. Tahap kedua yaitu pemisahan senyawa flavonoid menggunakan kromatografi cair vakum, kromatografi kolom gravitasi dan kromatografi lapis tipis preparatif. Tahap ketiga yaitu identifikasi isolat flavonoid menggunakan spektrofotometer UV-Vis dan FTIR. Tahap keempat adalah uji aktivitas antioksidan dengan metode DPPH. Hasil isolasi flavonoid didapatkan isolat flavonoid seberat 0,006 gram (0,004%). Identifikasi isolat flavonoid dengan menggunakan spektrofotometer UV-Vis menunjukkan bahwa isolat flavonoid memiliki struktur dasar senyawa flavon dengan serapan maksimum pada panjang gelombang pita I (350 nm) dan pita II (255 nm dan 267 nm) dan analisis menggunakan FTIR menunjukkan isolat flavonoid memiliki gugus fungsi O-H, C-H alifatik, C=O, C=C aromatik, C-O eter dan C-O alkohol sehingga diduga isolat flavonoid memiliki struktur dasar senyawa luteolin yang tersubstitusi. Hasil uji aktivitas antioksidan isolat flavonoid menunjukkan IC50 sebesar 139,8373 mg/L.
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Keywords: antioksidan; flavonoid; ekstrak etanol; daun johar; Senna siamea Lamk

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  1. Mamadou Kamagaté, Camille Koffi, N’goran Mathieu Kouamé, Aminata Akoubet, N’guessan Alain Roland Yao, Henri Maxime Die-Kakou, Ethnobotany, phytochemistry, pharmacology and toxicology profiles of Cassia siamea Lam, The Journal of Phytopharmacology, 3, 1, (2014) 57-76
  2. Lakshmi Narayana Majji, Ganga Rao Battu, Ravi Kumar Jangiti, Mallikarjun Rao Talluri, Evaluation of In-Vitro Antibacterial Activity of Cassia Siamealeaves, International Journal of Pharmacy and Pharmaceutical Sciences, 5, 3, (2013) 263-265
  3. P. Poovendran, N. Ramanathan, N. Prabhu, Evaluation of the Antibacterial Activity of Aegle marmelos and Cassia siamea Extracts Against Biofilm and Extended Spectrum-Lactamase Producing Uropathogenic Escherichia coli, International Journal of Microbiological Research, 5, 3, (2014) 217-221 http://dx.doi.org/10.5829/idosi.ijmr.2014.5.3.9138
  4. S. Kumar, V. Kumar, Om Prakash, Antidiabetic and anti-lipemic effects of Cassia siamea leaves extract in streptozotocin induced diabetic rats, Asian Pacific Journal of Tropical Medicine, 3, 11, (2010) 871-873 https://doi.org/10.1016/S1995-7645(10)60209-X
  5. Usha Veerachari, A. K. Bopaiah, Preliminary phyto-chemical evaluation of the leaf extract of five Cassia Species, Journal of Chemical and Pharmaceutical Research, 3, 5, (2011) 574-583
  6. K. Ingkaninan, A. P. Ijzerman, R. Verpoorte, Luteolin, a Compound with Adenosine A1 Receptor-Binding Activity, and Chromone and Dihydronaphthalenone Constituents from Senna siamea, Journal of Natural Products, 63, 3, (2000) 315-317 http://dx.doi.org/10.1021/np9904152
  7. K. R. Markham, Techniques of flavonoid identification, Academic Press, 1982
  8. Keyong Xu, Benguo Liu, Yuxiang Ma, Jiquan Du, Guanglei Li, Han Gao, Yuan Zhang, Zhengxiang Ning, Physicochemical Properties and Antioxidant Activities of Luteolin-Phospholipid Complex, Molecules, 14, 9, (2009) 3486
  9. Robert Milton Silverstein, Francis X. Webster, David J. Kiemle, Spectrometric Identification of Organic Compounds, John Wiley & Sons, 2005
  10. Amic Dragan, Davidovic-Amic Dusanka, Beslo Drago, Rastija Vesna, Lucic Bono, Trinajstic Nenad, SAR and QSAR of the Antioxidant Activity of Flavonoids, Current Medicinal Chemistry, 14, 7, (2007) 827-845 http://dx.doi.org/10.2174/092986707780090954
  11. Marsden S. Blois, Antioxidant Determinations by the Use of a Stable Free Radical, Nature, 181, (1958) 1199 http://dx.doi.org/10.1038/1811199a0

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