skip to main content

Studi Filogeni dan Uji Potensi Enzim Ekstraseluler (amilase, β-galaktosidase, protease, katalase) Isolat Alicyclobacillus sp. Gedong Songo

Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University, Indonesia

Published: 1 Dec 2010.
Open Access Copyright 2010 Jurnal Kimia Sains dan Aplikasi under http://creativecommons.org/licenses/by-sa/4.0/.

Citation Format:
Abstract
Penelitian terdahulu pada sumber air panas Gedong Songo, telah diisolasi 5 isolat Alicyclobacillus sp. yaitu: ATSae10, ANBae10, AYTae7, AYTae31 dan AYTae33 dengan kondisi pertumbuhan 55°C dan pH 4. Tujuan penelitian ini yaitu untuk mendapatkan pohon filogeni dan mendapatkan informasi mengenai potensi enzim ekstraseluler ketiga isolat Alicyclobacillus sp. yang meliputi amilase, β-galaktosidase, protease dan katalase. Konstruksi pohon filogeni dilakukan menggunakan program Phylip 3.68 ed dengan metode parsimony. Uji potensi enzim ekstraseluler isolat Alicyclobacillus sp. dilakukan secara kualitatif. Uji aktifitas amilase menggunakan amilum sebagai substrat, uji aktifitas β-galaktosidase menggunakan ONPG sebagai substrat dan pada uji protease menggunakan gelatin sebagai substrat. Uji katalase dilakukan secara kualitatif dengan melihat adanya gelembung O2 yang dihasilkan oleh aktifitas katalase. Hasil penelitian menunjukkan bahwa ketiga isolat Alicyclobacillus sp. (AYTae7, AYTae31 dan AYTae33) berada dalam satu kelompok kekerabatan dan mempunyai tingkat kekerabatan terdekat dengan Alicyclobacillus sp. KI, Alicyclobacillus sp. T127, Alicyclobacillus acidocaldarius strain DSM 454 dan Uncultured bacterium clone351. Ketiga isolat Alicyclobacillus sp. memiliki enzim termostabil ekstraseluler amilase dan β-galaktosidase.
Fulltext View|Download
Keywords: Bakteri termofilik; Alicyclobacillus sp.; enzim ekstraseluler

Article Metrics:

  1. Trismilah, Mahyudin A.R, Pemanfaatan Kulit Buah Pisang Nangka Sebagai Substrat Fermentasi Padat Pada Produksi Xilanase, Jurnal Rekayasa Lingkungan, 5, 1, (2009) 13-23
  2. Claudia Schmidt-Dannert, Helena Sztajer, Walter Stöcklein, Ulrich Menge, Rolf D. Schmid, Screening, purification and properties of a thermophilic lipase from Bacillus thermocatenulatus, Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1214, 1, (1994) 43-53 https://doi.org/10.1016/0005-2760(94)90008-6
  3. Agustina L. N. Aminin, Fida Madayanti Warganegara, Pingkan Aditiawati, Akhmaloka, Culture-Independent and culture-dependent approaches on microbial community analysis at Gedongsongo (GS-2) hot spring, International Journal of Integrative Biology, 2, 3, (2008) 145-152
  4. Rudolf I Amann, Wolfgang Ludwig, Karl-Heinz Schleifer, Phylogenetic identification and in situ detection of individual microbial cells without cultivation, Microbiological reviews, 59, 1, (1995) 143-169
  5. Jusuf Ginting, Isolasi Bakteri Dan Uji Aktivitas Enzim Amilase Kasar Termofilik Dari Sumber Air Panas Semangat Gunung Kabupaten Karo, Sumatera Utara, Universitas Sumatera Utara, Medan
  6. Ryan D. Martinus, Graeme P. Garth, Tracie L. Webster, Peter Cartwright, Dean J. Naylor, Peter B. Høj, Nicholas J. Hoogenraad, Selective Induction of Mitochondrial Chaperones in Response to Loss of the Mitochondrial Genome, European Journal of Biochemistry, 240, 1, (1996) 98-103 http://dx.doi.org/10.1111/j.1432-1033.1996.0098h.x
  7. Elidar Naiola, Nunuk Widhyastuti, Semi Purifikasi dan Karakterisasi Enzim Protease Bacillus sp, Berkala Penelitian Hayati, 13, 1, (2007) 51-56
  8. Rosliana Pakpahan, Isolasi Bakteri dan Uji Aktivitas Protease Termofilik dari Sumber Air Panas Sipoholon Tapanuli Utara Sumatera Utara, (2009)
  9. Agustina L. N. Aminin, Fida Madayanti Warganegara, Pingkan Aditiawati, Simple enrichment and independent cultures to expand bacterial community analysis from gedongsongo hot spring, Journal of Bioscience and Bioengineering, 106, 2, (2008) 211-214
  10. E. Stackebrandt, B. M. Goebel, Taxonomic Note: A Place for DNA-DNA Reassociation and 16S rRNA Sequence Analysis in the Present Species Definition in Bacteriology, International Journal of Systematic and Evolutionary Microbiology, 44, 4, (1994) 846-849 http://dx.doi.org/10.1099/00207713-44-4-846
  11. Agustina L. N. Aminin, F. Madayanti, P. Aditiawati, Akhmaloka, Isolation of Thermophiles From Gedongsongo Hot Spring Using A Simple Enrichment Medium, in: International Seminar Advance in Biological Science, 2007
  12. Michael T. Madigan, John M. Martinko, Brock Biology of Microorganisms, 11 ed., Pearson Prentice Hall, 2006
  13. Hans G. Schlegel, General Microbiology, 7 ed., Cambridge University Press, 1986
  14. Agnes Ullmann, Roots: Complementation in β-galactosidase: From protein structure to genetic engineering, BioEssays, 14, 3, (1992) 201-205 http://dx.doi.org/10.1002/bies.950140311
  15. Necmettin Yildirim, Michael C. Mackey, Feedback Regulation in the Lactose Operon: A Mathematical Modeling Study and Comparison with Experimental Data, Biophysical Journal, 84, 5, 2841-2851 http://dx.doi.org/10.1016/S0006-3495(03)70013-7
  16. Sophie Drouault, Jamila Anba, Gérard Corthier, Streptococcus thermophilus is able to produce a β-galactosidase active during its transit in the digestive tract of germ-free mice, Applied and environmental microbiology, 68, 2, (2002) 938-941 http://dx.doi.org/10.1128/AEM.68.2.938-941.2002
  17. Joseph Sambrook, David William Russell, Molecular cloning: a laboratory manual, Coldspring-Harbour Laboratory Press, UK, (2001)
  18. Tuti Susilawati, Isolasi dan karakterisasi enzim protease dari isolat bakteri termofilik sumber air panas Gedong Songo Bawen, Skripsi, Jurusan Kimia, Universitas Diponegoro, Semarang
  19. David M Ward, Diversity, Ecology, and Evolution of Microorganisms Inhabiting Hot Spring Microbial Mats, Journal of NSF Frontiers of Integrative Biologycal Research, (2003)
  20. Ruth Melliawati, Ricky Setiadi Suherman, Bambang Subardjo, Pengkajian Kapang Endofit dari Taman Nasional Gunung Halimun sebagai Penghasil Glukoamilase, Berkala Penelitian Hayati, 12, (2006) 19-25
  21. John M. DeMan, Principles of Food Chemistry, Springer, 1999
  22. L Luhova, A Lebeda, D Hedererová, P Pec, Activities of amine oxidase, peroxidase and catalase in seedlings of Pisum sativum L. under different light conditions, Plant soil and environment, 49, 4, (2003) 151-157
  23. Ventsislava Yankova Petrova, Tanya Vassileva Rasheva, Anna V Kujumdzieva, Catalase enzyme in mitochondria of Saccharomyces cerevisiae, Electronic Journal of Biotechnology, 5, 1, (2002) 11-12
  24. Marini Wijayanti, Produksi, isolasi dan karakterisasi superoksida dismutase dari Spirulina platensis yang dibiakkan dalam serum lateks Production, isolation, and characterization of superoxyde dismutase from Spirulina platensis cultured on latex serum, E-Journal Menara Perkebunan, 77, 1, (2016)

Last update:

  1. Isolation, Characterization, Molecular Identification of Probiotic Bacteria from Meconium

    Dhanly Arie Irza, I Nyoman Ehrich Lister, Saipul Sihotang, Edy Fachrial. IOP Conference Series: Earth and Environmental Science, 755 (1), 2021. doi: 10.1088/1755-1315/755/1/012041

Last update: 2024-11-21 02:10:14

No citation recorded.