DOI: https://doi.org/10.14710/jksa.23.1.1-7

Activity of Actinomycetes Isolated from Mangrove Segara Anakan Cilacap toward Methicillin-resistant Staphylococcus aureus (MRSA)

1Chemistry Department, Faculty of Mathematics and Natural Science, Universitas Jenderal Soedirman, Indonesia

2Biology Department, Faculty of Biology, Universitas Jenderal Soedirman, Indonesia

Received: 28 Aug 2019; Revised: 15 Nov 2019; Accepted: 6 Dec 2019; Available online: 31 Jan 2020; Published: 31 Jan 2020.
Open Access Copyright 2020 Jurnal Kimia Sains dan Aplikasi under http://creativecommons.org/licenses/by-sa/4.0.

Citation Format:
Cover Image
Abstract
Methicillin-resistant Staphylococcus aureus (MRSA) is a major health concern because it causes numerous infections in both healthcare facilities and communities. The development of multiresistant against topical antibiotics has caused substantial difficulty in the management of Staphylococcus infection. Thus, this research was aimed to explore indigenous marine Actinomycetes isolated from Segara Anakan Cilacap for anti-MRSA activity. The methods used were screening anti-MRSA activity using agar-block method, production of anti-MRSA extract, extraction of the anti-MRSA extract with ethyl acetate, MIC determination of the ethyl-acetate extract, and species identification based on morphology and 16S rRNA genes. The results indicated that 14 out of 16 Actinomycetes have anti-MRSA activity. Three isolates, which were W-5B, W-5A, and P-7D, showed the highest anti-MRSA activity with the inhibition zone of 2.40 mm, 1.20 mm, and 0.80 mm, respectively. The minimum inhibitory concentration (MIC) of ethyl acetate extract from isolates W-5B, W-5A, and P-7D against MRSA were 2 mg/mL, 4 mg/mL, and 8 mg/mL, respectively. The species identification based on 16S rRNA genes indicated that Actinomycetes W-5B isolate has 92.51% similarity with Streptomyces sp. 2011. The W-5A has 94.69% similarity with Arthrobacter sp. HZ11. The P-7D has 94.79% similarity with Streptomyces clavuligerus strain A-ZN-05. The present study concludes that marine Actinomycetes from sediment mangrove in Segara Anakan Cilacap, Indonesia, has potency as anti-MRSA.
Fulltext View|Download
Keywords: Actinomycetes; MRSA; Segara Anakan; Streptomyces; 16S rRNA
Funding: Directorate General of Higher Education of Republic of Indonesia for the Basic Fundamental Grants

Article Metrics:

Article Info
Section: 8th - International Conference of the Indonesian Chemical Society (ICICS)
Language : EN
Recent articles
Activity of Actinomycetes Isolated from Mangrove Segara Anakan Cilacap toward Methicillin-resistant Staphylococcus aureus (MRSA) Activation of Natural Zeolite and Its Application for Adsorbents in Domestic Wastewater Treatment in Tembalang District, Semarang City Antiglycation and Antioxidant Activity from Methanol Extract and Fraction of Xylocarpus granatum Stem Cytotoxic, Antiproliferation, and Necrosis Effects of the n-Hexane Fraction Extract of Gendola Leaf (Basella rubra Linn.) More recent articles
Most cited articles
Isolasi Bakteri Endofit pada Rimpang Jahe Merah (Zingiber officinale Linn. Var Rubrum) Penghasil Senyawa Antioksidan Utilization of Rice Husk Cellulose as a Magnetic Nanoparticle Biocomposite Fiber Source for the Absorption of Manganese (Mn2+) Ions in Peat Water Sintesis Asam Eugenoksi Asetat (EOA) dari Eugenol untuk Ekstraktan Logam Berat dan Recovery Krom dari Limbah Elektroplating Activity Test of Suji Leaf Extract (Dracaena angustifolia Roxb.) on in vitro cholesterol lowering Interlayer-free Silica Pectin Membrane for Wetland Saline Water via Pervaporation More cited articles
  1. Kelly M. Craft, Johny M. Nguyen, Lawrence J. Berg and Steven D. Townsend, Methicillin-resistant Staphylococcus aureus (MRSA): antibiotic-resistance and the biofilm phenotype, MedChemComm, 10, 8, (2019), 1231-1241 https://doi.org/10.1039/C9MD00044E
  2. Ali Hassoun, Peter K. Linden and Bruce Friedman, Incidence, prevalence, and management of MRSA bacteremia across patient populations—a review of recent developments in MRSA management and treatment, Critical Care, 21, 1, (2017), 211 https://doi.org/10.1186/s13054-017-1801-3
  3. C. J. Chen and Y. C. Huang, New epidemiology of Staphylococcus aureus infection in Asia, Clinical Microbiology and Infection, 20, 7, (2014), 605-623 https://doi.org/10.1111/1469-0691.12705
  4. Daniel J. Diekema, Michael A. Pfaller, Dee Shortridge, Marcus Zervos and Ronald N. Jones, Twenty-Year Trends in Antimicrobial Susceptibilities Among Staphylococcus aureus From the SENTRY Antimicrobial Surveillance Program, Open Forum Infectious Diseases, 6, Supplement_1, (2019), S47-S53 https://doi.org/10.1093/ofid/ofy270
  5. Olga Genilloud, Actinomycetes: still a source of novel antibiotics, Natural Product Reports, 34, 10, (2017), 1203-1232 https://doi.org/10.1039/C7NP00026J
  6. Binod Lekhak, Anjana Singh and Dwij Raj Bhatta, Antibacterial and Antifungal Property of Actinomycetes Isolates from Soil and Water of Nepal, Journal of Nepal Health Research Council, 16, 2, (2018), 136-139 https://doi.org/10.3126/jnhrc.v16i2.20298
  7. A. Asnani, D. Ryandini and Suwandri, Screening of Marine Actinomycetes from Segara Anakan for Natural Pigment and Hydrolytic Activities, IOP Conference Series: Materials Science and Engineering, 107, (2016), 012056 https://doi.org/10.1088/1757-899x/107/1/012056
  8. Rajaretinam Rajesh Kannan, Appadurai Muthamil Iniyan and Samuel Gnana Prakash Vincent, Production of a compound against methicillin resistant Staphylococcus aureus (MRSA) from Streptomyces rubrolavendulae ICN3 & its evaluation in zebrafish embryos, Indian Journal of Medical Research, 139, 6, (2014), 913-920
  9. Dong-Bo Xu, Wan-Wan Ye, Ying Han, Zi-Xin Deng and Kui Hong, Natural Products from Mangrove Actinomycetes, Marine Drugs, 12, 5, (2014), https://doi.org/10.3390/md12052590
  10. Sathish Kumar SR and Kokati Venkata Bhaskara Rao, In-vitro antimicrobial activity of marine actinobacteria against multidrug resistance Staphylococcus aureus, Asian Pacific Journal of Tropical Biomedicine, 2, 10, (2012), 787-792 https://doi.org/10.1016/S2221-1691(12)60230-5
  11. Mounyr Balouiri, Moulay Sadiki and Saad Koraichi Ibnsouda, Methods for in vitro evaluating antimicrobial activity: A review, Journal of Pharmaceutical Analysis, 6, 2, (2016), 71-79 https://doi.org/10.1016/j.jpha.2015.11.005
  12. Jan Hudzicki, Kirby-Bauer disk diffusion susceptibility test protocol, in, American Society of Microbiology, 2009,
  13. Koichiro Tamura, Glen Stecher, Daniel Peterson, Alan Filipski and Sudhir Kumar, MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0, Molecular Biology and Evolution, 30, 12, (2013), 2725-2729 https://doi.org/10.1093/molbev/mst197
  14. Yuliana Retnowati, Sukarti Moeljopawiro, Tjut Sugandawaty Djohan and Endang Sutariningsih Soetarto, Antimicrobial activities of actinomycete isolates from rhizospheric soils in different mangrove forests of Torosiaje, Gorontalo, Indonesia, Biodiversitas Journal of Biological Diversity, 19, 6, (2018), 2196-2203 https://doi.org/10.13057/biodiv/d190627
  15. Miguel A. Ramírez-Elías, Ronald Ferrera-Cerrato, Alejandro Alarcón, Juan J. Almaraz, Gustavo Ramírez-Valverde, Luz E. de-Bashan, Fernando J. Esparza-García and Oscar García-Barradas, Identification of culturable microbial functional groups isolated from the rhizosphere of four species of mangroves and their biotechnological potential, Applied Soil Ecology, 82, (2014), 1-10 https://doi.org/10.1016/j.apsoil.2014.05.001
  16. Arasu M. Valan, K. R. T. Asha, V. Duraipandiyan, S. Ignacimuthu and P. Agastian, Characterization and phylogenetic analysis of novel polyene type antimicrobial metabolite producing actinomycetes from marine sediments: Bay of Bengal India, Asian Pacific Journal of Tropical Biomedicine, 2, 10, (2012), 803-810
  17. https://doi.org/10.1016/S2221-1691(12)60233-0
  18. Suthinee Sangkanu, Vatcharin Rukachaisirikul, Chanwit Suriyachadkun and Souwalak Phongpaichit, Evaluation of antibacterial potential of mangrove sediment-derived actinomycetes, Microbial Pathogenesis, 112, (2017), 303-312 https://doi.org/10.1016/j.micpath.2017.10.010
  19. Naif Abdullah Al-Dhabi, Abdul-Kareem Mohammed Ghilan, Galal Ali Esmail, Mariadhas Valan Arasu, Veeramuthu Duraipandiyan and Karuppiah Ponmurugan, Bioactivity assessment of the Saudi Arabian Marine Streptomyces sp. Al-Dhabi-90, metabolic profiling and its in vitro inhibitory property against multidrug resistant and extended-spectrum beta-lactamase clinical bacterial pathogens, Journal of Infection and Public Health, 12, 4, (2019), 549-556 https://doi.org/10.1016/j.jiph.2019.01.065
  20. Hui Tian, Jamil Shafi, Mingshan Ji, Yuhui Bi and Zhiguo Yu, Antimicrobial Metabolites from Streptomyces sp. SN0280, Journal of Natural Products, 80, 4, (2017), 1015-1019 https://doi.org/10.1021/acs.jnatprod.6b01016
  21. Manish Sharma and Rajesh Kumari Manhas, Purification and characterization of actinomycins from Streptomyces strain M7 active against methicillin resistant Staphylococcus aureus and vancomycin resistant Enterococcus, BMC Microbiology, 19, 1, (2019), 44 https://doi.org/10.1186/s12866-019-1405-y
  22. Femina Wahaab and Kalidass Subramaniam, Bioprospecting marine actinomycetes for multidrug-resistant pathogen control from Rameswaram coastal area, Tamil Nadu, India, Archives of Microbiology, 200, 1, (2018), 57-71 https://doi.org/10.1007/s00203-017-1417-7
  23. Michael Goodfellow, Peter Kämpfer, Hans-Jürgen Busse, Martha E. Trujillo, Ken-ichiro Suzuki, Wolfgang Ludwig and William B. Whitman, Bergey’s Manual® of Systematic Bacteriology, Springer, New York, New York, 2012 https://doi.org/10.1007/978-0-387-68233-4

Last update:

  1. Marine Ascomycetes Extract Antifungal Susceptibility against Candida spp. Isolates from Oral Candidiasis HIV/AIDS Patient: An In Vitro Study

    Alexander Patera Nugraha, Mada Triandala Sibero, Kindi Farabi, Meircurius Dwi Condro Surboyo, Diah Savitri Ernawati, Tengku Natasha Eleena binti Tengku Ahmad Noor. European Journal of Dentistry, 18 (02), 2024. doi: 10.1055/s-0043-1768466

  2. Screening Anti-MRSA Activities of Indigenous Microbes and Prediction of The Biosynthetic Gene Clusters

    A Asnani, R Amaliyah, A Yuniaty. Journal of Physics: Conference Series, 1665 (1), 2020. doi: 10.1088/1742-6596/1665/1/012001

  3. Screening of Extracellular Enzyme from Phaeophyceae-Associated Fungi

    E P Ayuningtyas, M T Sibero, N E Br Hutapea, E H Frederick, R Murwani, D S Zilda, D P Wijayanti, A Sabdono, D Pringgenies, O K Radjasa. IOP Conference Series: Earth and Environmental Science, 750 (1), 2021. doi: 10.1088/1755-1315/750/1/012005

  4. The Production of Streptomyces W-5B Extract for Antibiofilm against Methicillin-resistant Staphylococcus aureus

    Ari Asnani, Azizah Purwanti, Windy Amalia Bakrudin, Dwi Utami Anjarwati. Journal of Pure and Applied Microbiology, 16 (1), 2022. doi: 10.22207/JPAM.16.1.23

  5. Utilization of winged bean (Psophocarpus tetragonolobus) seed powder as a coagulant and activated natural zeolite as an adsorbent for improving of tapioca and tofu wastewater qualities

    S R Istiqomah, S Hastuti, V Suryanti. Journal of Physics: Conference Series, 2556 (1), 2023. doi: 10.1088/1742-6596/2556/1/012001

  6. Interaction of mutant PBP2a and bioactive compounds from Streptomyces with anti-MRSA activities

    A Laksono, A Asnani, P Iswanto. IOP Conference Series: Materials Science and Engineering, 959 (1), 2020. doi: 10.1088/1757-899X/959/1/012031

Last update: 2024-11-19 03:01:24

  1. Screening Anti-MRSA Activities of Indigenous Microbes and Prediction of The Biosynthetic Gene Clusters

    A Asnani, R Amaliyah, A Yuniaty. Journal of Physics: Conference Series, 1665 (1), 2020. doi: 10.1088/1742-6596/1665/1/012001