Isolasi, Identifikasi serta Uji Aktivitas Sitotoksik Senyawa Alkaloid Total Daun Ketapang (Terminalia Catappa Linn.) dengan Metode Brine Shrimp Lethality Test (BSLT)

Choirulina Maulida; Dewi Kusrini; Enny Fachriyah

doi:10.14710/jksa.15.1.13-17

DOI: https://doi.org/10.14710/jksa.15.1.13-17

Isolasi, Identifikasi serta Uji Aktivitas Sitotoksik Senyawa Alkaloid Total Daun Ketapang (Terminalia Catappa Linn.) dengan Metode Brine Shrimp Lethality Test (BSLT)

Choirulina Maulida, Dewi Kusrini

, Enny Fachriyah

Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University, Indonesia

Published: 1 Apr 2012.

BibTex Citation Data :

@article{JKSA17589,
    author = {Choirulina Maulida and Dewi Kusrini and Enny Fachriyah},
    title = {Isolasi, Identifikasi serta Uji Aktivitas Sitotoksik Senyawa Alkaloid Total Daun Ketapang (Terminalia Catappa Linn.) dengan Metode Brine Shrimp Lethality Test (BSLT)},
    journal = {Jurnal Kimia Sains dan Aplikasi},
  volume = {15},
    number = {1},
    year = {2012},
    keywords = {Terminalia catappa Lin.; alkaloid; uji aktivitas; BSLT},
    abstract = {Isolasi senyawa alkaloid pada daun  T. catappa  yang merupakan tumbuhan famili combretaceae telah dilakukan. Isolasi alkaloid dilakukan dengan metode maserasi menggunakan pelarut n-heksana dilanjutkan dengan etanol. Ekstrak etanol diidentifikasi menggunakan pereaksi dragendorf dan meyer, diikuti dengan hidrolisis ekstrak etanol. Kemudian dilakukan ekstraksi cair-cair menggunakan pelarut etil asetat dan menghasilkan isolat alkaloid total. Analisis isolat alkaloid dilakukan dengan metode KLT dan pemisahan dengan KLT preparatif. Lalu, dilanjutkan dengan analisis menggunakan UV-Vis, FTIR dan LC-MS serta uji sitotoksik dengan metode BSLT. Isolat alkaloid dari daun  T. catappa  berupa serbuk berwarna putih. Analisis spektrofotometer UV-Vis menunjukkan bahwa isolat A1.1 dan A2.1 mempunyai λmax sebesar 220 nm. Sedangkan spektra FTIR menunjukkan bahwa masing-masing isolat memiliki bilangan gelombang 3441,01; 3425,58 cm -1  (vibrasi ulur O-H), 2924,09 dan 2854,65 cm -1  (vibrasi ulur C-H asimetri dan simetri), 1627,92 cm -1  (vibrasi ulur C=O), 1103,28 cm -1  (vibrasi ulur C-O yang simetri dengan vibrasi ulur C-N). Hasil LC-MS menunjukkan senyawa mempunyai berat molekul sebesar 337,34 g/mol (A1.1) dan 337,33 g/mol (A2.1). Senyawa tersebut diduga merupakan senyawa alkaloid tropane dengan gugus α, β asam tak jenuh dan isomernya. Uji sitotoksik menggunakan metode BSLT menunjukkan bahwa alkaloid total mempunyai harga LC 50  sebesar 132,590 ppm yang berarti alkaloid total bersifat tidak toksik.},
   issn = {2597-9914},   pages = {13--17}  doi = {10.14710/jksa.15.1.13-17},
    url = {https://ejournal.undip.ac.id/index.php/ksa/article/view/17589}
}

Citation Format:

Abstract

Isolasi senyawa alkaloid pada daun T. catappa yang merupakan tumbuhan famili combretaceae telah dilakukan. Isolasi alkaloid dilakukan dengan metode maserasi menggunakan pelarut n-heksana dilanjutkan dengan etanol. Ekstrak etanol diidentifikasi menggunakan pereaksi dragendorf dan meyer, diikuti dengan hidrolisis ekstrak etanol. Kemudian dilakukan ekstraksi cair-cair menggunakan pelarut etil asetat dan menghasilkan isolat alkaloid total. Analisis isolat alkaloid dilakukan dengan metode KLT dan pemisahan dengan KLT preparatif. Lalu, dilanjutkan dengan analisis menggunakan UV-Vis, FTIR dan LC-MS serta uji sitotoksik dengan metode BSLT. Isolat alkaloid dari daun T. catappa berupa serbuk berwarna putih. Analisis spektrofotometer UV-Vis menunjukkan bahwa isolat A1.1 dan A2.1 mempunyai λmax sebesar 220 nm. Sedangkan spektra FTIR menunjukkan bahwa masing-masing isolat memiliki bilangan gelombang 3441,01; 3425,58 cm^-1 (vibrasi ulur O-H), 2924,09 dan 2854,65 cm^-1 (vibrasi ulur C-H asimetri dan simetri), 1627,92 cm^-1 (vibrasi ulur C=O), 1103,28 cm^-1 (vibrasi ulur C-O yang simetri dengan vibrasi ulur C-N). Hasil LC-MS menunjukkan senyawa mempunyai berat molekul sebesar 337,34 g/mol (A1.1) dan 337,33 g/mol (A2.1). Senyawa tersebut diduga merupakan senyawa alkaloid tropane dengan gugus α, β asam tak jenuh dan isomernya. Uji sitotoksik menggunakan metode BSLT menunjukkan bahwa alkaloid total mempunyai harga LC₅₀ sebesar 132,590 ppm yang berarti alkaloid total bersifat tidak toksik.

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Keywords: Terminalia catappa Lin.; alkaloid; uji aktivitas; BSLT

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Section: Research Articles

Language : ID

In Vol 15, No 1 (2012): Volume 15 Issue 1 Year 2012

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