Analisis Kimiawi Fraksi n-Heksana dari Tanaman Purwoceng (Pimpinella Alpina Molk)

Zahroul Umami Qodri  -  Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University, Indonesia
*Bambang Cahyono scopus  -  Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University, Indonesia
Meiny Suzery orcid scopus  -  Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University, Indonesia
Published: 1 Apr 2013.
Open Access Copyright 2018 Jurnal Kimia Sains dan Aplikasi
License URL: http://creativecommons.org/licenses/by-sa/4.0

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Article Info
Section: Research Articles
Language: ID
Statistics: 464 6513
Abstract
Telah dilakukan kuantifikasi kandungan fenolat total, flavonoid total, uji aktivitas antioksidan (DPPH), serta identifikasi secara kromatografi senyawa fenolat dari golongan kumarin dalam fraksi n-heksana Pimpinella alpina Molk. Hasil penelitian menunjukkan bahwa kandungan senyawa fenolat total fraksi n-heksana , yaitu 45,04 mg ekuivalen asam galat/gram fraksi kering. Sedangkan kandungan flavonoid total fraksi n-heksana yaitu 5,58 mg ekuivalen kuersetin/gram fraksi kering. Aktivitas antioksidan dengan metode DPPH diperoleh IC50 yaitu 13485 ppm. Identifikasi dari golongan kumarin dari kromatografi kolom dihasilkan 11 fraksi, berdasarkan hasil profil KLT setelah disemprot dengan pereaksi warna NH3 dan dilihat di bawah lampu UV 365 nm, ditunjukkan bahwa fraksi F1 positif mengandung golongan senyawa kumarin yang ditunjukkan oleh noda berwarna hijau kuning. Bagaimanapun struktur kimia dari senyawa tersebut hingga saat ini belum dapat diusulkan.
Keywords: Pimpinella alpina; antioksidan; kumarin

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