Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University, Indonesia
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@article{JKSA18461, author = {Yuga Pratama and Purbowatiningrum Sarjono and Nies Mulyani}, title = {Skrining Metabolit Sekunder Bakteri Endofit yang Berfungsi sebagai Antidiabetes dari Daun Mimba (Azadirachta Indica)}, journal = {Jurnal Kimia Sains dan Aplikasi}, volume = {18}, number = {2}, year = {2015}, keywords = {Daun mimba; bakteri endofit; metabolit sekunder; enzim α-glukosidase}, abstract = {Telah dilakukan penelitian tentang skrining metabolit sekunder bakteri endofit yang berfungsi sebagai antidiabetes dari daun mimba ( Azadirachta indica ). Penelitian ini bertujuan memperoleh data morfologi, memperoleh data kurva pertumbuhan isolat bakteri endofit dari daun mimba, memperoleh hasil inhibisi metabolit sekunder bakteri endofit dari daun miba terhadap α-glukosidase, serta mendapatkan informasi mengenai kandungan kimia dari metabolit sekunder isolat bakteri endofit yang didapat. Aktivitas antidiabetes diuji menggunakan metode penghambatan enzim α-glukosidase. Hasil yang diperoleh adalah bakteri endofit A5 dan A6 dari daun mimba merupakan bakteri dengan jenis bakteri gram negatif, dengan bentuk penampakan mikroskopis yang berbeda. Berdasarkan data kurva pertumbuhan bakteri endofit dapat disimpulkan bahwa untuk memperoleh metabolit sekunder adalah pada jam ke-33 hingga jam ke-48 untuk isolat A5 dan jam ke-36 hingga jam ke-48 untuk isolat A6. Adapun daya inhibisi aktivitas α-glukosidase optimal dari metabolit sekunder A5 dan A6 masing-masing pada konsentrasi 62,5 ppm berturut-turut sebesar 18,462% dan 20,173%. Penapisan fitokimia pada penelitian ini masing-masing metabolit sekunder bakteri endofit A5 dan A6 dari daun mimba mengandung senyawa flavonoid, saponin, dan terpenoid/steroid.}, issn = {2597-9914}, pages = {73--78} doi = {10.14710/jksa.18.2.73-78}, url = {https://ejournal.undip.ac.id/index.php/ksa/article/view/18461} }
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