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Potensi Senyawa Antibakteri dari Ekstrak Akar Manis (Glycyrrhiza glabra L) terhadap Bacillus cereus

Potential of Antibacterial Compounds from Sweet Root Extract (Glycyrrhiza glabra L) on Bacillus cereus

Universitas 17 Agustus 1945, Indonesia

Received: 18 Feb 2019; Revised: 7 May 2019; Accepted: 13 May 2019; Available online: 31 May 2019; Published: 31 Jul 2019.
Open Access Copyright 2019 Jurnal Kimia Sains dan Aplikasi under http://creativecommons.org/licenses/by-sa/4.0.

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Abstract

The high number of poisoning food and diarrheal diseases caused by Bacillus cereus bacteria has been treated with chemical drugs and traditionally herbal plants. One of the commonly used herbal plants is sweet root (Glycyrrhiza glabra L.). This study aims to extract and fractionate sweet root plants, determine the antibacterial activity and identify the types of bioactive compounds as antibacterial compounds. The extraction process uses a maceration technique and fractionation using buthanol, ethyl acetate, and hexan solvents. Antibacterial activity was carry out by the diffusion method and identification of bioactive compounds by GCMS analysis. This study showed that greatest antibacterial activity was found in ethyl acetate fraction with a strong category and MIC value of 12,5%. This study for the first time also revealed that the types of bioactive compounds from sweet root plants (Glycyrrhiza glabra L.) as antibacterial compounds are n-Hexadecanoic as a fatty acid group and 4H-Pyran 4-one 2.3 dihydro-3, 5-dihydroxy-6 methyl from the flavonoid group.

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Keywords: Antibacterial; Bacillus cereus; bioactive; Glycyrrhiza glabra L; sweet root
Funding: Faculty of Pharmacy, 17 Augustus 1945 University, Jakarta 14350, Indonesia

Article Metrics:

  1. Badan Pengawas Obat dan Makanan, Kriteria dan tata laksana pendaftaran obat tradisional, obat herbal terstandar dan fitofarmaka, in: B.P.O.d. Makanan (Ed.) HK.00.05.41.13, Jakarta, 2005
  2. Siti Zulaekah, Pendidikan Gizi Dengan Media Booklet Terhadap Pengetahuan Gizi, KEMAS: Jurnal Kesehatan Masyarakat, 7, 2, (2012) 127-133 10.15294/kemas.v7i2.2808
  3. Harold Richardson, Fiona Smaill, Medical microbiology, BMJ, 317, 7165, (1998) 1060 https://doi.org/10.1136/bmj.317.7165.1060
  4. Rullah Hermanda, Wahyu Widayat, Laode Rijai, Aktivitas Antibakteri Ekstrak Metanol Akar Tumbuhan Merung (Coptosapelta tomentosa) terhadap Bakteri Escherichia Coli dan Staphylococcus aureus, in: Mulawarman Pharmaceuticals Conferences, 2016, pp. 322-329
  5. Sulistiorini Indriaty, Lela Sulastri, Uji Aktivitas Ekstrak Air Akar Manis (Glycyrrhiza glabra L.) Sebagai Penyubur Rambut Pada Kelinci Jantan, Medical Sains; Jurnal Ilmiah Kefarmasian, 1, 1, (2016) http://dx.doi.org/10.1234/ms.v1i1.11
  6. Dinesh Dhingra, Amandeep Sharma, Antidepressant-like activity of Glycyrrhiza glabra L. in mouse models of immobility tests, Prog Neuropsychopharmacol Biol Psychiatry, 30, 3, (2006) 449-454 https://doi.org/10.1016/j.pnpbp.2005.11.019
  7. Giulia Pastorino, Laura Cornara, Sónia Soares, Francisca Rodrigues, M. Beatriz P.P. Oliveira, Liquorice (Glycyrrhiza glabra): A phytochemical and pharmacological review, Phytotherapy Research, 32, 12, (2018) 2323-2339 https://doi.org/10.1002/ptr.6178
  8. Faruk Karahan, Cumhur Avsar, Ibrahim Ilker Ozyigit, Ismet Berber, Antimicrobial and antioxidant activities of medicinal plant Glycyrrhiza glabra var. glandulifera from different habitats, Biotechnology & Biotechnological Equipment, 30, 4, (2016) 797-804 https://doi.org/10.1080/13102818.2016.1179590
  9. Departemen Kesehatan RI, Parameter standar umum ekstrak tumbuhan obat, Direktorat Jenderal Pengawasan Obat dan Makanan, Jakarta, 2000
  10. Anjar Purba Asmara, Uji Fitokimia Senyawa Metabolit Sekunder Dalam Ekstrak Metanol Bunga Turi Merah (Sesbania grandiflora L. Pers), Al-Kimia, 5, 1, (2017) 48-59 https://doi.org/10.24252/al-kimia.v5i1.2856
  11. Moch. Chasani, Ruli Budi Fitriaji, Purwati, Fraksinasi Ekstrak Metanol Kulit Batang Ketapang (Terminalia catappa Linn.) dan Uji Toksisitasnya dengan Metode BSLT (Brine Shrimp Lethality Test), Molekul, 8, 1, (2013) 89-100
  12. Himanshu Aggarwal, Jayashri Ghosh, Alka Rao, Vinod Chhokar, Evaluation of Root and Leaf Extracts of Glycrriza Glabra for Antimicrobial Activity, Journal of Medical and Bioengineering, 4, 1, (2015) 81-85 http://doi.org/10.12720/jomb.4.1.81-85
  13. Norman R. Farnsworth, Biological and Phytochemical Screening of Plants, Journal of Pharmaceutical Sciences, 55, 3, (1966) 225-276 https://doi.org/10.1002/jps.2600550302
  14. Sogandi, Apon Zaenal Mustopa, I Made Artika, Bugi Ratno Budiarto, Inhibitory Activity of Lactobacillus plantarum U10 Isolated from Tempoyak (Fermented Durian) Made in Indonesia against Salmonella typhi, Microbiology Indonesia, 9, 2, (2015) 73-81 http://doi.org/10.5454/mi.9.2.4
  15. Sogandi, Frensiska Anggelia, Lilih Riniwasih K, Uji Aktivitas Antibakteri Ekstrak Etanol 96% Daun Rambai (Sonneratia caseolaris, (L.) Engl) terhadap Pertumbuhan Bakteri Escherichia col, Indonesia Natural Research Pharmaceutical Journal, 2, 1, (2017) 73-80
  16. Risa Nursanty, Aktivitas antibakteri ekstrak metanol tanaman Lawsonia inermis L. dan Capsicum frutescens L. terhadap bakteri Staphylococcus aureus ATCC 29213, Bioleuser, 1, 3, (2017) 98-103
  17. Rita Dwi Ratnani, Indah Hartati, Yance Anas, Devi Endah P., Dita Desti D. Khilyati, Standardisasi Spesifik dan Non Spesifik Ekstraksi Hidrotropi Andrographolid dari Sambiloto (Andrographis paniculata), Seminar Nasional Peluang Herbal Sebagai Alternatif Medicine Tahun 2015, Semarang, (2015)
  18. Irvina Nurrachmi, Bintal Amin, Dessy Yoswaty, Analisis Senyawa Bioaktif Ekstrak Heksan, Etil Asetat dan Metanol Rumput Laut (Eucheuma cottonii) dari Pantai Pulau Jaga, Karimun Provinsi Kepulauan Riau, Asian Journal of Environment, History and Heritage, 2, 1, (2018) 105-112
  19. Divya Gupta, Mukesh Kumar, Evaluation of in vitro antimicrobial potential and GC–MS analysis of Camellia sinensis and Terminalia arjuna, Biotechnology Reports, 13, (2017) 19-25 https://doi.org/10.1016/j.btre.2016.11.002
  20. Gebremedhin Romha, Birhanu Admasu, Tsegaye Hiwot Gebrekidan, Hailelule Aleme, Gebreyohans Gebru, Antibacterial Activities of Five Medicinal Plants in Ethiopia against Some Human and Animal Pathogens, Evidence-Based Complementary and Alternative Medicine, 2018, (2018) 10 https://doi.org/10.1155/2018/2950758
  21. Imad Hadi Hameed, Hussein J. Hussein, Muhanned Abdulhasan Kareem, Nidaa Shihab Hamad, Identification of five newly described bioactive chemical compounds in methanolic extract of Mentha viridis by using gas chromatography - mass spectrometry (GC-MS), Journal of Pharmacognosy and Phytotherapy, 7, 7, (2015) 107-125 https://doi.org/10.5897/JPP2015.0349
  22. W. W. Davis, T. R. Stout, Disc plate method of microbiological antibiotic assay. II. Novel Procedure Offering Improved Accuracy, Applied microbiology, 22, 4, (1971) 666–670
  23. S Rodino, A. Butu, M. Butu, P.C. Cornea, Comparative Studies on Antibacterial Activity of Licorice, Elderberry and Dandelion, Digest Journal of Nanomaterials and Biostructures, 10, 3, (2015) 947-955
  24. K. Radha Krishnan, F. James, A. Mohan, Isolation and characterization of n-hexadecanoic acid from Canthium parviflorum leaves, Journal of Chemical and Pharmaceutical Research, 8, 8, (2016) 614-617
  25. Zhong-hui Pu, Yu-qun Zhang, Zhong-qiong Yin, Jiao Xu, Ren-yong Jia, Yang Lu, Fan Yang, Antibacterial Activity of 9-Octadecanoic Acid-Hexadecanoic Acid-Tetrahydrofuran-3,4-Diyl Ester from Neem Oil, Agricultural Sciences in China, 9, 8, (2010) 1236-1240 https://doi.org/10.1016/S1671-2927(09)60212-1
  26. Aurelio López-Malo, Stella Maris Alzamora, Enrique Palou, Aspergillus flavus growth in the presence of chemical preservatives and naturally occurring antimicrobial compounds, International journal of food microbiology, 99, 2, (2005) 119-128 https://doi.org/10.1016/j.ijfoodmicro.2004.08.010
  27. Purnama Darmadji, Masatoshi Izumimoto, Effect of chitosan in meat preservation, Meat Science, 38, 2, (1994) 243-254 https://doi.org/10.1016/0309-1740(94)90114-7
  28. Juliana Bodoprost, Helmut Rosemeyer, Analysis of Phenacylester Derivatives of Fatty Acids from Human Skin Surface Sebum by Reversed-Phase HPLC: Chromatographic Mobility as a Function of Physico-Chemical Properties, International Journal of Molecular Sciences, 8, 11, (2007) 1111-1124
  29. Rengasamy Ragupathi Raja Kannan, Radjassegarin Arumugam, Perumal Anantharaman, Chemical composition and antibacterial activity of Indian seagrasses against urinary tract pathogens, Food Chemistry, 135, 4, (2012) 2470-2473 https://doi.org/10.1016/j.foodchem.2012.07.070
  30. P. Awa, S. Ibrahim, D. A. Ameh, GC/MS Analysis and Antimicrobial Activity of Diethyl Ether Fraction of Methanolic Extract from the stem bark of Annona senegalensis Pers., International Journal of Pharmaceutical Sciences and Research, 3, 11, (2012) 4213-4218 http://dx.doi.org/10.13040/IJPSR.0975-8232.3(11).4213-18
  31. Filomena Conforti, Mariangela Marrelli, Giancarlo Statti, Federica Menichini, Dimitar Uzunov, Umberto Solimene, Francesco Menichini, Comparative chemical composition and antioxidant activity of Calamintha nepeta (L.) Savi subsp. glandulosa (Req.) Nyman and Calamintha grandiflora (L.) Moench (Labiatae), Natural Product Research, 26, 1, (2012) 91-97 10.1080/14786419.2010.545356
  32. Kavitha Alli, Lakshmi Narasu Mangamoori, Phytochemical compound identification and evaluation of antimicrobial activity of Eugenia Bracteata Roxb, International Journal of Biotechnology and Biochemistry, 12, 1, (2016) 73-83

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