skip to main content

Aktivitas Antibakteri Loloh (Obat Tradisional Bali) Air Perasan dan Air Rebusan Daun Sirih terhadap Bakteri Streptococcus pyogenes Penyebab Radang Tenggorokan

Antibacterial Activity of Loloh (Traditional Balinese Medicine) Decoction Water and Squeeze Water of betel Leaf against Bacteria Streptococcus pyogenes causes of sore throat

Fakultas Teknologi Informasi dan Sains, Universitas Hindu Indonesia, Indonesia

Received: 29 Apr 2019; Revised: 2 Aug 2019; Accepted: 9 Aug 2019; Published: 30 Sep 2019.
Open Access Copyright 2019 Jurnal Kimia Sains dan Aplikasi under http://creativecommons.org/licenses/by-sa/4.0.

Citation Format:
Cover Image
Abstract
Sore throat is a respiratory infection caused by a bacterial infection of Streptococcus pyogenes. Betel leaf is used for loloh (traditional medicine) which has antimicrobial activity. The purpose of this study was to determine the content of active compounds and the antibacterial activity of the boiled water and juice of betel leaf against Streptococcus pyogenes. Explorative research using GC-MS method was used to identify and determine the abundance (content) of active compounds from loloh while the experimental research with the Randomized Post Test Only Control Group Design was carried out by treating the samples in four groups those are the negative control group with distilled water, the positive control group with 30 μg vancomycin, the treatment group with boiled water of betel leaf and betel leaf juice water to prove the antibacterial activity of loloh. The antibacterial activity data of the study results were analyzed statistically nonparametric through the Kruskal-Wallis test. The results showed that the betel leaf boiled water contained 0.472% hydroxicavicol active compound and had an average antibacterial activity of 6.50±0.224 mm against Streptococcus pyogenes bacteria. Whilst the juice of betel leaves did not contain active compounds and had no antibacterial activity.

Note: This article has supplementary file(s).

Fulltext View|Download |  Transcripts
Untitled
Subject
Type Transcripts
  Download (2MB)    Indexing metadata
Keywords: Betel leaf; Loloh; antibacterial; Streptococcus pyogenes
Funding: Universitas Hindu Indonesia

Article Metrics:

  1. World Health Organization. The top 10 causes of death. 2018; Available from: http://www.who.int/news-room/fact-sheets/detail/the-top-10-causes-of-death
  2. Mayo Clinic. Strep Throat. 2018; Available from: https://www.mayoclinic.org/diseases-conditions/strep-throat/symptoms-causes/syc-20350338
  3. Sri Agung Fitri Kusuma, Bakteri Streptococcus pyogenes, in: U. Padjadjaran (Ed.), Bandung, 2010
  4. Wawan Sujarwo, Ary Prihardhyanto Keim, Valentina Savo, Paolo Maria Guarrera, Giulia Caneva, Ethnobotanical study of Loloh: Traditional herbal drinks from Bali (Indonesia), Journal of Ethnopharmacology, 169, (2015) 34-48 https://doi.org/10.1016/j.jep.2015.03.079
  5. L.S.R Arambewela, L.D.A.M Arawwawala, K Kumaratunga, D Dissanayake, W Ratnasooriya, S Kumarasingha, Investigations on Piper betle grown in Sri Lanka, Pharmacognosy Reviews, 5, 10, (2011) 159-163 http://doi.org/10.4103/0973-7847.91111
  6. T Nalina, ZHA Rahim, The crude aqueous extract of Piper betle L. and its antibacterial effect towards Streptococcus mutans, American Journal of Biochemistry and Biotechnology, 3, 1, (2007) 10-15 http://doi.org/10.3844/ajbbsp.2007.10.15
  7. KY Pin, A Luqman Chuah, A Abdull Rashih, MP Mazura, J Fadzureena, S Vimala, MA Rasadah, Antioxidant and Anti-Inflammatory Activities of Extracts of Betel Leaves (Piper Betle) from Solvents with Different Polarities, Journal of Tropical Forest Science, 22, 4, (2010) 448-455
  8. Ataç Uzel, Kadri˙ye Sorkun, Özant Önçağ, Dilşah Çoğulu, Ömür Gençay, Beki˙r Sali˙h, Chemical compositions and antimicrobial activities of four different Anatolian propolis samples, Microbiological Research, 160, 2, (2005) 189-195 https://doi.org/10.1016/j.micres.2005.01.002
  9. Kusriningrum Rochiman, Dasar Perancangan Percobaan dan Rancangan Acak Lengkap, Universitas Airlangga, 1989
  10. A Syahidah, CR Saad, MD Hassan, Y Rukayadi, MH Norazian, MS Kamarudin, Phytochemical analysis, identification and quantification of antibacterial active compounds in betel leaves, Piper betle methanolic extract, Pakistan Journal of Biological Sciences, 20, 2, (2017) 70-81 http://doi.org/10.3923/pjbs.2017.70.81
  11. I. Thamaraikani, M. Kulandhaivel, Purification of Hydroxychavicol from Piper betle Linn and Evaluation of Antimicrobial Activity against Some Food Poison Causing Bacteria, Journal of Pure and Applied Microbiology, 11, (2017) 1883-1889 http://doi.org/10.22207/JPAM.11.4.28
  12. Eisuke Kato, Ryo Nakagomi, Maria D. P. T. Gunawan-Puteri, Jun Kawabata, Identification of hydroxychavicol and its dimers, the lipase inhibitors contained in the Indonesian spice, Eugenia polyantha, Food Chemistry, 136, 3, (2013) 1239-1242 https://doi.org/10.1016/j.foodchem.2012.09.013
  13. Eric E. Conn, Outlines of biochemistry, Wiley, 1987
  14. M. C. Chang, B. J. Uang, C. Y. Tsai, H. L. Wu, B. R. Lin, C. S. Lee, Y. J. Chen, C. H. Chang, Y. L. Tsai, C. J. Kao, J. H. Jeng, Hydroxychavicol, a novel betel leaf component, inhibits platelet aggregation by suppression of cyclooxygenase, thromboxane production and calcium mobilization, British Journal of Pharmacology, 152, 1, (2007) 73-82 http://doi.org/10.1038/sj.bjp.0707367
  15. Intzar Ali, Farrah G. Khan, Krishan A. Suri, Bishan D. Gupta, Naresh K. Satti, Prabhu Dutt, Farhat Afrin, Ghulam N. Qazi, Inshad A. Khan, In vitro antifungal activity of hydroxychavicol isolated from Piper betle L, Annals of Clinical Microbiology and Antimicrobials, 9, 1, (2010) 7 http://doi.org/10.1186/1476-0711-9-7
  16. Chayanika Sarma, Prasad Rasane, Sawinder Kaur, Jyoti Singh, Joginder Singh, Yogesh Gat, Umar Garba, Damanpreet Kaur, Kajal Dhawan, Antioxidant and antimicrobial potential of selected varieties of Piper betle L. (Betel leaf), Anais da Academia Brasileira de Ciências, 90, 4, (2018) 3871-3878 https://dx.doi.org/10.1590/0001-3765201820180285
  17. Kaveti Balaji, Lisa tan, Sarnia, Sin kuan, Mirza Baig, Antibacterial Activity Of Piper Betel Leaves, International Journal of Pharmacy Teaching & Practices, 2, 3, (2011) 129-132
  18. Suliantari, Aktivitas Antibakteri dan Mekanisme Penghambatan Ekstrak Sirih Hijau (Piper betle Linn) terhadap Bakteri Patogen Pangan, Program Studi Ilmu Pangan, Institut Pertanian Bogor, Bogor
  19. Lu'lu A'lana, Rafika Sari, Pratiwi Apridamayanti, Penentuan Nilai FICI Kombinasi Ekstrak Etanol Kulit Daun Lidah Buaya (Aloe vera (L) Burm.f) dan Gentamisin Sulfat Terhadap Bakteri Escherichia coli, Pharmaceutical Sciences and Research (PSR), 4, 3, (2018) 132-142 http://dx.doi.org/10.7454/psr.v4i3.3695
  20. Christina Andhika Setyanti. Resep Jamu Daun Sirih Sederhana ala Mustika Ratu. 2015; Available from: https://www.cnnindonesia.com/gaya-hidup/2015042-2201134-262-48580/resep-jamu-daun-sirih-sederhana-ala-mustika-ratu

Last update:

No citation recorded.

Last update: 2024-03-28 14:36:11

No citation recorded.