DOI: https://doi.org/10.14710/jksa.23.10.353-359

Comparative Optimization of Cellulase and Laccase Enzymes in Deinking Process of Used Newspapers

1Cellulosic and Functional Materials Research Centre (CFM-RC), Universitas Sumatera Utara, Indonesia

2Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Sumatera Utara, Indonesia

Received: 23 Apr 2020; Revised: 26 Sep 2020; Accepted: 8 Oct 2020; Published: 31 Oct 2020.
Open Access Copyright 2020 Jurnal Kimia Sains dan Aplikasi under http://creativecommons.org/licenses/by-sa/4.0.

Citation Format:
Cover Image
Abstract

The use of enzymes in the bio-deinking process of newspaper waste has promising potential. However, investigations on the concentration of enzyme combinations need to be carried out to obtain the optimum ratio of cellulase and laccase enzymes for the bio-deinking process of recycled newspapers. The mixture of the two enzymes at various ratios was used to remove the ink on paper pulp from used newspapers by mechanical disintegration method treatment and followed by the bio-deinking process in an incubator shaker. The characterization of functional groups, structures, and thermal properties of bio-deinked pulp paper was carried out by FTIR, XRD, DTG/TGA, and an analysis of the degree of brightness to the prepared paper. FTIR results confirmed three main components of papers, such as cellulose, hemicellulose, and lignin. The XRD results showed that the equal ratio of cellulase and laccase enzymes had an effect on a higher crystallinity index, which was 78.8% compared to those obtained from the conventional methods with a crystallinity index of 69.7%. Thermal analysis showed that the optimum combination of both enzymes contributed the most at the highest temperature where the rate of degradation decreased. Brightness analysis showed that bio-deinking had met the quality requirements for newsprint paper in SNI 7273:2008. Our findings show that the combination of cellulase and laccase enzymes at the same ratio can produce optimal bio-deinked pulp for paper fabrication with excellent characteristics in brightness, thermal, and physical properties.

Fulltext View|Download
Keywords: newspaper bio-deinking; cellulase enzymes; laccase enzymes
Funding: Universitas Sumatera Utara

Article Metrics:

Article Info
Section: Research Articles
Language : EN
Recent articles
Study of Kinetics and Adsorption Isotherm of Methylene Blue Dye using Tannin Gel from Ceriops tagal Synthesis and Characterization of SiO2/ZnO Nanocomposites from Zinc Waste and Mount Merapi Volcanic Ash Reinforcement of Quaternary Ammonium Modified Silica (QAMS) with Magnetite and its Application by Solid Phase Adsorption (SPA) to Adsorb Chromate Ions More recent articles
Most cited articles
Studi Filogeni dan Uji Potensi Antibakteri serta Enzim Ekstraseluler Isolat Geobacillus sp. dari Sumber Air Panas Gedongsongo Interlayer-free Silica Pectin Membrane for Wetland Saline Water via Pervaporation Pengelolaan Alat Bahan dan Laboratorium Kimia Chitosan succinate/PVA-PEG Membrane: Preparation, Characterization and Permeation Ability Test on Creatinine Antioxidant Activity of Daemonorops draco Resin More cited articles
  1. Mohammadreza Kamali, Zahra Khodaparast, Review on recent developments on pulp and paper mill wastewater treatment, Ecotoxicology and Environmental Safety, 114, (2015), 326-342 https://doi.org/10.1016/j.ecoenv.2014.05.005
  2. Arunika Saxena, Prakram Singh Chauhan, Role of various enzymes for deinking paper: a review, Critical Reviews in Biotechnology, 37, 5, (2017), 598-612 https://doi.org/10.1080/07388551.2016.1207594
  3. S. Gea, N. Panindia, A. F. Piliang, A. Sembiring, Y. A. Hutapea, All-cellulose composite isolated from oil palm empty fruit bunch, Journal of Physics: Conference Series, 1116, (2018), 042013 http://dx.doi.org/10.1088/1742-6596/1116/4/042013
  4. S. Gea, Z. Zulfahmi, D. Yunus, A. Andriayani, Y. A. Hutapea, The Isolation of Nanofibre Cellulose from Oil Palm Empty Fruit Bunch Via Steam Explosion and Hydrolysis with HCl 10%, Journal of Physics: Conference Series, 979, (2018), 012063 http://dx.doi.org/10.1088/1742-6596/979/1/012063
  5. David Ibarra, M. Concepción Monte, Angeles Blanco, Angel T. Martínez, María J. Martínez, Enzymatic deinking of secondary fibers: cellulases/hemicellulases versus laccase-mediator system, Journal of Industrial Microbiology & Biotechnology, 39, 1, (2012), 1-9 https://doi.org/10.1007/s10295-011-0991-y
  6. Dhara I. Desai, Bragadish D. Iyer, Biodeinking of old newspaper pulp using a cellulase-free xylanase preparation of Aspergillus niger DX-23, Biocatalysis and Agricultural Biotechnology, 5, (2016), 78-85 https://doi.org/10.1016/j.bcab.2015.11.001
  7. Saharman Gea, Sri Rahayu, Andriayani Andriayani, Averroes Fazlur Rahman Piliang, Noni Oktari, Mechanical and morphological characteristic investigations of deinked used newsprint paper via ultra-sonochemistry method, Jurnal Natural, 20, 2, (2020), 49-55 https://doi.org/10.24815/jn.v20i2.16649
  8. Muryeti, Estuti Budi Mulyani, Elya Sinurat, Adsorption of Carbon Black Using Chitosan in the Deinking Process, Procedia Chemistry, 17, (2015), 106-110 https://doi.org/10.1016/j.proche.2015.12.117
  9. M. Zulham Efendi Sinaga, Saharman Gea, Nami Panindia, Yuan Alfinsyah Sihombing, The Preparation of All-Cellulose Nanocomposite Film from Isolated Cellulose of Corncobs as Food Packaging, Oriental Journal of Chemistry, 34, 1, (2018), 562 http://dx.doi.org/10.13005/ojc/340166
  10. Cristina Campano, Ruben Miranda, Noemi Merayo, Carlos Negro, Angeles Blanco, Direct production of cellulose nanocrystals from old newspapers and recycled newsprint, Carbohydrate Polymers, 173, (2017), 489-496 https://doi.org/10.1016/j.carbpol.2017.05.073
  11. Ander Orue, Arantzazu Santamaria-Echart, Arantxa Eceiza, Cristina Peña-Rodriguez, Aitor Arbelaiz, Office waste paper as cellulose nanocrystal source, Journal of Applied Polymer Science, 134, 35, (2017), 45257 https://doi.org/10.1002/app.45257
  12. Ana R. Prazeres, Silvana Luz, Flávia Fernandes, Eliana Jerónimo, Cheese wastewater treatment by acid and basic precipitation: Application of H2SO4, HNO3, HCl, Ca(OH)2 and NaOH, Journal of Environmental Chemical Engineering, 8, 2, (2020), 103556 https://doi.org/10.1016/j.jece.2019.103556
  13. Avtar Singh, Ravi Dutt Yadav, Amanjot Kaur, Ritu Mahajan, An ecofriendly cost effective enzymatic methodology for deinking of school waste paper, Bioresource Technology, 120, (2012), 322-327 https://doi.org/10.1016/j.biortech.2012.06.050
  14. S. K. Singh, Shilpa Kulkarni, Vivek Kumar, Prabhat Vashistha, Sustainable utilization of deinking paper mill sludge for the manufacture of building bricks, Journal of Cleaner Production, 204, (2018), 321-333 https://doi.org/10.1016/j.jclepro.2018.09.028
  15. Carlos Vila, Agustín García Barneto, Amanda Fillat, Teresa Vidal, José Ariza, Use of thermogravimetric analysis to monitor the effects of natural laccase mediators on flax pulp, Bioresource Technology, 102, 11, (2011), 6554-6561 https://doi.org/10.1016/j.biortech.2011.03.068
  16. Antar Puneet Virk, Minakshi Puri, Vijaya Gupta, Neena Capalash, Prince Sharma, Combined Enzymatic and Physical Deinking Methodology for Efficient Eco-Friendly Recycling of Old Newsprint, PLOS ONE, 8, 8, (2013), e72346 https://doi.org/10.1371/journal.pone.0072346
  17. Q. H. Xu, Y. P. Wang, M. H. Qin, Y. J. Fu, Z. Q. Li, F. S. Zhang, J. H. Li, Fiber surface characterization of old newsprint pulp deinked by combining hemicellulase with laccase-mediator system, Bioresource Technology, 102, 11, (2011), 6536-6540 https://doi.org/10.1016/j.biortech.2011.03.051
  18. Hader H. Alzate Gil, Andres Mauricio Dovale S., Virney Hadely Chala L., Oscar A. Muñoz, Ana Elisa Casas B., German Camilo Quintana M., Jorge A. Velasquez J., Study of the enzymatic/neutral deinking process of waste photocopy paper, O Papel, 74, (2013), 61-65
  19. D. E. Tsatsis, D. K. Papachristos, K. A. Valta, A. G. Vlyssides, D. G. Economides, Enzymatic deinking for recycling of office waste paper, Journal of Environmental Chemical Engineering, 5, 2, (2017), 1744-1753 https://doi.org/10.1016/j.jece.2017.03.007
  20. Fina Khaerunnisa Frima, Rina Budi Satiyarti, Yulistia Anggraini, Erga Syafitri, Ika Agus Rini, Characteristics of Raw-Starch Degrading Amylase Bacteria from Natar Hot Spring Lampung, Jurnal Kimia Sains dan Aplikasi, 23, 7, (2020), 238-253 https://doi.org/10.14710/jksa.23.7.238-243
  21. Shiv Shankar, Shikha, Chandra Bhan, Rajesh Chandra, Sanjay Tyagi, Laccase based de-inking of mixed office waste and evaluation of its impact on physico-optical properties of recycled fiber, Environmental Sustainability, 1, 3, (2018), 233-244 https://doi.org/10.1007/s42398-018-0021-3
  22. Pratima Bajpai, Recycling and Deinking of Recovered Paper, Elsevier, London, 2013
  23. Sunkyu Park, John O. Baker, Michael E. Himmel, Philip A. Parilla, David K. Johnson, Cellulose crystallinity index: measurement techniques and their impact on interpreting cellulase performance, Biotechnology for Biofuels, 3, 1, (2010), 10 https://doi.org/10.1186/1754-6834-3-10
  24. Mohammad Mazedul Kabir, Md Mainul Islam, Hao Wang, Mechanical and thermal properties of jute fibre reinforced composites, Journal of Multifunctional Composites, 1, 1, (2013), 71-77
  25. A. Awal, S. B. Ghosh, M. Sain, Thermal properties and spectral characterization of wood pulp reinforced bio-composite fibers, Journal of Thermal Analysis and Calorimetry, 99, 2, (2010), 695-701 https://doi.org/10.1007/s10973-009-0100-x
  26. Nadežda Števulova, Viola Hospodárova, Adriana Eštoková, Study of thermal analysis of selected cellulose fibres, GeoScience Engineering, 62, 3, (2016), 18-21 http://doi.org/10.1515/gse-2016-0020
  27. SF Ibrahim, ES El-Amoudy, KE Shady, Thermal analysis and characterization of some cellulosic fabrics dyed by a new natural dye and mordanted with different mordants, International Journal of Chemistry, 3, 2, (2011), 40-54 https://doi.org/10.5539/ijc.v3n2p40

Last update:

  1. Synergistic deinking effect of neutral sodium sulfite with fungal hemicellulase enzyme for improved recycling of waste papers

    Iman Akbarpour. International Journal of Biological Macromolecules, 282 , 2024. doi: 10.1016/j.ijbiomac.2024.137010

  2. Biological approach in deinking of waste paper using bacterial cellulase as an effective enzyme catalyst

    T. Indumathi, Rita Jayaraj, P. Senthil Kumar, Mary Isabella Sonali J, Veena Gayathri Krishnaswamy, Ayman A. Ghfar, Saravanan Govindaraju. Chemosphere, 287 , 2022. doi: 10.1016/j.chemosphere.2021.132088

  3. Ultrasound pretreatment and enzymatic deinking by using bacterial cellulases from rice husk

    Saharman Gea, Rumondang Bulan, Emma Zaidar, Averroes Fazlurrahman Piliang, Noni Oktari, Sri Rahayu, Yasir Arafat Hutapea, Reka Mustika Sari. Journal of Wood Chemistry and Technology, 43 (3), 2023. doi: 10.1080/02773813.2023.2190594

Last update: 2024-11-19 21:32:44

No citation recorded.