DOI: https://doi.org/10.14710/jksa.23.12.432-439

Chitosan-CuO Nanoparticles as Antibacterial Shigella dysenteriae: Synthesis, Characterization, and In Vitro Study

1Pharmaceutical Chemistry, Bhakti Pertiwi High School of Pharmacy Science, Indonesia

2Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Sriwijaya University, Indonesia

3Department of Chemistry, Faculty of Mathematics and Natural Sciences, Sriwijaya University, Indonesia

Received: 5 Aug 2020; Revised: 11 Nov 2020; Accepted: 9 Jan 2021; Published: 31 Jan 2021.
Open Access Copyright 2020 Jurnal Kimia Sains dan Aplikasi under http://creativecommons.org/licenses/by-sa/4.0.

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Abstract

The synthesis of chitosan- CuO nanoparticles was studied. This research’s aims were biosynthesis CuO nanoparticles, synthesis of chitosan-CuO nanoparticles, and used as an antibacterial agent of Shigella dysenteriae. CuO nanoparticles and chitosan-CuO nanoparticles were characterized by FTIR spectroscopy and X-ray diffraction, respectively. CuO nanoparticle was synthesized by the reaction between leaf extract of sweet star fruit (Averrhoa carambola L.) and copper sulfate pentahydrate. Chitosan-CuO nanoparticles were synthesized by a heating method. The suspension of chitosan-CuO nanoparticles was used as an antibacterial agent with a paper disk method. The result showed that the Cu-O group at CuO nanoparticles was detected at a wavenumber of 503, 619, 767, and 821 cm-1. The crystallite size of the CuO nanoparticles was 4.25 nm. Cu-O group bonded at N-H and O-H groups and detected at 3406 cm-1 from the FTIR spectra of chitosan-CuO nanoparticles. The average inhibition zone of chitosan-CuO nanoparticles at concentration 2.500, 5.000, 7.500, and 10.000 ppm to Shigella dysenteriae were 13.57 ± 1.55; 14.90 ± 1.20; 15.97 ± 0.76 and 17.03 ± 1.80 mm, respectively.

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Keywords: Chitosan-CuO nanoparticles; characterization; Shigella dysenteriae
Funding: Sekolah Tinggi Ilmu Farmasi Bhakti Pertiwi

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Section: Research Articles
Language : EN
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