DOI: https://doi.org/10.14710/jksa.24.2.62-69

Immobilization of Crude Polyphenol Oxidase Extracts from Apples on Polypyrrole as a Membrane for Phenol Removal

1Department of Chemistry, Faculty of Sciences and Informatics, Universitas Jenderal Achmad Yani, Indonesia

2Department of Chemistry, Faculty of Mathematics and Natural Sciences, Institut Teknologi Bandung, Indonesia

Received: 1 Nov 2020; Revised: 8 Mar 2021; Accepted: 8 Mar 2021; Published: 15 Mar 2021.
Open Access Copyright 2021 Jurnal Kimia Sains dan Aplikasi under http://creativecommons.org/licenses/by-sa/4.0.

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Abstract

This research aims to make a polypyrrole (PPy) membrane and crude extract of polyphenol oxidase (PPO) as a membrane of mPPy/PPO apple extracts. The membrane of PPy/PPO-apple extract has been synthesized by the electrodeposition method. The electrolyte composition consists of a mixture of 0.10-0.20 M pyrrole (Py) and 50-100% PPO apple extract, which is stable using 50 mM of phosphate buffer solution at pH 6.80-7.00 and room temperature. The electrodeposition process is used 400 mesh steel gauze anode ST-304 and carbon plate cathode. Electrodeposition is carried out at potential = 5.00-6.00 V; current = 0.02-0.25 A; the distance from both electrodes = 1.00-2.00 cm for 300-500 seconds. The results from the deposition of PPy/PPO apple extract of the anode are a membrane of mPPy/PPO-apple extract, with total enzyme activity (U) = (957,1441, 2287 and 1754) using 2.00-5.00 mM phenol as a substrate which is measured based on the UV-visible spectrophotometric method. PPy and mPPy/PPO-apple extracts were characterized by SEM and SEM-EDS. The membrane of mPPy/PPO-apple extract can be used to remove phenol in industrial wastewater samples is 50-65% with a filtration capacity of 500 mL for 2 hours.

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Keywords: mPPy/PPO-apple extract; phenol; PPO activity; membrane
Funding: Directorate General of Higher Education of the Republic of Indonesia/RISTEK-BRIN; Research Grant of Pemprinas MP3EI-2015; LPPM Universitas Jenderal Achmad Yani

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Section: Research Articles
Language : EN
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  1. B. Marrot, A. Barrios-Martinez, P. Moulin, N. Roche, Biodegradation of high phenol concentration by activated sludge in an immersed membrane bioreactor, Biochemical Engineering Journal, 30, 2, (2006), 174-183 https://doi.org/10.1016/j.bej.2006.03.006
  2. A. Barrios-Martinez, E. Barbot, B. Marrot, P. Moulin, N. Roche, Degradation of synthetic phenol-containing wastewaters by MBR, Journal of Membrane Science, 281, 1, (2006), 288-296 https://doi.org/10.1016/j.memsci.2006.03.048
  3. Afilah Abd Gami, Phenol and its toxicity, Journal of Environmental Microbiology and Toxicology, 2, 1, (2014)
  4. Kai-Chee Loh, Tai-Shung Chung, Wei-Fern Ang, Immobilized-Cell Membrane Bioreactor for High-Strength Phenol Wastewater, Journal of Environmental Engineering, 126, 1, (2000), 75-79 https://doi.org/10.1061/(ASCE)0733-9372(2000)126:1(75)
  5. M. Victoria Martinez, John R. Whitaker, The biochemistry and control of enzymatic browning, Trends in Food Science & Technology, 6, 6, (1995), 195-200 https://doi.org/10.1016/S0924-2244(00)89054-8
  6. Christiane Queiroz, Antonio Jorge Ribeiro da Silva, Maria Lúcia Mendes Lopes, Eliane Fialho, Vera Lúcia Valente-Mesquita, Polyphenol oxidase activity, phenolic acid composition and browning in cashew apple (Anacardium occidentale, L.) after processing, Food Chemistry, 125, 1, (2011), 128-132 https://doi.org/10.1016/j.foodchem.2010.08.048
  7. Daniel Ferreira Holderbaum, Tomoyuki Kon, Tsuyoshi Kudo, Miguel Pedro Guerra, Enzymatic browning, polyphenol oxidase activity, and polyphenols in four apple cultivars: dynamics during fruit development, HortScience, 45, 8, (2010), 1150-1154 https://doi.org/10.21273/HORTSCI.45.8.1150
  8. Francesca Taranto, Antonella Pasqualone, Giacomo Mangini, Pasquale Tripodi, Monica Marilena Miazzi, Stefano Pavan, Cinzia Montemurro, Polyphenol oxidases in crops: biochemical, physiological and genetic aspects, International journal of molecular sciences, 18, 2, (2017), 377 https://doi.org/10.3390/ijms18020377
  9. Zhiguo Li, Colin Thomas, Quantitative evaluation of mechanical damage to fresh fruits, Trends in Food Science & Technology, 35, 2, (2014), 138-150 https://doi.org/10.1016/j.tifs.2013.12.001
  10. Martina Persic, Maja Mikulic-Petkovsek, Ana Slatnar, Robert Veberic, Chemical composition of apple fruit, juice and pomace and the correlation between phenolic content, enzymatic activity and browning, LWT - Food Science and Technology, 82, (2017), 23-31 https://doi.org/10.1016/j.lwt.2017.04.017
  11. Kyoung Mi Moon, Eun-Bin Kwon, Bonggi Lee, Choon Young Kim, Recent trends in controlling the enzymatic browning of fruit and vegetable products, Molecules, 25, 12, (2020), 2754 https://doi.org/10.3390/molecules25122754
  12. Anceu Murniati, Buchari Buchari, Suryo Gandasasmita, Zeily Nurachman, Synthesis and characterization of polypyrrole polyphenol oxidase (PPy/PPO) on platinum electrode, Research Journal of Pharmaceutical, Biological and Chemical Sciences, 3, (2012), 855-864
  13. Manik A. Chougule, Shailesh G. Pawar, Prasad R. Godse, Ramesh N. Mulik, Shashwati Sen, Vikas B. Patil, Synthesis and Characterization of Polypyrrole (PPy) Thin Films, Soft Nanoscience Letters, 1, 1, (2011), 1-5 https://doi.org/10.4236/snl.2011.11002
  14. Abdirahman Yussuf, Mohammad Al-Saleh, Salah Al-Enezi, Gils Abraham, Synthesis and characterization of conductive polypyrrole: the influence of the oxidants and monomer on the electrical, thermal, and morphological properties, International Journal of Polymer Science, 2018, (2018), Article ID 4191747 https://doi.org/10.1155/2018/4191747
  15. A. Elif Böyükbayram, Senem Kıralp, Levent Toppare, Yusuf Yağcı, Preparation of biosensors by immobilization of polyphenol oxidase in conducting copolymers and their use in determination of phenolic compounds in red wine, Bioelectrochemistry, 69, 2, (2006), 164-171 https://doi.org/10.1016/j.bioelechem.2006.01.006
  16. André Brisolari, Débora Gonçalves, Immobilization of tyrosinase from avocado crude extract in polypyrrole films for inhibitive detection of benzoic acid, Chemosensors, 2, 3, (2014), 182-192 https://doi.org/10.3390/chemosensors2030182
  17. Huseyin Bekir Yildiz, Senem Kiralp, Levent Toppare, Yusuf Yagci, Immobilization of tyrosinase in poly(ethyleneoxide) electrodes and determination of phenolics in red wines, Reactive and Functional Polymers, 63, 2, (2005), 155-161 https://doi.org/10.1016/j.reactfunctpolym.2005.02.016
  18. Qaisar Ameer, Samuel B. Adeloju, Development of a potentiometric catechol biosensor by entrapment of tyrosinase within polypyrrole film, Sensors and Actuators B: Chemical, 140, 1, (2009), 5-11 https://doi.org/10.1016/j.snb.2009.03.056
  19. Meysam Sharifirad, Abdollah Omrani, Abbas Ali Rostami, Maryam Khoshroo, Electrodeposition and characterization of polypyrrole films on copper, Journal of Electroanalytical Chemistry, 645, 2, (2010), 149-158 https://doi.org/10.1016/j.jelechem.2010.05.005
  20. Anceu Murniati, Buchari Buchari, Suryo Gandasasmita, Zeily Nurachman, Nury Nurhanifah, Characterization of Polyphenol Oxidase Application as Phenol Removal in Extracts of Rejected White Oyster Mushrooms (Pleurotus ostreatus), Oriental Journal of Chemistry, 34, 3, (2018), 1457-1468 http://dx.doi.org/10.13005/ojc/340336
  21. Marcel Mulder, Basic Principles of Membrane Technology, 2nd ed., Springer Netherlands, 2012, https://doi.org/10.1007/978-94-009-1766-8
  22. Joseph Wang, Analytical Electrochemistry, Wiley-VCH, Germany, 1994
  23. Anceu Murniati, Buchari Buchari, Suryo Gandasasmita, Zeily Nurachman, Sintesis dan Karakterisasi Polipirol pada Elektroda Kerja Kasa Baja dengan Metode Voltametri Siklik, Jurnal Sains Materi Indonesia, 13, 3, (2018), 210-215
  24. Saïd Sadki, Philippe Schottland, Nancy Brodie, Guillaume Sabouraud, The mechanisms of pyrrole electropolymerization, Chemical Society Reviews, 29, 5, (2000), 283-293 https://doi.org/10.1039/A807124A
  25. Serge Cosnier, Biomolecule immobilization on electrode surfaces by entrapment or attachment to electrochemically polymerized films. A review, Biosensors and Bioelectronics, 14, 5, (1999), 443-456 https://doi.org/10.1016/S0956-5663(99)00024-X
  26. Fang Liu, Jin-Hong Zhao, Xin Wen, Yuan-Ying Ni, Purification and structural analysis of membrane-bound polyphenol oxidase from Fuji apple, Food Chemistry, 183, (2015), 72-77 https://doi.org/10.1016/j.foodchem.2015.03.027
  27. Stephanie G. Burton, Aileen Boshoff, Wade Edwards, Peter D. Rose, Biotransformation of phenols using immobilised polyphenol oxidase, Journal of Molecular Catalysis B: Enzymatic, 5, 1, (1998), 411-416 https://doi.org/10.1016/S1381-1177(98)00020-4
  28. W. Edwards, R. Bownes, W. D. Leukes, E. P. Jacobs, R. Sanderson, P. D. Rose, S. G. Burton, A capillary membrane bioreactor using immobilized polyphenol oxidase for the removal of phenols from industrial effluents, Enzyme and Microbial Technology, 24, 3, (1999), 209-217 https://doi.org/10.1016/S0141-0229(98)00110-0
  29. W. Edwards, W. D. Leukes, P. D. Rose, S. G. Burton, Immobilization of polyphenol oxidase on chitosan-coated polysulphone capillary membranes for improved phenolic effluent bioremediation, Enzyme and Microbial Technology, 25, 8, (1999), 769-773 https://doi.org/10.1016/S0141-0229(99)00116-7
  30. Jian Shao, Huimin Ge, Yumin Yang, Immobilization of polyphenol oxidase on chitosan–SiO2 gel for removal of aqueous phenol, Biotechnology Letters, 29, 6, (2007), 901-905 https://doi.org/10.1007/s10529-007-9329-2
  31. Fabian Weber, Maike Passon, Chapter 7 - Characterization and Quantification of Polyphenols in Fruits, in: R.R. Watson (Ed.) Polyphenols in Plants (Second Edition), Academic Press, 2019, https://doi.org/10.1016/B978-0-12-813768-0.00007-4

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